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Volume 42, Issue 1
Displaying 1-13 of 13 articles from this issue
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Toshio HONMA, Misao ONUMA, Takeshi MIKAMI, Hisao IZAWA1980 Volume 42 Issue 1 Pages 1-4
Published: February 25, 1980
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESSAs a new method for the detection of bovine leukemia virus (BLV) infection, syncytium assay was evaluated with lymphocytes from infected cattle and sheep. Only when lymphocytes from cattle with enzootic bovine leukosis and from sheep experimentally infected with BLV were used, syncytium formation was induced among indicator cells. A direct proportional dose response was presented by the number of inoculated lymphocytes from BLV-infected cattle to the number of syncytia formed. Treatment of lymphocytes from cattle with an adult form of lymphosarcoma with anti-BLV serum could inhibit the syncytium formation, but neither antiserum against bovine syncytial virus nor the sera from cattle with sporadic form of lymphosarcoma could inhibit. These results indicate the specificity and the usefulness of this assay for the detection of BLV infection.View full abstractDownload PDF (465K) -
Toshio HONMA, Misao ONUMA, Takeshi MIKAMI, Hisao IZAWA1980 Volume 42 Issue 1 Pages 5-8
Published: February 25, 1980
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESSThe presence of antibody against bovine leukemia virus (BLV) was examined in dairy and beef cattle in Hokkaido and in the Towada district, Aomori Prefecture, by the immunodiffusion test in 1977 and in 1978, respectively. The percentage of antibody-positive cattle was higher in those cattle than in those observed in the previous survey (8.8% vs. 3.3% in Hokkaido and 44.2% vs. 32.2% in the Towada district), indicating the gradual increase of BLV infection in these parts of Japan. To clarify a relationhsip between the existence of BLV and the presence of antibody against BLV in cattle, syncytium assay (SA) was performed with lymphocytes from cattle with or without BLV antibody. All the cattle with antibody against BLV were positive for SA when bovine splenic cells were used as indicators, whereas 71.4% of them was positive when bovine thymic cells were used as indicators. These results suggest that BLV may be detetced by SA from nearly all the cattle with antibody against BLV when appropriate cells are used as indicators. Fifteen percent of the antibody-negative cattle was positive for SA when bovine thymic cells were used and 17.6% positive when bovine splenic cells were used. Neither plaque nor inclusion body formation was observed in indicator cells which had been inoculated with lymphocytes. These SA-positive, antibody-negative cattle were considered to have been infected with BLV.View full abstractDownload PDF (531K) -
Hiroshi NAGATOMO, Hitoshi KOTANI, Manabu OGATA, Takamasa SHIMIZU1980 Volume 42 Issue 1 Pages 9-15,17
Published: February 25, 1980
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESSAgar-gel precipitation tests were carried out for serological studies of bovine ureaplasmas isolated from pneumonic calf lungs and urogenital tracts. A common precipitation line was observed between antigens and antisera of the representative bovine strains of serogroups previously determined by the metabolism inhibition test. The common antigen of the bovine ureaplasmas was also shared with Ureaplasma strains of human, feline, caprine, canine and fowl origins. It was never detected in 12 species of mycoplasmas and 2 species of acholeplasmas in the gel precipitation test. The results of the investigation suggest that the genus Ureaplasma may have an antigenic component common to the strains isolated from human and various animal species, but that it may be distinct from the strains of Mycoplasma and Acholeplasma.View full abstractDownload PDF (914K) -
Yoshiharu HASHIMOTO, Makoto SUGIMURA1980 Volume 42 Issue 1 Pages 19-25,29
Published: February 25, 1980
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESSDistribution and morphology of anti-horseradish peroxidase antibody-producing cells (APC) of ducks were studied by light and electron microscopic immunocytology. The typical APC were plasma cells and large lymphoid cells in splenic red pulp or germinal centers. In the germinal center region, two types of positive cells were identified; the predominant positive cells in quantity were dendritic cells (DC), and the other was typical APC. In the course of cell appearance, these typical APC followed that of extragerminal center region of spleen. Other lymphoid cells in the splenic white pulp; the periarterial, the perivenous and the periellipsoidal lymphoid cells showed no evidence of antibody-production. These results suggest that some of the APC of ducks originate in the germinal centers during the course of response, and germinal centers of duck spleens and lymph nodes functions as an indisprensable lymphatic element in their antibody-producing system.View full abstractDownload PDF (2207K) -
Hitoshi KOTANI, Hiroshi NAGATOMO, Manabu OGATA1980 Volume 42 Issue 1 Pages 31-40
Published: February 25, 1980
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESSCultures for Ureaplasma isolation were made from the oral cavity, nasal cavity, eye, rectum, prepuce and vagina of 62 goats of 3 separate herds and 11 sheep of one herd. All the animals were apparently healthy. Ureaplasmas were obtained from the prepuce (6/16, 37.5%) and vagina (18/46, 39.1%) of goats and from the prepuce (1/5, 20%) and vagina (2/6, 33.3%) of sheep, but not from any other organ. The caprine and ovine Ureaplasma strains were examined serologically by the growth inhibition and metabolism inhibition tests. Twenty-three caprine strains formed a single serological group, and 21 ovine strains were divided serologically into twenty forming a group and one distinct from these strains. Moreover, 2 groups of Ureaplasma strains isolated from the goats and sheep, but one ovine strain, were found to have a common antigen. Neither caprine nor ovine strains had any serological relationship with human, bovine, canine, simian or avian strains. The fact that the caprine and ovine strains have an antigenic similarity suggests that the host-specificity of Ureaplasma may be an exception for the caprine and ovine strains.View full abstractDownload PDF (835K) -
Takamasa SHIMIZU, Takeshi YAGIHASHI1980 Volume 42 Issue 1 Pages 41-47
Published: February 25, 1980
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESSSerological and biochemical studies were performed with 86 Mycoplasma strains isolated from airsacculitis, trachea, eye cavity, oropharynx and cloaca of a total of 50 turkeys raised in two farms in Miyazaki. In addition, 4 strains originating from the oropharynxes of 2 out of 3 turkeys raised in a farm in Tokyo and affected with airsacculitis were examined. Forty-six strains from Miyazaki and one strain from Tokyo were identified as Mycoplasma meleagridis. The remainder, 43 strains, was serologically identified as M. gallinarum (4 strains), serogroup D (5), serogroup I (18) and unidentified (16). Biochemically, M. meleagridis was characterized by being positive for the production of phosphatase and breakdown of arginine. Contamination with M. meleagridis among turkeys reared in many parts of Japan is suggested.View full abstractDownload PDF (1187K) -
Yasunobu EGUCHI, Satoshi HANADA, Yoshio MORIKAWA1980 Volume 42 Issue 1 Pages 49-59
Published: February 25, 1980
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESSElectron microscopic examination was made on the cells of the anterior pituitaries in normal or castrated late fetal and early neonatal male rats. It was posbible to distinguish three types of granular cells in addition to celld of uncertain identity, on the basis of the diameters of granules. Type I cells had granules with a small range of diameters (40-180nm). Secretory granules of these cells were located mainly in the periphery of the cytoplasm. The cisternae of rough endoplasmic reticulum (RER) were flattened and irregularly arranged. Type II cells had somewhat larger granules (40-200nm) than type I cells. Granules of type II cells were located throughout the cytoplasm. The RER cisternae appeared as vesicular or short plates, or were grouped as lamellar stacks. Type III cells had granules with the widest range of diameters (90-360nm). Following castration, both type I cells and type III cells showed little cytological changes, but type II cells exhibited extreme changes accompanied by a marked enlargement of the RER cisternae and the Golgi complex. Such changes in type II cells were more marked in fetused than in neonatal rats, and were prevented by an injection of testosterone propionate. These observations support the view that type II cells include gonadotrophs and that the pituitary gonadotrophic activity declines just after birth in male rats.View full abstractDownload PDF (2047K) -
Masamichi KUROHMARU, Takao NISHIDA, Koshi MOCHIZUKI1980 Volume 42 Issue 1 Pages 61-67,71
Published: February 25, 1980
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESSThe intestinal tract of the musk shrew, Suncus murinus (family Soricidae, order Insectivora) was morphologically studied by light and electron microscopy. Macroscopically, the intestine was characteristically short out of proportion to the body length of this animal and was devoid of the cecum, making a demarcation between the small and the large intestines obscure. Not only the small intestine but also the part equivalent to the large intestine was studded with villi on the mucosal surface. Each villus did not have any developed creases, but had several scattering mucus-releasing goblet cells on its lateral surface. Although Paneth cells were absent in the intestinal glands, basal-granulated cells were often observed, especially in the rectal portion. The structure of absorptive epithelial cells of this animal was almost similar to that of other mammals and was also almost the same in any part of the intestine.View full abstractDownload PDF (1241K) -
Masato KUWABARA, Masaki KOBAYASHI, Kei SATO1980 Volume 42 Issue 1 Pages 73-81
Published: February 25, 1980
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESSIn order to study patellar luxation (PL) in dogs, analysis was made on 83 silhouettes of femora collected from dogs with normal gaits and 5 silhouettes of femora collected from dogs suffering from PL. The silhouettes of the distal end of the femur were classified according to the factor scores. Computer analysis showed the following results. A) Puppies from 80 to 110 days old revealed silhouettes similar to those of the femoral distal end of an adult dog. On the other hand, puppies about 50 days old revealed silhouettes similar to those of PL, as shown in Fig.4. B) The results of the computations indicated the difference between male and female. More clearly, they were divided into three groups by the shape of the silhouettes of the femoral distal end according to the stage of postnatal development. (1) In the first group, the distal end of the femur developed completely. All adult males belonged to this group, as shown in Fig.5. (2) In the second group, the distal end of the femur did not completely develop. All adult females and some adult males and puppies belonged to this group, as shown in Fig.5. (3) In the third group, the patellar surface and the lateral condyle of the femoral distal end developed incompletely. Both puppies less than 80 days old and adult dogs suffering from PL belonged to this group, as shown in Fig.5.View full abstractDownload PDF (789K) -
Hiroyasu EJIMA, Kazuo KUROKAWA, Tsuneo ABE, Shigenori IKEMOTO1980 Volume 42 Issue 1 Pages 83-88
Published: February 25, 1980
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESSStudies were made to clarify the specificities of anti-lymphocyte antibodies produced by allo-skin transplantation and pregnancy in bitches. Nine cytotoxic antibodies, designated anti DLA-J1, -J2, -J3, -J4, -J5, -J6, -J7, -J8 and -J9, were obtained from eight of 17 sibling beagles after allo-skin transplantation and from a pregnant bitch. A two-stage microlymphocytotoxicity technique was used for the detection of antibody. The antibody titer was expressed as the reciprocal of the final serum dilution causing 30% cytotoxicity for skin-donor lymphocytes and the sire's lymphocytes. It ranged from 4 to 512. From the results of the serogram and X2 values determined by the screening test with 40 dog lymphocytes, the antibodies obtained were examined. Because the X2 values were 6.2, 4.0 and 7.3 in anti DLA-J1-J2, -J1-J8, -J2-J8, and 4.8 in anti DLA-J3-J6, it was presumed that anti DLA-J1 might be practically identical with anti DLA-J2 and -J8, and anti DLA-J3 with anti DLA-J6. The specificities of the nine antibodies obtained were classified into six kinds; that is, anti DLA-J1-J2-J8, -J3-J6, -J4, -J5, -J7 and -J9.View full abstractDownload PDF (644K) -
Jit SINGH, D. C. DHABLANIA, B. PRASAD, S. S. RATHOR1980 Volume 42 Issue 1 Pages 89-91
Published: February 25, 1980
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESS -
Toshiho NISHITA, Tokihiro MATSUZAWA1980 Volume 42 Issue 1 Pages 93-95,97
Published: February 25, 1980
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESS -
Takayuki MURAKAMI, Isao SAITO, Koshi MOCHIZUKI1980 Volume 42 Issue 1 Pages 99-101
Published: February 25, 1980
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESS
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