The effects of hypochlorous acid water on PEDV were examined. When PEDV was incubated with acidic (pH 3.0‐3.1) or alkaline (pH 11.7‐11.9) hypochlorous acid water at a ratio of 1 : 9 for 1 min, high inactivation rates of hypochlorous acid water (≧99.9%) were observed. In particular, acidic hypochlorous acid water inactivated PEDV (inactivation rate: ≧99.9%) even in the presence of 5% pig fecal matter when incubated for 1 min. Because the cell culture medium (pH 2.7) did not inactivate PEDV, the virucidal effects of acidic hypochlorous acid water did not seem to be attributable to acidity. Hypochlorous acid water has the potential to find widespread use as an alternative to existing disinfectants in stock raising sites, because of its high safety and low environmental footprint.
An 11-year-old castrated male Maine Coon was referred to the Animal Medical Center with a history of no tail carriage. The cat had a loss of perception in the distal part of the tail. Imaging revealed an extradural lesion on the ventral aspect of the L6‐7 region. A L6‐7 hemilaminectomy was performed on the right side. Extruded disk material and conus medullaris were observed in this area. The cat displayed a slight improvement in tail movement, but did not recover perception of the tail. In this case, the conus medullaris was located more cranially than usual, in which the spinal cord ends at the L7 or the sacral vertebrae. Therefore, the neurological signals were limited to the tail. There may be individual differences in the location of conus medullaris in cats.
We investigated the prevalence of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli in cattle and swine at a slaughterhouse in Tottori Prefecture, Japan. ESBL-producing E. coli strains were isolated from the cecal contents in 1 of 93 cattle (1.1%) and 27 of 98 swine (27.6%). The strain isolated from cattle belonged to serotype O6, 10 strains isolated from swine belonged to O143, 2 strains isolated from swine belonged to O25, and others were untypeable. Regarding the ESBL genes of the isolates, 1 isolate from cattle and 22 isolates from swine were classified as the CTX-M-1 group. Of the other isolates from swine, 3 isolates and 2 isolates were classified as CTX-M-2 group and CTX-M-9 group, respectively. As for the antimicrobial susceptibility of the strains isolated from swine, 7 strains, 2 strains and 6 strains were resistant to fluoroquinolones, fosfomycin, and a combination of sulfamethoxazole/trimethoprim, respectively. Regarding the virulence-associated genes of the strains isolated from swine, 1 strain carried both est I and est II, and 1 strain carried est II. The study showed that the prevalence of ESBL-producing E. coli was high in swine of Tottori Prefecture and some strains exhibited multidrug resistance or carried virulence-associated genes.