The Japanese Journal of Veterinary Science
Online ISSN : 1881-1442
Print ISSN : 0021-5295
ISSN-L : 0021-5295
Volume 39, Issue 2
Displaying 1-14 of 14 articles from this issue
  • Toshihiko NAKAO
    1977 Volume 39 Issue 2 Pages 93-100
    Published: April 25, 1977
    Released on J-STAGE: February 13, 2008
    JOURNAL FREE ACCESS
    Effects of synthetic glucocorticoids and ACTH on the adrenal cortex were studied in 4 cases of cystic ovarian disease (COD) involving 3 cows. Of 2 cases showing anestrus, one was injected intramuscularly (i.m.) with 20mg of betamethasone (Bet) daily for 5 days and the other with 10mg of Bet daily for 4 days. Of the other 2 cases exhibiting symptoms of true nymphomania, one was injected i.m. with 20 mg of Bet daily for 5 days and the other with 10 mg of dexamethasone daily for 4 days. Before and after successive administration with glucocorticoids, 50IU of ACTH was injected i.m. in the 3 cases. The serum concentrations of 11-hydroxycorticosteroids (11-OHCS), progesterone, and proteins were determined daily throughout the experimental period. The response of serum 11-OHCS to ACTH was examined 30 minutes after injection. Glucocorticoids inhibited the release of adrenal corticosteroids in all the 4 cases. A fall of the serum 11-OHCS level was shown earlier after injection with 20 mg of glucocorticoids than after that with 10 mg. Following the termination of injection with glucocorticoids, the serum 11-OHCS level increased remarkably in the 2 cases showing anestrus, but did not in the 2 cases exhibiting true nymphomania. The response of serum 11-OHCS to ACTH was lower in the cows with COD than in control cows with a normal estrous cycle. The serum progesterone level presented essentially the same pattern of fluctuation in response to glucocorticoids and ACTH as the serum 11-OHCS level. Serum proteins decreased slightly after the 4th day of injection with glucocorticoids. From these results, it is suggested that adrenal dysfunction may be related to the different appearence of estrous abnormalities in cows with COD.
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  • Yoichi OGHISO, Yong-Soon LEE, Reiji TAKAHASHI, Kosaku FUJIWARA, Kenya ...
    1977 Volume 39 Issue 2 Pages 101-109,116
    Published: April 25, 1977
    Released on J-STAGE: February 13, 2008
    JOURNAL FREE ACCESS
    Histopathological examination was made on affected skeletal muscles from 10 cases of spontaneous eosinophilic myositis in Japanese Black cattle. Prominent regressive lesions with increased connective or adipose tissues were observed in affected skeletal muscles of all cases and also in the tongue, diaphragm and myocardium of a few cases. Small arteries or arterioles in muscular lesions showed edematous swelling of the intima, degeneration of the media and cellular infiltration. Infiltration of eosinophils, lymphocytes and plasma cells was remarkable with some neutrophils, while neither basophils nor mast cells appeared. Interestingly, degranulation of infiltrated eosinophils was encountered frequently accompanying eosinophilia in local lymph nodes, suggesting allergic basis in pathogenesis of the disease. Neither parasites, common bacteria, mycoplasmas nor viruses were detected, while a few sarcosporidial cysts without any reactions were seen in muscle fibers of 4 cases.
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  • Shin-ichiro KONISHI, Ryo HARASAWA, Masami MOCHIZUKI, Hiroomi AKASHI, M ...
    1977 Volume 39 Issue 2 Pages 117-125
    Published: April 25, 1977
    Released on J-STAGE: February 13, 2008
    JOURNAL FREE ACCESS
    Two cytopathogenic agents were isolated in horse kidney cell cultures from the bronchus and lung of a three-month-old Thoroughbred colt with pneumonia presumed to be caused by bacterial infection. They were propagated in equine fetal dermis cells with cytopathogenic effect and development of intranuclear inclusions similar to those in primary horse kidney cell cultures. The isolate from the pneumonic lesion was used as a representatiVe for characterization, because both isolates were identical in the cross serum neutralization (SN) test. The isolate was found to contain DNA because of its replication inhibited by 5-iodo-2'-deoxyuridine. Its buoyant density was 1.29 g/cm3. The virion had a cubic symmetry without envelope, and measured approximately 74 nm in diameter. The virus was resistant to chloroform and ether. It was slightly inactivated at pH 3.0 for 3 hours. Hemagglutination was observed in human (type O), guinea pig, horse and rat erythrocytes. The equine isolate shared a common complement-fixing antigen with a canine adenovirus, but it did not react with this virus in the SN test. A serologic survey on a stud from which the foal in the present study was derived demonstrated a prevalence of adenoviral infection.
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  • Hiroshi OIKAWA, Harumoto KAWAGUCHI, Kozi NAKAMOTO, Kiyoshi TSUNODA
    1977 Volume 39 Issue 2 Pages 127-134
    Published: April 25, 1977
    Released on J-STAGE: February 13, 2008
    JOURNAL FREE ACCESS
    F'ield surveys on coccidial infection in Japdnese broilers were performed with 4, 454 fecal samples in 1974 and 1975. The Eimerian species were identified by a combination of two methods, examination of oocyst morphology and chicken passage test. Oocysts were detected from 57.5% of 1, 896 samples in 1974 and from 69.8% of 2, 558 samples in 1975. Eimeria acervulina was found in 98.1 and 98.9% of the oocyst-positive samples, and E. maxima was found in 11.8 and 10.1% in 1974 and 1975 respectively. The Tenella type oocysts were found in 40.5 and 28.3% of the oocyst-positive samples in 1974 and 1975, respectively. In the chicken passage test of the Tenella type oocysts collected in 1975, E. tenella, E. necatrix and E. praecox were found in 69.8, 9.3 and 74.4%, respectively, in the form of single or mixed infection with two or three species. Annual, seasonal, regional and age differences in oocyst detection rate from 1973 to 1975 were significant at the 1% level. The annual and seasonal differences are considered to be due to the effect of coccidiostats used as one source of variances. Oocyst detection rate increased up to 40 days of age and kept plateau thereafter. E. acervulina oocysts were found at anextremely high rate in all the ages. Oocysts of the Tenella type and E. maxima were found rather low rate in younger ages up to 30 days.
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  • Yasuhiko KANO, Toru SAWASAKI, Mifune HIRAYAMA, Kuniyasu IMAI
    1977 Volume 39 Issue 2 Pages 135-140
    Published: April 25, 1977
    Released on J-STAGE: February 13, 2008
    JOURNAL FREE ACCESS
    A study was carried out to determine whether acclimation to a certain environmental temperature in early life had any effect on lipid metabolism in later life at a different environmental temperature. Young mice were acclimated to either low or high temperature and exposed to either warm or cold environment. After a certain period of acclimation or exposure, the serum lipid contents were determined. The serum lipid contents varied with changes in environmental temperature. After prolonged exposure, regardless of the history of animals, they were affected with the environmental temperature at which the animals were placed. Acclimation in early life had no aftereffect on later life at a different environmental temperature.
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  • Takeo MIZUTANI
    1977 Volume 39 Issue 2 Pages 141-147
    Published: April 25, 1977
    Released on J-STAGE: February 13, 2008
    JOURNAL FREE ACCESS
    Massive and persistent obesity was induced in mice by a single intracerebral injection with 4-nitroquinoline 1-oxide (NQO). It was characterized by determining the effect of strains of mice or vehicles of NQO on the development of obesity, and by comparing the body composition, organ weight, and glucose tolerance between NQO-obese and control mice. Treatment with NQO resulted in a higher incidence of obesity in C57BL/6 mice than in ddOM, C3Hf and DBA/2 mice. A much higher obesity-inducing rate was also given by treatment with NQO prepared in 10% lecithin than in 0.5% Tween 80 or 30% dimethyl sulfoxide. The weights of the liver and heart, as well as water, protein and fat contents of the body, were significantly larger in NQO-obese mice than in controls. NQO-obese C57BL/6 mice showed to fall transiently in a state of chemical diabetes 12 weeks after treatment with NQO.
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  • Katsuya ISHIHARA, Yasuji SUGANUMA, Yukio WATANABE
    1977 Volume 39 Issue 2 Pages 149-157
    Published: April 25, 1977
    Released on J-STAGE: February 13, 2008
    JOURNAL FREE ACCESS
    The present studies were undertaken to obtain data on serum lipoprotein analysis which would serve as normal references for studies on serum lipoproteins in diseased dogs, especially those with canine dirofilariasis. Serum samples were collected from 35 normal dogs of various ages proved to be free from Dirofilaria immitis (filaria) by the routine physical checkup and laboratory examination. They were analyzed for lipoproteins by cellulose acetate electrophoresis (electrophoresis). The serum lipoproteins were obtained basically as four distinct fractions, i.e., α-, pre-β-, β-lipoprotein and chylomicron fractions. The last-named fraction was only in trace quantities. It was presumed that the four fractions might have a density of 1.063<1.210, 1.006<1.019, 1.019<1.063 and <1.006, resptctively, as estimated from the electromigration velocities (mobilities) of other samples which had been separated ultracentrifugally. The mobility of the pre-β-lipoprotein fraction, however, varied with the serum sample, possibly due to the difference in the quantity of lipids bound to this fraction of serum lipoproteins. On the basis of analytical data on the 35 normal dogs, the normal percentage composition (relative proportion) of the serum lipoprotein fractions was investigated in dogs. Furthermore, the concentrations of these fractions in the serum were calculated from data on the total serum lipid content, the percentage composition of the serum lipoprotein fractions, and the amounts of lipids bound to the lipoprotein fractions obtained from 25 of the 35 dogs in order to clarify the normal ranges.
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  • Yu IDE, Hajime MATSUMOTO
    1977 Volume 39 Issue 2 Pages 159-164
    Published: April 25, 1977
    Released on J-STAGE: February 13, 2008
    JOURNAL FREE ACCESS
    When goats were administered orally with urea in a dose of 0.3 g per kg of body weight, the blood concentration of glutamine-N in the jugular vein increased to about 6μg per ml of plasma, reflecting the removal of ammonia by forming glutamine in the animal body. The blood glutamine seemed to undergo a more rapid turnover than the blood urea. Glutamine synthetase catalizes the formation of glutamine from ammonia and glutamic acid. It was made clear that the activity of the enzyme per gram of tissue was the highest in the spleen, which was followed by the retina, cerebrum, liver, cerebellum and kidney in this regard. The whole tissue activity of the enzyme, however, was higher in the liver than in the spleen. It was considered that the activity of glutamine synthetase in the spleen increased in kids with growth, and that the spleen might play an important role in forming glutamine in adult goats.
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  • Yoshihiro HAYASHI, Takao NISHIDA, Koshi MOCHIZUKI, Sueshige SETA
    1977 Volume 39 Issue 2 Pages 165-174
    Published: April 25, 1977
    Released on J-STAGE: February 13, 2008
    JOURNAL FREE ACCESS
    The purpose of this study was to develop and analyze methods for determination of age and sex for the Japanese wild boar (Sus scrofa leucomystax). The following results were obtained: (1) Eighty-nine skulls of wild boar were divided into five age groups on the basis of tooth eruption and wear, (2) the annual layers in dentin and cement of the first lower molar permitted the recognition of five age groups separated by one year (7-8, 19-20, 31-32, 43-44, 55- month), (3) an annual layer consisted of two bands, one broad and one narrow, and it was shown that the latter was hypercalcified, (4) no significant difference could be demonstrated between the tooth eruption of known age captives and that of wild boars, and (5) of three sex criteria examined in this study, canine root was the best character for sex determination, especially for younger animals.
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  • Kosuke OKADA, Yutaka FUJIMOTO
    1977 Volume 39 Issue 2 Pages 175-177,179
    Published: April 25, 1977
    Released on J-STAGE: February 13, 2008
    JOURNAL FREE ACCESS
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  • Junzo SAEGUSA, Katsumoto UEDA, Yoshitaka GOTO, Kosaku FUJIWARA
    1977 Volume 39 Issue 2 Pages 181-185
    Published: April 25, 1977
    Released on J-STAGE: February 13, 2008
    JOURNAL FREE ACCESS
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  • Yoshimitsu MAEDE
    1977 Volume 39 Issue 2 Pages 187-189
    Published: April 25, 1977
    Released on J-STAGE: February 13, 2008
    JOURNAL FREE ACCESS
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  • Masami MOCHIZUIKI, Shin-ichiro KONISHI, Manabu OGATA
    1977 Volume 39 Issue 2 Pages 191-194
    Published: April 25, 1977
    Released on J-STAGE: February 13, 2008
    JOURNAL FREE ACCESS
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  • Kaoru KOSHIMIZU, Teruo MAGARIBUCHI
    1977 Volume 39 Issue 2 Pages 195-197,199
    Published: April 25, 1977
    Released on J-STAGE: February 13, 2008
    JOURNAL FREE ACCESS
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