We performed two experiments to determine the effect of intrauterine administration of chitosan acetic acid solution (chitosan solution) on the conception rate after synchronization of estrus-ovulation. In the first experiment, the cows were given intrauterine administration of chitosan solution at a dose of 200 mg/50 m
l. Seven days after the chitosan solution administration, the cows were given variant CIDR-synch (chitosan CIDR group : n=106). The conception rate of cows with CIDR-synch only (control group : n=101) was 44.5%, whereas the rate for chitosan-treated cows was 59.4% (
P<0.05). In the second experiment, the subjects were postpartum Holstein cows, which showed no clear estrus until after parturition. The cows were given intrauterine administration of a chitosan solution. Rectal examinations were performed 7-14 days after administration, and cows with corpus luteum were given intramuscular injections of PGF
2α (PG) to induce estrus, and were artificially inseminated (chitosan group : n=27). The conception rate of cows with PG only (n=29) was 37.9%, whereas the rate for chitosan-treated cows was 74.1%. The rate for chitosan-treated cows was thus significantly higher than that for cows with PG only (
P<0.01). These findings suggest that with intrauterine administration of the chitosan solution before synchronization of estrus-ovulation, conception rates improved.
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