A female wild boar that had died of a debilitation was pathologically examined. In addition to an internal and external parasitic lesion, basophilic intranuclear inclusion bodies were observed systemically in reticuloendothelial cells. Immunohistochemical, transmission electron microscopic, virological and genetical investigations were subsequently conducted, and the case was diagnosed as porcine cytomegalovirus (PCMV) infection. PCMV infection among wild boars in Yamaguchi prefecture was further investigated, and the PCMV gene was identified in 2 of 30 serum samples collected in 2011. These three PCMV sequences (one from the deceased case and two from serum samples) were genetically distinct to each other, suggesting genetic diversity among PCMV strains in wild boars. This is the first detailed pathological report on PCMV infection in wild boars.
A normally born Japanese black calf showed astasia and subsequently died 4 days after birth. Mannheimia spp. were isolated from the brain, lungs, kidneys, and spleen by bacteriological examination. Although the isolate was initially classified as M. haemolytica, it was later classified as M. varigena (M.v) via 16S ribosomal RNA gene sequencing. Histopathological examination revealed suppurative meningitis, suppurative uveitis, and acute necrotizing pneumonia. M.v-immunopositive antigens were detected in the brain, eyeballs, and lungs via immunohistochemical analysis. M.v was isolated also from the dam of the dead calf and was considered the source of infection. Further investigations are needed to establish the virulence and carriers of M.v.
A 4-year-old spayed ferret was presented with a chief complaint of anorexia for 3 days. Physical examination and abdominal radiography revealed a very large mass in the left kidney region. A laparotomy revealed that the mass occupied the entire abdominal cavity and was severely adhered to the adjoining organs. Because the left kidney was buried in the mass, a combined resection with the kidney was performed. The left adrenal gland was not found during the surgery. Microscopic examination of the mass revealed a well-differentiated neoplasm derived from all three germ cell layers. The neoplasm contained various mature tissues including the skin and skin appendages, bone, cartilage, nervous system, intestinal epithelium, and optic cup-like structures. From these findings, the neoplasm was diagnosed as a teratoma. No adrenal tissue was seen in the neoplatic tissue. The origin of the teratoma remained unclear. The left adrenal gland was suspected as the origin, because it was not found during the surgery.
A 4-month-old female Chihuahua was referred to our hospital for investigation of underdevelopment, ascites, and hyperammonemia. Ultrasonography with color Doppler imaging and nonselective arteriography showed a hepatic arteriovenous fistula with multiple acquired portosystemic shunts (MAPSS). A lobectomy of several hepatic lobes, including hepatic arteriovenous fistula, and attenuation of some MAPSS were performed after administering medical treatment for 7 days. After the operation, the general condition improved, but hyperammonemia and mild hepatic failure findings persisted. Hence, the medical treatment for hyperammonemia was continued. Subsequent reoperations for to further attenuateion of MAPSS were performed 48 days after the surgery. Considerable improvement in hyperammonemia and hepatic failure was noted after the second surgery. A liver biopsy performed 18 months after the primary operation showed the disappearance of the histopathological abnormality.
We examined changes in the count of Pseudomonas spp., which are bacteria that play a significant role in meat spoilage, during processing at a slaughterhouse. Pseudomonas spp. counts on porcine carcasses and in water flowing over the bodies and carcasses were measured. On the upper part of the carcass, Pseudomonas spp. counts ranged from 2.0×100 to 101 cfu/cm2 before full skinning through to the pretreatment process, but decreased to undetectable levels before carcass washing. On the lower part of the carcass, Pseudomonas spp. counts were not detected immediately after partial skinning, but they increased after full skinning, and ranged from 2.0×100 to 101 cfu/cm2 before carcass washing. In the water collected from the carcass we detected Pseudomonas spp. at 103 to 104 cfu/ml. Contamination on the carcass was thus spread by the water.