Three calves (37 to 40 days old) died acutely in Shiga Prefecture in 2005. During postmortem examinations characteristic findings were observed in all cases of swelling of the mesenteric lymph nodes. Histopathological examination revealed suppurative lesions in mucous membranes of the small intestine and the large intestine. Mannheimia (M.) varigena (M. v)-immunopositive antigens were detected in the mucous membrane of the intestines. M. sp. was isolated through bacteriological examination, and they was classified as M. v and unclassified as five species of M. (M. sp.) via 16S rRNA gene sequencing. Escherichia coli, Clostridium perfringens, coronavirus, and coccidia were also isolated at that time. It was concluded that the enteritis was related to the M. v and M. sp. infections.
Primary soft tissue lymphoma (STL) was diagnosed using a punch biopsy in two cats with swelling and pain of the forelimb. When CT and MRI scans were used on one of two cats, the localization of the lesions could be clearly visualized. Chemotherapy was given to the two cats based on the detection of massive and invasive lesions during the examination of the imaging. However, the two cats finally died from hyperammonemia and poor conditions from adverse effects within a short period after treatment. It is difficult to distinguish STL from post-traumatic inflammation in cats because of the striking similarity of the clinical symptoms. Therefore, the study suggested that an open biopsy should be required for a definitive diagnosis and precise early treatment.
An eight-month-old spayed female Italian greyhound with a body weight of 4.6 kg was referred to our hospital for examination due to lameness in the right forelimb. An x-ray and computed tomography showed severe deformity of the right radius and ulna. The deformity of the right forelimb was determined to have been caused by a past radioulnar fracture. The degree of deformity was severe and complex. It was difficult to locate an accurate landmark for corrective osteotomy using conventional computed images, which were predicted to lead to malalignment. We constructed 3D printed models from computed tomography, performed a simulated operation, and made an implant through this method. After the simulated operation, a good prognosis for correction was confirmed and we actually performed the operation. After the surgery, the lameness was resolved and at the time of this writing, the dog is clinically normal with completely healed bones.
A microbiological survey was conducted to clarify the hygienic condition of fabric for companion animals, such as dogs and cats, kept in homes in Japan. Moreover, the effect of washing was found to be reduced for fabric contaminated microbial pathogens that were used by companion animals. The average amount of bacteria and fungi in the fabrics used for two weeks to one year were 5.7 and 4.1 log CFU/100 cm2 respectively. Of the 23 samples, 10 (43.4%) were contaminated with Escherichia coli. Pasteurella multocida was isolated from one sample (4.3%), whereas no samples contained Salmonella enterica spp. In eight of the nine samples contaminated with general bacteria, 4.98 to 9.59 log CFU/100 cm2, the amount of bacteria decreased to less than the detection limit after being washed with laundry detergent for a companion animal. As some fabric samples for companion animals were contaminated with a high rate of several species of pathogens, especially P. multocida, which is known to be important in zoonosis, it is necessary to maintain sanitary conditions for the fabric of companion animals.