Bovine parainfluenza virus type 3 (BPIV3) isolates are classified into three genotypes (BPIV3a to c). In 2012, two BPIV3 (HS8 and HS9) strains were isolated from the nasal swabs of cattle kept on two farms in Hiroshima Prefecture. The results of partial genome sequence analysis revealed that the two strains belonged to different genotypes. While the HS8 strain was classified as BPIV3a, the HS9 strain was classified as BPIV3c, which is the latest genotype of BPIV3. This is the first report of BPIV3c isolates in Japan. The low level of the sequence identity of the P gene between the HS9 and BPIV3a isolates suggested that the current RT-PCR method will fail to detect BPIV3c strains. Thus, appropriate primers that can detect all the genotypes need to be designed.
Pronounced rhonchi were observed in a 7.5-year-old Holstein cow. A large mass with fluctuations was also
noted in the buccal region by palpation. An X-ray examination of the head revealed liquid retention in the
maxillary sinus and osteolysis around the root of the upper third molar. These findings suggested that the
mass might be a secondary abscess followed by odontogenic maxillary sinusitis. A necropsy revealed that the
mass was a maxillary sinus abscess. The X-ray examination of the head was useful for the diagnosis of
maxillary sinus abscess.
Anaplasma phagocytophilum is one of the etiological agents of tick-borne rickettsial diseases. It is known as
human and animal granulocytic anaplasmosis. A three-year-old neutered Shih Tzu with no history of overseas
travel became anorexic and feverish one week after being bitten by a tick. The most relevant clinicopathological
findings were thrombocytopenia, neutropenia, and high levels of liver enzyme activity and CRP. The antibody
for A. phagocytophilum was weakly positive in the serum, and PCR analysis confirmed the diagnosis
of A. phagocytophilum infection. Treatment with doxycycline was successful in resolving the clinical signs of
thrombocytopenia and neutropenia. This represents the first documented case of canine A. phagocytophilum
infection to be reported in Japan.
To examine the prevalence of enterohemorrhagic Escherichia coli (EHEC) among cattle transported to a slaughterhouse in Shimane Prefecture, 100 cows raised on 20 farms were surveyed between April and December 2014. As a result of the survey, 10 EHEC O157 strains were isolated from the rectal feces of six cows and the body surfaces of four cows raised on six different farms (A-F) between May and August. Of these strains, nine carried either the stx2a or stx2c gene. In addition, molecular epidemiological analysis was conducted in 2014 using the IS-Printing system, resulting in the isolation of cow and human O157 strains in Shimane Prefecture. These strains showed no identical patterns. However, those isolated from the body surfaces of cows from farms A and B exhibited the same patterns.