The poultry red mite, Dermanyssus gallinae, was collected from 139 poultry farms in 32 prefectures in Japan during the period from 2007 to 2010, and from 119 poultry farms in 29 prefectures in Japan from 2011 to 2014. The development of resistance in these D. gallinae mites against commercial acaricides that had been used for the control of D. gallinae (three carbamates, one pyrethroid and three organophosphates) and commercial insecticides used for the control of flies (two pyrethroids, two combinations of pyrethroid and organophosphate) was examined. As a result, D. gallinae mites showed a resistance against all acaricides and insecticides examined in this study, but the development of resistance against carbamate acaricide (carbaryl), organophosphate acaricide (trichlorfon), and combination insecticide (fenitrothion, permethrin, and phthalthrin) appeared to be weaker than those of other acaricides and insecticides. The development of resistance against all commercial acaricides that target D. gallinae mites control was confirmed in 19 out of 139 (13.7%) poultry farms from 2007 to 2010, and 22 out of 119 (18.5%) poultry farms from 2011 to 2013.
A six-year-old female miniature pig was presented after a week-long episode of anorexia. A tumor was identified in the abdominal cavity by an X-ray examination.Surgical removal of the tumor was conducted on day seven after the initial examination.Because of the position and the histopathological findings of the tumor, the diagnosis was for a malignant granulosa cell tumor. After the operation, the case demonstrated no symptoms for approximately 200 days. On day 223 after the initial examination, the case study suddenly died after displaying neurological symptoms. At necropsy, a nodule was found in the pituitary gland. However, no recurrence of the tumor in the abdominal cavity was observed. Because the histopathological findings of the nodule were the same as those of the abdominal tumor and the tumor cells were κ-light chain-positive, plasmacytoma was the revised diagnosis of the tumor. Surgical removal of the tumor improved quality of life in the case study, and no symptoms were observed for approximately seven months. Therefore, we concluded that the surgical treatment resulted in a good prognosis for this case study.
At the Meat Inspection Station, Kanagawa Prefectural Government, 17 strains isolated from bovine and swine valvular endocarditis previously identified as Gemella, Granulicatella or pyridoxal-dependent Gram-positive coccus by commercial identification kits, were all identified as Helcococcus ovis (H. ovis) by a sequence analysis of the 16S rDNA. The data indicates that H. ovis may therefore cause not only bovine valvular endocarditis, but also swine valvular endocarditis. Furthermore, H. ovis was also detected by immunostaining in the warts from which the organism had been isolated. In addition, we developed H. ovis specific PCR, and all the 17 strains examined were specifically identified as H. ovis and discriminated from 27 other strains of gram-positive cocci. This PCR method is therefore considered to be a useful tool for the rapid identification of H. ovis.