[in Japanese]

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[in Japanese]

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All six virus which had beern isolated from the feces of apparently healthy cattle weretested for properties after serial passages. Then growth curves were drawn for them, includingtwo prototype viruses, in cells of bovine kidney, chick embryo, HeLa, PS (cell line isolatedfrom swine kidney), and TC (cell line isolated from chick embryo).I) All strains proliferated xxziLh cytopathogenic effect irr cultures of bovine kidney andPS cells. They were divided ixnto txxzo groups in cultures of HeLa cells. One group, including the K62 and K90 strains, grew with cytopathogenic effect and the other groupshovved neitlner virus multiplication nor cytopathogenic effect in HeLa cells. The K62, K88, K90, and K130 strains exhibited virus growth in TC cells, where the K136 and K145strains did not proliferate.2) 1-Iernagglutinating activity was changed in the serial passages in the cells used withbovine enteroviruses. Fluids of tissue cultures of the K62 and K90 strains in HeLa cellsand the K130 strain in TC cells failed to produce hernaggltatinating activities with two orthree passages. The K136 strain in PS cells and the K145 strain irn chick embryo cells, however, produced hemagglutinating activities with two or three passages. The K130 strainproduced no clear-cut hemagglutination by successive passages in chick embryo cells. Theother strains failed to change the hemagglutinating activity with serial passages itn TC cells.The pltenornenon has been recognized not only with human enteroviruses but also withbovine enteroviruses. It has been manifested not only by the virus which passed in malignantcells but also by the viruses which passed in normal heteroTogous cells.3) The progeny of the viruses began to be produced in bovine kidney cells 6 to 8hours after infection. Thereafter infective titers increased frc>rn 18 to 24 hours and reachedthe highest values at 24 to 36 hours. Virus growth occurred in PS and HeLa cells withinthe same time as in bovine kidney cells and earlier than it? chiclc embryo cells, in whichthe highest L

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[in Japanese]

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It has been well known that most of the acetyl choline (ACh) in the brain tissue is ininactive bound form, while a considerable amount of ACh is actually found in free form.It seems that some free ACh is released artificially from bound ACh during estimation.Recently it has been reported that most part of the bound ACh in the brain was distributedin the particulate fraction. It still remains obscure, however, whether ACh is incorporatedsimply in particles or bound chemically with them. It seems to be an effective aproachto these problems to develop an inproved estimating method with less artificial effect tostudy ACh release from particles in vitro. This paper deals with a method for estimationof free and bound ACh. It also describes the effects of some inorganic ions on the rateof ACh release from the brain particulate fraction in vitro. The results obtained aresummarized as follows. The rate of ACh release was lower in a O.32N1 sucrose solutioncontaining 5xlO M eserine salicyte at OC than in any other solution tested, i.e., acidalcohol or frog Ringers solution. The free ACh in the rabbit whole brain was assayeddirectly with the homogenate of the brain, using the rectus abdominis muscle. As a resttlt, it was about 30% of total ACh. A hypertonic sucrose solution had only a small effect onthe rate of ACh release, as well as an isotonic sucrose solution, while a hypotonic sucrosesolution had an increasing effect on it. A Nail solution showed the same effect oftonicity on the rate of ACh release as the sucrose solution. Within a range of 0.32 to 0.62in tonicity, potassium ion in a high concentration had an increasing effect on the rate of AChrelease, while calciurn and magnesium tons ltad a small or no and sodium Ton no effect on it. Allthese tons, even potassium ion, had no effect in Ringers type concentratiorn. From theseresults, it might be assumed that ACh was not simply incorporated in particles but was bound to them, and that it was released from them by dilution and the presence ofpotassium ion.

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