More than ten cattle were diagnosed with bovine brucellosis in each of the last several years using serological testing during import quarantine. However,
Brucella abortus has not been isolated from the samples of the cattle. In 2008, a bacterium biochemically closed to the genus
Brucella was isolated from the lung of one of the feeder cattle imported from Australia, which was serologically diagnosed with brucellosis during import quarantine. The bacterium tested positive in a plate agglutination test for Brucella-specific antisera, and tested positive in a PCR assay for
Brucella spp. The bacterium was identified as
Ochrobactrum intermedium by PCR assay of the
recA genes and genetic analysis using 16s rRNA. The PCR assay of
recA genes and the growth on SSagar is useful for identifying
Ochrobactrum spp. and
Brucella spp.
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