A seroepidemiological survey of
Ehrlichia canis and
Anaplasma phagocytophilum infection in dogs in Japan was performed by using a combination of a classical immunofluorescence antibody assay (IFA) and a new enzyme immune assay (EIA) kit that uses a specific recombinant major surface protein of the pathogens. Although 64 (5.3%) of 1,206 dogs showed IFA antibodies against
E. canis with titers of 20 or more, all 64 were negative for
E. canis via the EIA kit. The results of IFA were believed to be false positive, resulting from infection with closely related
Ehrlichia species. Three (0.2%) of 1,206 dogs showed IFA antibodies against
A. phagocytophilum with titers of 20 or more. One of these three dogs also showed positive for
A. phagocytophilum in the EIA kit. It was suggested that the dog might be infected with
A. phagocytophilum in Japan.
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