In this study, we examined pig feces for the presence of the eggs of Ascaris suum, Trichuris suis, and Oesophagostomum sp. Between September 2005 and March 2007, we analyzed 4,221 samples of pig feces that were collected from 150 pig farms across Japan. The sucrose floatation method was used to detect the eggs. The rate of egg detection was 2.0% for A. suum, 2.1% for T. suis, and 2.5% for Oesophagostomum sp. For A. suum,the egg detection rate was significantly higher in sows and boars than in fatteners. For T. suis, the egg detection rate was higher in fatteners and boars than in sows. In the case of Oesophagostomum sp., the rate was higher in sows than in fatteners (P ＜0.05). The eggs of more than one nematode species were detected in 39.3% of the farms, and the eggs of two or three nematode species were detected in 15.3% of the farms. These results clearly suggest that the parasites still remain on pig farms across Japan and that it is important to take suitable steps to prevent the occurrence of parasites on these farms.
A four-day-old Japanese Black Cattle calf presented with severe watery diarrhea, which lead to depression and bloody stool, followed by death, in Ishigaki Island, Japan on June 2007. Vibrio cholerae was isolated from the brain, heart, lungs and major organs in a pure culture. The strains were identified as serogroup O135. Immunohistochemical examination revealed a large number of V. choleraeo O135 antigens in the brain, lungs and thymus. The isolates do not produce cholera enterotoxin, while harbor hlyA and toxR genes. An epidemiological investigation showed that V. cholerae O135 was not isolated in environmental samples collected from the farm, whereas V. cholerae O14, O19, O27 and O170 were isolated from the seawater samples around coastal areas in remote islands respectively.
A Holstein cow (5 years and 3 months old) presented with hind limb paresis and was unable to stand. Bovine leukosis was suspected given the enlargement of cervical lymph nodes and existence of an abdominal mass detected by rectal palpation; however, it could not be confirmed by blood examination and fine needle aspiration of cervical lymph nodes. Pathological examination revealed a multinodular mass (60 × 40 × 20 cm) on the dorsal abdomen that was invasive into the iliopsoas muscle. A portion of the mass invaded the vertebral canal between the 5th and 6th intervertebral foramina and compressed the spinal cord and nerves. Histopathological examination revealed the mass to be T-cell lymphoma based on CD3 positivity. Given that antibodies against bovine leukemia virus were not detected, the case was diagnosed as sporadic type bovine leukosis.
A 6-year-old neutered hybrid male rabbit (Oryctolagus cuniculus, weight : 1.28kg) was brought into Onuma Animal Hospital with chief complaints of polyposia and polyuria. The volume of water intake was 760 (ml/head/day), six times greater than normal. The amount of urine production per day was 1.5 times larger than the normal value of 530 (ml/head/day). The urine specific gravity was low, at 1.001. After excluding several different diagnoses, the animal was diagnosed with partial diabetes insipidus based on the results of a modified water deprivation test. An intranasal synthetic vasopressin derivative was administered (1 drip, 24 hours),and the urine specific gravity, volume of water intake, and urinary production per day became 1.020 (median),346 ml/head/day (median), and 200 ml/head/day (median), respectively. A diagnosis of partial central diabetes insipidus was consequently made.
A four-month-old male Airedale terrier presented for an evaluation of a cataract in the right eye (OD). A complete ophthalmic examination and ultrasonography (US), including color Doppler, were performed. Leukocoria was observed in a general ophthalmic examination of the OD. The fundus of the OD showed partial signs of leukocoria. A B-mode US of the OD demonstrated a small hyperechoic posterior subcapsular lens, funnel shaped echodense structure and hyperechoic strand from the retrolental structure to the optic disc area. Based on these findings, a diagnosis of Stades's grade 3 persistent hyperplastic tunica vasculosa lentis/persistent hyperplastic primary vitreous (PHTVL/PHPV) with posterior subcapsular cataract was made. Moreover,through color Doppler imaging, it was suspected that blood flow was not presented in the vestigial structures. The US was useful in assessing image analysis and making a pathological classification, and it also assisted in the prognostic determination of PHTVL/PHPV.
An eight-year-old spayed female Labrador retriever with heamaturia and an enlarged left kidney underwent nephrectomy to treat a suspected renal tumor. The surgically resected left kidney was diagnosed with extraskeletal osteosarcoma. The dog was treated with carboplatin postoperatively and has been in good condition throughout the 248-day postoperative period, with neither local recurrence nor distant metastasis.