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49 巻, 1 号
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特に成立機序を中心として飯村 達1975 年 49 巻 1 号 p. 1-4
発行日: 1975/01/20
公開日: 2011/09/07
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第1報緑膿菌の無機塩水における増殖萩原 義郷, 伊藤 亨, 脇 千賀子, 中村 昌弘1975 年 49 巻 1 号 p. 5-11
発行日: 1975/01/20
公開日: 2011/09/07
ジャーナル フリーIn the previous paper, it was demonstrated that Pseudomonas aeruginosa propagated in city water, but did not grow when the city water was deionized by an ion-exchange resin. In order to know what kind of component in city water is essential for the growth of the bacilli, some fundamental experiments were performed.
The present paper primarily describes the studies on the growth of Pseudomonas aeruginosa in simple solutions artificially composed of inorganic salts only.
The results obtained are as follows:
1) It was noted that Pseudomonas aeruginosa was able to grow in inorganic salt solutions. It was clearly determined that, within the limited studies performed here, the bacilli easily multiplied in the solution containing only CaCl2 and Na2HPO4 at the final concentration of 0.01%.
2) It was further confirmed that Pseudomonas aeruginosa definitely multiplied in the mixture solution of CaCl2 and Na2HPO4, even when the inoculum size was extraordinarly small.
3) Furthermore, the serial subcultivation of the bacilli in this mixture solution was possible. Therefore, it could be mentioned that the phenomenon that the bacilli multiplied in this solution should not be false but genuine growth.
The data, repo' ted here, concerning the discovery of the minimum essential growth factor for Pseudomonas aeruginosashould contribute to the studies on drug-fastness of this organism and reconstruction of bacterial cells.抄録全体を表示PDF形式でダウンロード (861K) -
続報種々の検体におけるEnrichment serologyの応用小林 一寛, 宮田 義人, 北浦 敏行, 高浜 佳代子, 西田 京子, 宮地 恵子1975 年 49 巻 1 号 p. 12-17
発行日: 1975/01/20
公開日: 2011/09/07
ジャーナル フリーVarious samples (human and rat feces, chicken and beef meat) were examined for the presence of Salmonellae employing conventional plating method (BC), ES procedure described in the previous paper and ES cultural method (ESC). The purpose of this paper is to evaluate the ES and ESC procedure for the rapid detection of Salmonellae and to compare the results of these procedures with that of BC procedure.
The results were summarized as follows:
1) Out of 161 samples, 37 strains of Salmonellae from 35 samples were isolated employing 2 cultural methods (BC and ESC). Positive detection rates of Salmonella were 88.6%(31/35), 94.3%(33/35) and 82.9%(29/35), respectively for BC, ESC and ES, However, 2 Salmonella serotypes identified from 2 samples by employing BC and ESC could not be detected by ES, because the antisera used in ES alone could not identify the 2 serotypes.
2) Twenty-six samples were positive, and 123 samples were negative for Salmonella by all of BC, ESC and ES. The results obtained by 3 methods were coincidental by 92.5%.
3) Two Salmonella positive samples were enriched with 24 hours selective enrichment ES pro-cedure, although other 2 positive samples with 5 hours ES procedure turned negative for Salmonella by 24 hours ES procedure. These trends were remarkable in the case of the detection of somatic antigen employing ES procedure. For this reason, 24 hours ES procedure must be performed after the 5 hours ES procedure was finished.
4) ES procedure for the detection of somatic and flagellar antigens appears to be a simpler, specific, rapid and satisfactory method for the detection of Salmonellae.抄録全体を表示PDF形式でダウンロード (712K) -
小野 義三, 高橋 千恵, 三好 薫, 出口 祐男, 佐々木 盛生, 野田 和秀1975 年 49 巻 1 号 p. 18-25
発行日: 1975/01/20
公開日: 2011/09/07
ジャーナル フリーThe complement-fixating (CF) antigen was prepared in human embryo lung cells infected with varicella-zoster virus (VZV). CF antibody titers in 22 patients with herpes zoster were examined, followed up and the relation between VZV infection and CF antibody was studied. Four hundred and fifty six normal sera were also examined for the control with the consideration of the age.
Out of 22, 19 were observed to have an increase in CF antibody titer during the period between the acute and the convalescent phases. The titer seemed to be proportional to clinical severity of the infection in the patients. After three months of infection, the CF antibody, decreased rapidly.
The percentage of positive CF titer (≥1: 8) in normal persons was 37.1. This was unexpectedly low compared with that appeared in reports abroad. Positive rates of CF antibody on age basis were highest in the 20s and 50s, and lowest in the early teens and the 30s. Among healthy persons, 25 cases (5.48%) and 11 cases (2.41%) were found to show a high CF antibody titer of more than X32, and X64, respectively. Presumably, this result can be interpreted as reactivation or reinfection of VZV which would, in most cases, occur in non-manifested form when the antibody titer dropped low enough to permit the virus to get active.抄録全体を表示PDF形式でダウンロード (3491K) -
1975 年 49 巻 1 号 p. 26-28
発行日: 1975/01/20
公開日: 2011/09/07
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1975 年 49 巻 1 号 p. 29-31
発行日: 1975/01/20
公開日: 2011/09/07
ジャーナル フリーPDF形式でダウンロード (422K)
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