A number of investigations with regard to correlations usually assumed to exist between hemorrhagic septicemia organisms has hitherto been published, but it is at present yet impossible to differenciate them with distinction, as the results obtained by those who have worked with the organisms are not in agreement.
Employing B. bubalisepticus, B. suisepticus, B. avisepticus and B. bovisepticus I have made some experiments in connection with these problems and came to the following conclusions:
1. Morphology, growth on ordinary culture media, indol production, hemolytic property, bacteriolysis in bile, fermentation test etc. showed no satisfactory evidence as to their identification.
2. No distinction could be obtained from their pathogenicity for mice, rats, guinea-pigs, rabbits and pigeons inoculated or fed with the organisms and also from the postmortem appearances of the experimental animals.
3. Cross agglutination test employing an immune serum obtained from the rabbit inoculated with dead organisms showed that the correlation between the organisms is highly complicated and no specificity could be distinguished in any of them. Agglutinin absorption test gave likewise no satisfactory results, although when employed an immune serum from the guinea-pig inoculated with living organisms the occurence of group reactions was less remarkable and consequently in a certain organisms specificity could be distinguished. Complement fixation test with those sera showed there are so many group reactions that no specificity could be found in any of the organisms. If, however, the complement fixation titres were taken into consideration the organisms could be specified in more or less noticeable degree, but not satisfactory enough for the identfication of them.
4. Active and passive animal protection tests with mice showed in no case so markedly a specificity as to enable us to identify the organisms.
Briefly speaking, it may be assumed that some of hemorrha- gic septicemia organisms may have their specificity, but it is quite impossible to differenciate them by virtue of the specificity which is generally so undistinguished.

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The author carried out bacteriological examination of the vaginal secretion of 200 normal mares, and isolated the following bacteria: the hay bacilli, coli, streptococci and staphylococci. Of these, hay bacilli did not always occur in the standing mare. The percentages of the occurrence of these bacteria as they had been isolated on the common agar were: 84.5% with the coli, 71.5% with the streptococci, 62.0% with the hay balilli and 28.0% with the staphylococci. The streptococci occurred less frequently in the young mares. Both the hemolytic and the nonhemolytic groups of them were isolated, the former was still further identified to belong to either one or the other of the βm, βg and β+ types. The staphlococci consisted of three species, i. e. aureus, albus and orangeus. By saccharolytic test with the 30 strains, isolated, 15 were pathogenic. From 3 cases out of the total 200 mares examined, B. pyocyaneus was isolated.

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In recent years, especially in summer time, infectious pneumonia has been very prevalent among lambs of the Government Sheep Farm at Tomobe (Prefecture Ibaraki), causing great losses every year. The results of our investigation on the disease are summarized as follows:
At post-mortem of animals dead from the disease were demonstrated severe anaemia owing to numerous nematodes in the stomach and intestine, pneumonia catarrhalis purulenta lobaris (part of middle lobe, and outer half of anterior and posterior lobes affected) with pleuritis fibroplastica. The histological examination of the affected part of lung revealed hyperaemia, desquamation of alveolar epithelium, exudation of serous fluid, large mononuclear cells and leucocytes in alveoli, leucocytic and lymphocytic infiltration as well as connective tissue production around the bronchioli.
From the affected areas of the lung were isolated Bacillus pyogenes, Bacillus ovisepticus, a small Gram-positive bacillus, Micrococcus catarrhalis, a streptococcus, and a staphylccoccus, of which the first three organisms are to be considered most significant for the disease in view of high percentage of their occurrence in pulmonary lesions, their great virulence for the lamb and laboratory animals, and of intimate immunological relations between these organisms and the serum of affected lambs.
As the mixed vaccine prepared from these three organisms gave a good result in laboratory animals and a few lambs treated, double treatments with the vaccine were practised this year on the Government Sheep Farm where the administration of vermifuges was enforced at the same time. As the result of the prophylactic measures cases of the disease and losses to the Farm were less than one-tenth those in the previous years.

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In the previous number of this journal (Vol. III, No. 3), Matsuba, one of the authors, published the first part of this experiment under the article "On the effect of castration upon the thyroid gland, hypophysis, thymus and adrenal in the albino rat." This paper is the second part of the same experiment, dealing with the growth of various organs and the effect of castration upon them excluding endocrine organs.
The material used included 300 rats of male sex, varying from newborn to 300 days of age. The 300 rats were all raised up in our laboratory and divided into 500 lots according to litter and age. One to three rats from each lot which consisted of three to eight rats were fed as controls.
All the rats excepting the controls were castrated on the 15th, 30th, 40th, 50th, 60th, 75th, 90th, 120th, and 150th day of age and killed with controls at an interval of about 30 days, extending from 60 days to 300 days of age.
During the course of experiment all the rats were weighed every other day and the organs to be examined were obtained immediately after the animal were killed and then precisely weighed.
The results obtained from the experiment can be summarized as follows.
1. The post-natal growth of the castrated rats was in a fair agreement with that of the controls. No remarkable increase was observed in the body weight of the castrated animals; the fatty condition of the animals employed, possibly due to lack of proper feeding and management, might have caused no difference in the body weight of the castrated and the centrols.
2. No effect of castration was observed in the body weight of rats, but the tail length of the castrated animals increased by 5% on an average.
3. The hearts of the castrated animals develop as good as those of the controls before 180 days of age, but hereafter they showed decrease in their development. A decrease has also been found in the growth of hearts of animals castrated in younger age than those operated in advanced age.
4. The weight of kidneys was less by 20-30% in the castrated than in the controls. In the weight of liver no effect of castration was observed. The spleen of the operated was all heavier than that of the control during a certain course of time after castration, but in the operated animals a decrease was found in its weight after 200 days of age.
5. The effect of castration upon the length of long bones was quite remarkable; they have been found especially shorter in the animals castrated before 60 days of age. As in the case of the length, the weight of the long bones was also much less in the animals operaied before 60 days of age.
6. The better was the deposit of calcium in the bone, the later the castration was performed. As regards the P and Mg contents in the bone no difference has been constated between the castrated and the controls.
7. The resistance of long bones against the artificial fracture seemed to decrease due to castration. The femor was weaker in the animals operated after 60 days of age and the tibia in those castrated before the age mentioned.
8, The castrated animals were generally stronger in pulling weight. The inferiority of the pulling power in the controls may probably due to their unrestless behaviour.

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