To define the epidemiology of Bovine Ephemeral Fever (BEF) in Okinawa prefecture, the glycoprotein geneof ten Okinawan BEF virus isolates between 1988 and 2004 were sequenced and subjected to phylogeneticalanalysis with other available BEF viruses. Phylogenetical analysis revealed that the Okinawan and Taiwaneseisolates formed a monophyletic group that was separated from the vaccine (YHL) strain and the Australian isolatesand were further divided into two clusters, those isolated in the 1980s and those isolated in the 1990 and2000s. High nucleotide identities were observed between the Okinawan and Taiwanese isolates in the sameyear, indicating an epidemiological link between the BEF outbreaks in Okinawa prefecture and those in Taiwan. Although four amino acid substitutions in the neutralizing epitopes of the glycoprotein were foundbetween the YHL strain and Okinawan isolates, immune serum from vaccinated cattle equally neutralized boththe Okinawan isolates and the YHL stra in in the neutralization test.
Experiments were conducted to investigate whether pigs could be infected with bovine viral diarrhea virus (BVDV) by transfer into a BVDV-contaminated environment. Pigs in test groups 1 and 2 were transferred intoa pigsty in which culture medium supernatant from cells used to propagate wild-type BVDV and urine from persistentlyinfected calves were scattered over the floor and chow. Test group 2 also received an oral BVDV gavage.A positive control group received a BVDV solution through intramuscular inoculation. A negative controlgroup was kept in a pigsty in which a culture medium that did not contain BVDV was dispersed. For pigsin test groups 1 and 2 and the positive control group, BVDV was isolated from sera on days 3 to 14 and an elevated BVDV neutralizing antibody was measured starting on day 14. The data shows that a BVDV-contaminatedenvironment can be a source of BVDV infection for pigs.
An ovarian granulosa cell tumor was suspected based on the multilobulated appearance of an enlarged ovaryin ultrasonography, and was surgically removed in two Holsteins.A granulose cell tumor was found in theremoved ovary through a histopathological examination.The first AI was conducted when estrous signsappeared on 11 or 37 days after removal of the affected ovary and both cows conceived with the second AI, conducted 21 daysafter the first.
nine-year-old castrated male Japanese domestic cat presented ap Petite loss and vomiting, as well as palemucose mem'brane and pain in the right renal region. Plain X-ray revealed masses in the caudal part of the thoraciccavity and enlargement of the right kidney.Excretory urography failed to produce contrast images of theright kidney. Right nephrectomy and an incisional biopsy of the intrathoracic masses were conducted on day 10.The cranial side of the right kidney was enlarged, and the intrathoracic masses were blackish and slightlyhard. The right kidney and the intrathoracic masses presented similar histopatholgical features and werediagnosed as hemangiosarcoma (HSA).The cat's condition suddenly deteriorated on day 15 and it died.Necropsy was not conducted according the request of the owner, which hindered identification of the causeand prevented a detailed analysis of the tumor pathology. The cat showed pain in the renal region by palpation, and hematology revealed nonregenerative anemia.A renal HSA should be considered as a diagnosis fora cat presenting with similar clinical signs.
Hepatic rupture and hemoperitoneum were found in postmortem examinations of two cats seropositive forfeline leukemia virus. One cat had clinically shown severe enlargements of the liver and kidneys, severe hyperbilirubinemia, and cytological findings indicative of renal lymphoma, and had died without responding tochemotherapy. Another cat had been receiving treatments for chronic anemia due to myelodysplastic syndrome, and had died showing hyperbilirubinemia. In both cases, histopathological examinations revealedsevere amyloid deposits in the liver. Milder amyloid deposits were also found in the kidney and other organs, and a diagnosis of systemic amyloidosis was made. Hepatic ruptures in these two cats were thought to be associatedwith amyloidosis.
five-year-old castrated male ferret weighing 1.25 kg was presented to the Animal Medical Center of the Nippon Veterinary and Life Science University with a one-month history of exercise intolerance and one week ofintermittent coughing. A physical examination revealed that tachycardia and arrhythmia were ausclutated.Electrocardiography revealed ventricular parasystole. Although mild cardiac enlargement was observed bythoracic radiography, a reduced diastolic internal diameter of the left ventricle and fractional shortening wereconfirmed by echocardiography. Oral temocapril and atenolol were initiated, however the ferret died suddenlyafter 192 hospital days.
A seven-year-old neutered male cavalier King Charles spaniel presented with exercise intolerance, cough anddyspnea.A grade 5/6 cardiac systolic murmur was audible in the area of the left cardiac apex.On a thoracicradiograph, the cardiac silhouette was remarkably enlarged and pulmonary edema was observed.Echocardiographyshowed marked mitral regurgitation and an enlarged left atria (LA/Ao: 3.5).Based on these findings, the dog was diagnosed with severe mitral regurgitation, following a rupture of the chordae tendineae.The mitral regurgitation was treated with mitral valve plasty using cardiopulmonary bypass.Two months aftersurgery, the dog was clinically normal and an echocardiography revealed that mitral regurgitation was conspicuouslyreduced.This case suggested that mitral regurgitation in cavalier King Charles spaniels can betreated with mitral valve plasty.
The green livers (GL) of broiler chickens were examined for a connection with osteomyelitis. More than 95%of broiler chickens with GL had osteomyelitis, often with multiple occurrences or occurrences in the proximalpart of the tibia and head of the femur. Staphylococcus hyicus, Salmonella Infantis, Escheria coli were often isolatedfrom osteomylitis lesions. These bacteria were also isolated from visceral organs. This suggests thatbroiler chickens with GL are septic and GL can be an effective marker for septic broiler chickens.