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Volume 48, Issue 6
Displaying 1-5 of 5 articles from this issue
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3. Distribution in foodstuffs and serotypesAkira SUGITANI1974 Volume 48 Issue 6 Pages 201-210
Published: June 20, 1974
Released on J-STAGE: September 07, 2011
JOURNAL FREE ACCESSFood samples were examined for the presence of C. perfringens and the ser ological investigations of the isolates were performed by agglutination test.
1. The distribution of C. perfringens was 2% in the heated and cooked food samples. Positive rate was 1 (9%) of 11 samples of Kamaboko (boiled fish paste). In Kamaboko, C. perfringens increased from 200/g to 20× 104/g in viable count while it was kept at 37°C for 48 hr.
2. The distribution of C. perfringens was 33% in raw food, i. g. 100% in 10 samples of meat, 30% in 10 Sashimi (slices of raw fish) and 89% in 9 oysters. In the positive samples C. perfringens did not multiply under the same conditions as above.
3. The heat-resistant strains of C. perfringens were found only in 4 of 9 samples of oysters.
4. The viable cell counts of C. perfringens in all samples were less than 200/g.
5. Meat, Kamaboko and Sashimi had, respectively, one serotype of C. perfringens per one sample, but 3 samples of the oysters which were contaminated by the heat-resistant strains had 2, 2 and 5 serotypes, respectively.
6. Complex antigenic relations were found among the heat-resistant strains isolated from the oysters, but they had no similar antigenic structures. The heat-labile strains of C. perfringens from other food samples had no serological relations to each other and showed high degree of strain-specificity.
7. One of the 8 serotypes in the heat-labile strains of C. perfringens had serologically a close re-lationship with a heat-resistant strain which was isolated from a case of food-poisoning. However, there was no completely the same antigenic structure between these two strains.View full abstractDownload PDF (1218K) -
Kazuhiro KOBAYASHI, Toshiyuki KITAURA1974 Volume 48 Issue 6 Pages 211-219
Published: June 20, 1974
Released on J-STAGE: September 07, 2011
JOURNAL FREE ACCESSAn improved procedure, modified enrichment serology procedure (ES), for rapid detection of Salmonella is presented. Employing this procedure, Salmonellae can be detected from meat and feces, within 46 and 28 hours, respectively, while 4 to 5 days are necessary for the isolation of the bacteria employing usual culture method (BC).
Salmonellae were detected from 19 specimens out of 84 (prepared from 16 different meat stocks) by employing BC, ES and ES culture method (ESC). Positive detection rates of Salmonella by these procedure were 94.7%(18/19), 89.5%(17/19) and 94.7%(18/19), respectively, for BC, ES and ESC.
The total number of strains detected from these 19 specimens was 29 (twenty-four strains were isolated by BC and ESC). The number of bacterial strains detected was 24 from 17 specimens for ES, 20 from 18 specimens for BC and 23 from 18 specimens for ESC. By employing ES, it is possible to guess the serotype of detected Salmonella strains without performing further isolation and identification procedures, if the detected bacteria belong to frequently detectable serotypes.
Since ES can be performed more quickly and is simpler than BC which is laborious and expensive, this seems to be a useful procedure for the detection or screening of Salmonellae in meat, sewage and feces.View full abstractDownload PDF (1385K) -
Yutaka KANAZAWA, Kengo IKEMURA, Itaru SASAGAWA, Naoya SHIGENO1974 Volume 48 Issue 6 Pages 220-228
Published: June 20, 1974
Released on J-STAGE: September 07, 2011
JOURNAL FREE ACCESSA 26-year-old housewife was admitted because of right lower quadrant pain of 4 days duration, vomiting, dizziness and fever. At the time of admission, body temperature was 38.2°C and leucocyte count was 8200 per cu mm. Percussion tenderness on abdomen was maximum at McBurney's point but muscular guarding and Blumberg's sign were equivocal.
She was treated symptomatically without laparatomy. Abdominal pain decreased in severity with subsiding of fever within 10 days after the onset of illness. Slight ileocecalgia, however, had persisted for about one and half months.
Alimentary X-ray examination on the 17th and 34th days of illness revealed hyperplasia of mucosal folds and stenosis of the terminal ileum with regional tenderness. X-ray examination, however, on the 72nd day, when she had become free from complaints, disclosed resolution of the pathological findings before observed. Thus diagnosis of terminal ileitis was decided.
A strain of Yersinia was isolated from feces of the 9th day of illness. Its biochemical properties were identical to Y. pseudotuberculosis. Cross-absorption test for O-antigen showed that the strain was antigenically similar to the reference strain, Y. pseudotuberculosis type III (No.83).
Agglutinin titer of convalescent serum to O-antigen of patient's own strain was 1: 160 on the 34th day.
The organism, when isolated, was non-motile but became motile after serial subcultures at 25°C using Cragie's tubes. The motile strain showed several peritrichous flagella under electron microscope.
Single-disc sensitivity test revealed the organism to be sensitive to benzyl-penicillin, cephaloridine, gentamicin, nalidixic acid and sulfisomezole and resistant to streptomycin, erythromycin and spiramycin.View full abstractDownload PDF (7546K) -
[in Japanese]1974 Volume 48 Issue 6 Pages 230-232
Published: June 20, 1974
Released on J-STAGE: September 07, 2011
JOURNAL FREE ACCESS -
1974 Volume 48 Issue 6 Pages 233-235
Published: June 20, 1974
Released on J-STAGE: September 07, 2011
JOURNAL FREE ACCESSDownload PDF (420K)
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