Line censuses have been undertaken at the Imperial Palace, Tokyo, to reveal the avifauna, since 1965. Here we report results of the line census from April 1976 to March 1996. The line census was conducted on a route of 4.1 km between 9:00 – 12:00, in the same way as the census conducted continuously before and after this study. We divided the study period into the early one from April 1976 to March 1986 and the late one from April 1986 to March 1996, and compared the two study periods. We observed 23,190 individuals of 76 species in the early study period (68 censuses) and 29,887 individuals of 72 species in the late study period (95 censuses; 89 species through the whole study period). Comparing 14 species with the higher relative frequency in 10-year-period before the early study period, and the 4-year-period after the late study period as well as the two study periods, Aix galericulata, Anas zonorhyncha, Streptopelia orientalis, Nycticorax nycticorax, and Spodiopsar cineraceus seemed to decrease, while Yungipicus kizuki, Sittiparus varius, Parus cinereus, Hypsipetes amaurotis, Zosterops japonicus, and Emberiza personata seemed to increase. Among these species, Aix galericulata, Anas zonorhyncha, Streptopelia orientalis, Nycticorax nycticorax, Yungipicus kizuki, Parus cinereus, Zosterops japonicus, Spodiopsar cineraceus, and Emberiza personata fluctuated significantly between the early and late study periods. Five and six species without the higher relative frequency also significantly decreased and increased, respectively. For Nycticorax nycticorax, Cyanopica cyanus, Zosterops japonicus, and Spodiopsar cineraceus, in which the numbers observed changed significantly between the two study periods, the changes seemed related to seasonal change. Conversely, the other species in which the observed number changed significantly, did not seem to change seasonally but to increase or decrease through a year. From December to March, ca. 25 species were observed. Twenty seven and twenty five species were observed in April in the early and late study periods, respectively; thereafter the number of species declined and reached a minimum of 17 and 15 species in August, the early and late study periods, respectively, and then switched to an increase during September and November. In November, the number of species reached 23 and 26, respectively. However, the number of species did not differ significantly in any month between the two periods.
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