Kansenshogaku Zasshi
Online ISSN : 1884-569X
Print ISSN : 0387-5911
ISSN-L : 0387-5911
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Volume 45, Issue 9
Displaying 1-7 of 7 articles from this issue
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  • [title in Japanese]
    [in Japanese]
    1971 Volume 45 Issue 9 Pages 375-384
    Published: September 20, 1971
    Released on J-STAGE: September 07, 2011
    DOIhttps://doi.org/10.11150/kansenshogakuzasshi1970.45.375
    JOURNAL FREE ACCESS
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  • Studies on Hemolytic Factors of Vibrio parahemolyticus
    I. Hemolytic Activity of the Supernatant Fluid of its Broth Culture
    Yasushi OBARA
    1971 Volume 45 Issue 9 Pages 385-391
    Published: September 20, 1971
    Released on J-STAGE: September 07, 2011
    DOIhttps://doi.org/10.11150/kansenshogakuzasshi1970.45.385
    JOURNAL FREE ACCESS
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    It is well known that most strains of Vibrio parahaemolyticus originated from fuman patients demonstrate on a specially prepared blood agar plate a hemolytic activity called Kanagawa-phenomenon, whereas those from sea-fish and sea-water fail to show such an activity. In this paper, a hemolytic activity of the supernatant of the broth culture was investigated and, at the same time, its relationship with Kanagawaphenomenon was studied.
    Strongly Kanagawa-phenomenon positive strains were inoculated into brain heart infusion broth and incubated in 37°C water bath with rocking culture method.
    Supernatant fluid of the broth culture showed strong hemolytic activity against human red cells and lethal toxicity to mice, but, no effect to horse red cells. These activities were resistant to heating at 100°C for 30 min. but inactivated by tripsin. The production of the cytolytic factor was observed in the period of logarithmic multiplication of the vibrio.
    On the other hand, another hemolytic factor which was revealed thermolabile and resistant to tripsin was recognized in the lysate of Vibrio cells not only from Kanagawa-phenomenon positive but also negative cultures. This hemolytic factor lysed both human and horse red cells but demonstrated no toxicity to mice.
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  • Studies on Hemolytic Factors of Vibrio parahaemolyticus
    II. The Extraction of Hemolysin and its Properties
    Yasushi OBARA
    1971 Volume 45 Issue 9 Pages 392-398
    Published: September 20, 1971
    Released on J-STAGE: September 07, 2011
    DOIhttps://doi.org/10.11150/kansenshogakuzasshi1970.45.392
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    The extraction of hemolysin from the supernatant fluid of the brain heart infusion broth culture of a strongly Kanagawa-phenomenon positive strain of Vibrio parahaemolyticus was carried out by means of precipitation with ammonium sulfate, acid precipitation with acetic acid, and Sephadex G-200 gel filtration.
    The hemolysin fraction possessed an activity of 7, 692 MHD/mg (dry weight) against human red cells and LD50 of 4.9 mcg for mice. (MHD=minimum hemolytic dose)
    The nature of the fraction was chiefly a protein and no reaction was recognized as polysaccharides, lipids and organic phosphorus. Its lytic activity was enhanced in the presence of Ca++, inactivated by tripsin, and tolerated the beating at 100°C for 30 min. at pH 6.0.
    Red cells of dog, mouse, rabbit, monkey, guinea pig and chicken were also suspectible to the hemolysin but those of horse were not. The hemolytic reaction was weak in sheep red cells. The fraction lysed Hela cells and its intracutaneous injection caused blisters in mice.
    From these biological properties, the hemolysin was regarded as one of morbidity Factors of Vibrio parahaemolyticus.
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  • Immunofluorescent Antibody-titers in Mycoplasma pneumoniae Infections
    Masao UMETSU, Tooru Nakao
    1971 Volume 45 Issue 9 Pages 399-405
    Published: September 20, 1971
    Released on J-STAGE: September 07, 2011
    DOIhttps://doi.org/10.11150/kansenshogakuzasshi1970.45.399
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    Immunofluorescent (IMF) technique has been proved the most useful in the detection of antibodies against Mycoplasma pneumoniae (M. pneumoniae) infections. However, difficulty of preparing antigens has interfered with extensive employment of this technique.
    The purpose of this study was to detect the antibodies to M. pneumoniae by indirect IMF test, utilizing coverslip grown colonies which were proved recently a simple and practical antigen.
    In this test, the specific antigen-antibody reactions appeared to be detectable rapidly, and, thereby large clumbs of colonies, which fluoresced more birghtly than small, ones, had a tendency to show higher endpoints of serum dilutions.
    A combination of IMF and complement fixing tests increased the reliability of the diagnosis. However, a strict correlation was not always seen between the titers measured by these two tests.
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  • [title in Japanese]
    [in Japanese]
    1971 Volume 45 Issue 9 Pages 406-419
    Published: September 20, 1971
    Released on J-STAGE: September 07, 2011
    DOIhttps://doi.org/10.11150/kansenshogakuzasshi1970.45.406
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  • [title in Japanese]
    [in Japanese]
    1971 Volume 45 Issue 9 Pages 420-422
    Published: September 20, 1971
    Released on J-STAGE: September 07, 2011
    DOIhttps://doi.org/10.11150/kansenshogakuzasshi1970.45.420
    JOURNAL FREE ACCESS
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    [in Japanese]
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  • [title in Japanese]
    1971 Volume 45 Issue 9 Pages 423-425
    Published: September 20, 1971
    Released on J-STAGE: September 07, 2011
    DOIhttps://doi.org/10.11150/kansenshogakuzasshi1970.45.423
    JOURNAL FREE ACCESS
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