A total of 39 Mannheimia haemolytica from cattle in the Tokachi area from 1994 to 2006 were examined for serotyping, with an antibiotics sensitivity test and pulse field gel electrophoresis (PFGE). Twenty-one isolates (53.8%) were serotype 1, sixteen isolates (41%) were serotype 6 and two isolates were serotype 2 and untypable. Serotype 1 strains had been isolated continually, but had serotype 6 strains for the first time in 1999. Serotype 6 strains were isolated in greater number than serotype 1 strains starting 2004. Eighteen isolates of serotype 1 and 15 isolates of serotype 6 showed the same PFGE pattern. It was suggested that closely related isolates of serotype 1 and 6 were prevalent in the Tokachi area. Fifteen isolates of serotype 6 were multidrug resistant to antibiotics including SM, KM, CP, NA, ERFX. MIC90s of NA, ERFX and CP were also high in serotype 6 strains. Most of these multidrughresistance serotype 6 strains were isolated from Holstein or F1 (Holstein × Japanese Black Beef) calves for meat.
In summer 2006, splenomegaly and splenic mottling of brand meat chickens were observed, when 0.53-1.88% of birds were condemned for a typical Marek's disease. In summer 2007, a similar condition was observed and 50 spleens obtained at slaughter from one of the flocks were examined. Histologically, reticuloendothelial cells hyperplasia with intranuclear inclusion boies were observed and ultrastructurally, adenovirus particles were detected. A PCR assay showed that the spleens were positive for the hexon gene of group Ⅱavian adenovirus (GⅡAAV). The sequence of the PCR product was identical to the avirulent turkey hemorrhagic enteritis virus strain Virginia with 97.3% homology. The extract of the above spleens was inoculated intravenously in 8-week-old chickens. Four days post inoculation, splenomegaly was observed and the spleen,thymus, and bursa of Fabricius were positive for the GⅡAAV gene.
The goal of the present study was to investigate the impact of rearing school-owned animals on the psychological development of children. Three questionnaires of 768 fourth-grade elementary school pupils were conducted (T1: before starting the rearing, T2: at the end of one year's rearing, T3: one year after the end of the rearing). It was found that the group that reared school-owned animals appropriately, compared to the control group that did not engage in animal rearing, showed a smaller decrease in school adjustment (SA) during T1 through T2, and a smaller decrease in SA, sympathy for animals (SFA), kindness to people (KTP), and prosocial attitude (PA) during T1 through T3. The children who engaged in appropriate animal rearing in school only, compared to those children who reared animals in their homes only, showed a smaller decrease in PA during T1 through T3. The group that reared school-owned animals inappropriately showed a greater decrease in SFA, KTP, and PA during T1 through T2, and a greater decrease in PA during T1 through T3 compared to the control group. It was suggested that animal-assisted education through the appropriate rearing of school-owned animals has an impact on the development of school adjustment, consideration for animals and people.
A 7-year-old male Miniature Dachshund was referred to the Veterinary Medical Center of Osaka Prefecture University because of sudden severe uremia that had not responded to conventional therapy. The cause was not specified at the first medical examination, but hemodialysis was undertaken to control the clinical symptoms related to severe uremia. The dog was stabilized with repeated hemodialysis. Further intensive examination was declined and hemodialysis was continued to maintain QOL in accordance with the client's wishes. Clinical signs related to severe uremia improved after treatment. The dog eventually died due to a suspected acute infection, and end stage renal failure due to bilateral renal dysplasia was diagnosed based on the necropsy result. In this case, hemodialysis was highly effective for controlling uremia and improving the associated symptoms. As a result, hemodialysis may be considered as an option for improving the QOL of renal dysplasia cases.