Weekly changes in serum progesterone (P
4) concentrations from 26 normal pregnant mares were determined through time-resolved fluorescence immunoassay (TR-FIA). A comparison was also made with changes in seven mares with early pregnancy loss at approximately 5 and 9 weeks of gestation. Mean concentrations of P
4 showed biphasic changes with peaks at two weeks (12.3 ng/m
l) and at 7-15 weeks (14.8-17.0 ng/m
l) of gestation, which were associated with the formation of primary and supplementary corpora lutea. Thereafter, P
4 levels remained low (3-4 ng/m
l) during mid to late gestation, before showing gradual increases 5 weeks before parturition, followed by an abrupt fall after parturition. There is no significant difference in the serum con centrations of P
4 between mares with normal pregnancies and four mares with pregnancy loss at around five weeks from 0 to 4 weeks of gestation, whereas serum concentrations of P
4 were significantly lower in three mares with pregnancy loss at around nine weeks than those in mares with normal pregnancies two weeks prior to fetus loss. These pregnancy losses resulted in the maintenance of circulating P
4 levels at a range of 3 to 6 ng/m
l, due to a lack of luteolysis. The present result demonstrated weekly changes in P
4 levels during preg nancies in mares. TR-FIA is a practical and rapid method to determine equine P
4 levels. These results also suggest that P
4 may become a good indicator as a prediction of early fetus loss in mares.
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