The prevalence of
Sarcocystis cysts in the myocardium of cattle slaughtered in Gifu Prefecture was evaluated. The specimens were identified as
Sarcocystis cruzi by PCR-direct sequencing and a morphological examination of the cyst. The cyst wall was smooth and thin. The cysts measured 273.9~936.8μm (n = 33, with an average of 482.9μm)× 78.9 ~242.1μm (143.4μm) in size.
Sarcocystis cysts were detected in 94.3% (50/53head) of old dairy cows (Holstein), 53.6% (30/56) of Japanese black beef cattle, and 32.3% (20/62) of crossbred beef cattle. The average numbers of cysts detected per tissue section were 11.1 (range 1h141) for Holsteins,4.1 (1h23) for Japanese black cattle, and 6.0 (1h41) for crossbred cattle, suggesting that some individual cattle in all breeds possess high numbers of cysts. Immunostaining with antiserum against the toxicity protein of
S. fayeri, a cause of food poisoning due to raw horsemeat, showed a positive reaction around
S. cruzi bradyzoites in the cysts.
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