Considering the possible change in biological properties associated with the colonial dissociation of Pseudomonas aeruginosa reported by Shionoya and Homma, broth cultures obtainedfrom stock cultures on nutrient agar slants originated from a “la colony” of each test-strain were used throughoutthis study. Pyocine typing was performed in all the cases using Tryptosoy agar plates containing 5% bovine blood (s. the first report; Vol.45 No. 10 of this Journal).
The results of typing showed reproducibility in 98 out of 121 strains by repeating the typingtwice or more. The results of the 121 strains were: 46 strains of Type 1, 21 of Type 3, 10 of Type10, 7 of non-producer, three strains each of Types 22 and 29, two each of Types 5 and 33, and oneeach of Type 16 and of three types not described by Gillies and Govan. Six strains ofthe remainder were classified under the tentatively named “Type 1/10”, and 17 were “variable type”.
The cross-reactions using 29 strains of Type 1 and 10 strains of Type 10 each other are shown in Tables 1-4 separately. From Tables 1 and 2, both the types were regarded as to be subtypable each by applying some appropriate strains as additional indicators. As shown in Tables 3 and 4, eight strains, the second group in Table 1, did not react to 7 strains of Type 10. This finding suggests that there exists some deep relationship between a part of Type 1 strains and most ofType 10 strains
Sixteen Type 1 strains isolated from 6 patients were studied for theiridentity bycross-expreiments. As shown in Table 5, most of strains isolated from apatient exhibited the same inhibition and sensitivity patterns, and thoat different patterns were observed in allthe strains obtained from other patients. It can be said therefore that cross-test may be serviceable in differentiatingstrains of the same pyocine type. in view of bacterial genetics.
According to the cross-experiments using 24 strains of Type 3 (Table 6), some cross-reactions were recognizable among them too. It appeared possible that this type may be also possible to be subtyped by applying some additional indicators. In contrast, as shown in a part ofTable7, there were no crossreactions to be observed among each of 2-4 strains of Types 5, 22, 29 and 31. In view ofthe results in this table which concern with each four strains of Types 1, 3 and 10 in addition, it can besaid that “immune phenomenon” is not to be observed at all between any two types tested here.

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In December 1966, the test of hemagglutination-inhibition (HI) against the Nakayama-RFVL strain of Japanese encephalitis virus (JEV) was carried out on 507 healthy adults living in Fukuoka City and its environs.
Two hundred and twenty out of 507 serum samples (43.4%) were HI positive (titers of 1: 10 or more). The positive rates increased with age. The HI titers remained nearly stationary after six months, and it was estimated that the HI antibody acquired by subclinical infection was stable.
Two hundred and twenty-four HI negative persons were vaccinated by two subcutaneous injections of 1.0ml of JEV vaccine at weekly intervals. They were bled a month after the first inoculation, and 178 out of the 224 cases (79.5%) changed to HI positive.
In the vaccinated cases, 40 serum specimens which acquired comparatively high antibody titers after the vaccination were examined for 2-mercaptoethanol sensitivity, and it was found that all of the samples were resistant to the reagent.

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