To contribute to the clarification of the developmental process of human intestinal infections, experimental studies were done producing salmonellosis on monkeys (cynomolgus). And we are reporting a series of experiments and in this paper isolations of immune globulins from the serum and intestinal tissues of the monkeys were performed as the preliminary experiments.
Results were summarized as follows:
I) Monkey IgM, IgG, and IgA were cross-reacted with human IgM, IgG, and IgA, respectively, and the common antigens were recognized, respectively.
II) Separation of IgM: Precipitates of the whole serum by 33% Am Sul were fractioned by gelfiltration on the column of Sephadex G-200, and effluents were separated into two peaks (18s and 7s), and IgM was obtained by zone electrophoresis in 18s fraction.
III) Separation of IgG: Above obtained 7s peak was separated by DEAE chromatography using 0.01 M PH 7.4 PBS.
IV) Separation of IgA: From the extracts of monkey intestine tissue, y fraction was obtained by zone electrophoresis. This fraction was chromatographed on DEAE-cellulose, and the effluent obtained through PH 6.4, 0.10 M was further refined through Sephadex G-200 gel.
V) H.L. chain separation from IgG: Refined IgG was reduced by 2-ME and alkylated by iodoacetamide, and then geI-filtrated through Sephadex G-200 in 1 N H. Ac solution at the room temperature. Heavy and light chain were thus separated. Proportion of H and L chain was 74: 26.

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Fourteen cynomolgus monekys, weighed 2-4 Kg, were orally given salmonella typhimurium or salmonella enteritidis (3-5×10^7-8/ml cell) after fasting for two days. CaCO³ of 500mg was also given immediately before the bacillary administration. Clinical course, histopathological findings of the intestine, bacillary invasion and its habitation in the intestine and other organs, and mucous antibodies in the intestinal mucous tissue, spleen and lymphglands were investigated using immunofluorescence method among others. Although two salmonella species were used in this experiment, any mentionable difference couldn't be found in the results between the two and, thus, a general summarization was drawn as follows:
1) The onset of the illness was 48-72 hours after the bacillary administration in general.
2) Twenty four hours after the onset of the illness, salmonella bacilli were seen invading into the small intestine, the cecum, and the rectum.
3) By 48 hours after the onset of the illness, the bacillary invasion into the small intestine was seen quantitatively decreasing and around 72 hours after the onset of the illness the bacillary growth in the cecum and the rectum became the greatest. If the animals survived the crucial 96 hours after the onset of the illness, they improved gradually, and after one week bacillary invasion couldn't be observed any more. However, in only one case which got into carrier state, a large quantity of the bacilli was found invading and habitating in the cecum, the rectum and lymphglands even after 2 weeks after the onset of the illness.
4) During acute stage, bacillary invasion could be seen in the lymphglands, the spleen and the gall bladder.
5) In the intstinal mucous membrane, IgA found in lamina propria became secretory IgA in coincidence with bacillary invasion. IgM and IgG are principally found in Lamina propria, but it appeared in this experiment that IgM is sometimes secreted. Impression is that IgA is most closely related with the phase of the bacillary invasion.
6) In the lymphnodes and spleen, IgM appeared first and most prosperous coincided with acute stage of bacillary invasion. IgG appeared a little while later. One carrier state monkey investigated had shown no IgM producing cell in the lymphnodes and spleen.

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Hyuganetsu disease was caused by ingestion of raw gray mullet, indicating an endoparasite-born rickettsios is.
Attempts were made to isolate causative rickettsia from the parasites of the fish. Metacercaria of Stellantchasmus falcatus, homogenized or untreated, were inoculated into mice via peritoneal or oral routes. The mice were autopsied after 14 days and 30 days, respectively, and thereafter blind passages were done every 14 days with spleen homogenate. Four strains of rickettsia-like organisms were isolated by the former procedure and 6 by the latter.
These rickettsia-like isolates were studied for its characteristics comparing with Rickettsia sennetsu. They shared common morphological, biological and antigenic properties on the one hand such as:
1. They were visualized by Macchiavello staining of lymph node cells of infected mice; coccoid and pleomorphic.
2. They were unfiltrable through Chamberland L3 filters.
3. They were sensitive to heat treatments (50°C, 10 minutes), formalin (0.1%, 3 hours), phenol (0.5%, 3 hours) and ether (10%, 30 minutes).
4. They were sensitive to antibiotics.
5. The isolates were positive for direct immunofluorescence test with labelled anti-Rickettsia sennetsu antibody or for indirect method with convalescent patient serum of “Hyuganetsu” disease and labelled anti-human-γ-globulin antibody, and Rickettsia sennetsu was also positive for indirect method using convalescent mice serums infected with the isolates and labelled anti-mouse-γ-globulin antibody.
However, the pathogenicity and some other characteristics distinguished them from Rickettsia sennetsu on the other:
1. They did not kill mice while Rickettsia sennetsu did in 14 days.
2. Autopsy of the infected mice revealed no viscous exudate in the peritoneal cavity.
3. Mice immunized with the isolates acquired only a little cross immunity against Rickettsia sennetsu infection.
The possibility that the metacercaria of Stellantchasmus falcatus may carry Rickettsia sennetsu as well as the rickettsia-like organism is not inconceivable in the light of the fact that Nanophyetus salmincola, the vecter of salmon disease complex in dogs, can be concurrently infected with Neorickettsia helminthoeca and Elokomin fluke fever agent.

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[in Japanese]

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[in Japanese]

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[in Japanese]

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