A male 7.5-month-old Japanese Black calf, wobbling on his hind limbs and showing a symmetric hopping gait (bunny hop), was investigated clinically and histopathologically. No abnormality was revealed in radiography and myelography of the spine and spinal cord. At autopsy, the L4 segment of the spinal cord had a centrally located cavity. Microscopically, the central canal between L4 and L6 was bifurcated. These segments had a pair of spinal ganglions, and the calf was diagnosed with incomplete diplomyelia.
A serodiagnostic test specific to strangles was used in an outbreak of strangles at a horse farm in Fukushima Prefecture, and genetic analysis of the Streptococcus equi subsp. equi isolates was performed. In the first examination, conducted on all horses on the farm, 18.7% of nasal samples collected from 91 horses were PCR positive for S. equi, and S. equi was isolated from seven horses. Positive rates of PCR and bacterial isolation decreased gradually after the third examination. Finally, negative results for the isolation of S. equi strains were obtained from the fourth to sixth consecutive examinations conducted at monthly intervals. We therefore considered that strangles had been eradicated from the farm. Two kinds of seM genotype were detected in all S. equi isolated. Application of the serodiagnostic test differentiated affected, unaffected horses, and horses that had recovered from the disease at the time of examination. This report provides valuable information for the effective control of strangles on farms.
A Japanese Brown freemartin (21 months old) was clinically diagnosed with hydroneprosis and renal failure. A pathological examination revealed severe masculinization of the urogenital organs, pyonephritis and urolithiasis. These findings suggested that it was a rare case of a freemartin with a post-renal failure and morphological anomaly of the urinary tract, complicated by a bacterial infection and urolithiasis.
In the present study, serum samples were collected from feral raccoons (n ＝186) captured in the southern part of Osaka Prefecture (A) and the eastern part of Hyogo Prefecture (B), as well as from pet dogs (n＝52) in the same regions. Anti-Leptospira interrogans antibodies were evaluated with a microscopic agglutination test (MAT), using five major L. interrogans serovars as antigens. In both regions, the most frequently detected serovar was hebdomadis (A : 31.5% and B : 51.5%) in raccoons, but these serovars were not detected from the pet dogs. L. interrogans-specific PCR analysis revealed that eight out of 66 (12.1%) raccoons were infected with the pathogen. These observations revealed that a high percentage of feral raccoons are infected with Leptospira interrogans, but that transmission may not occur between the raccoons and dogs.
We administered lomustine (CCNU) to a 12-year-old Shih Tzu with T-cell type cutaneous lymphoma with swelling of the lower lip and mandibular lymph node. The treatment was ineffective and there was no reduction in the swelling in either area. Next, we carried out five radiation treatments (4Gy once every two days) with a total dose of radioactivity of 20Gy. Following the wishes of the owner, we did not perform further treatment. However, after several radiation treatments, there was a noticeable reduction in the swelling of the lower lip and the mandibular. The tumors of the lower lip and mandibular lymph node eventually increased and the dog died 234 days after the initial treatment.
A seven-month-old cat with severe neurological symptoms was diagnosed with non-effusive feline infectious peritonitis (FIP) following a pathological examination. Grossly, the brain showed marked congestion and edema. Multiple creamy white nodules were located in the renal cortex. Histologically, pyogranulomatous lesions were found in the renal cortex and the central nervous system (CNS) parenchyma around the ventricles and choroid plexus, whereas a fibrin thrombus in the sinus of dura mater and diffuse congestion were observed in the cerebrum. FIP virus-positive immunolabelings were detected in the cytoplasm of the macrophages in the lesions. These findings suggest that inflammation located in the periventricular areas and choroid plexus due to FIP virus infection induced fibrin thrombus formation, leading to a circulatory disturbance in the sinus of the dura mater.