Kansenshogaku Zasshi

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The present study describes the isolation of Pseudomonas aeruginosa from river waters in Toyama Prefecture and the comparison of serotype distribution between isolates from river waters and from various clinical specimens.
Both 37°C and 42°C should be used in enrichiment cultivation with liquid NAC (nalidixic Acid Cetrimide) medium in order to obtain maximum detection rate of Pseudomonas aeruginosa from river waters. The use of the two different temperaturs often resulted in isolating different serotype strains in the same water sample. The phenomenon may be caused by the coexisting organisms of other species in the river water and the delicacy of optimum temperature for the growth of pseudomonas aeruginosa.
The overall detection rates of pseudomonas aeruginosa from river waters in Toyama prefecture were 65.7%, among which 3 rivers in Toyama city showed the highest rate of 86.7%, rivers in the western half of the Toyama prefecture, 61.7%, and rivers in the eastern half, the lowest rate of 38.4%.
Mean viable cell counts and E. coli, group MPN were significantly higher in river waters where pseudomonas aeruginosa were detected in comparison to those in river waters where this organisms were not detected.
Dominant serotypes of isolates from river waters were, from the top, type 8 (39.0%), 10 (11.0%), 5 (9.0%), 6 (5.7%), 7 and 9 (5.2% each), whereas those from clinical specimens were type 8 (28.4%), 5 (14.4%), 10 (9.4%), 7 (8.2%), and 13 (7.9%). The both isolation patterns of serotypes were significantly resembling. In patient-enviroment-cicle, type 8, 5 and 10 are particularly regarded as important species.

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1. The first report of subphrenic abscess due to Peptostreptococcus morbillorum in Japan was presented. The patient was eighty-seven years old female and died of myocardial infarction directly. Clinical and laboratory findings were described in detail in this paper.
2. The diagnosis of subphrenic abscess was not made clinically, but at autopsy Peptostreptococcus was isolated purelly in anaerobic atmosphere from the pus of the infected foci. In pathologic examination, the organisms were seen stained gram positive in the histiocytes or polynuclear leucocytes infiltrated in the abscess tissue.
3. This organisms is highly susceptible to many antibiotics except for aminoglycosides and colistin.

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