Kansenshogaku Zasshi

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A following equation, which was concerned in mathematical study of the voiding defense mechanism of the urinary bladder, was proposed in the previous report.
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To solve the equation, two parameters, values of generation time of organism and normal residual urine, were experimentally found out.
Approximately 400ml of tab water to which a couple to drops of anoinic surface-active agent were added was poured into each of 52 artifficial models of the bladder made from either latex or nylon. After the model was emptied either the way the water was let out with the model turned upside down, the way shaked out, or the way squeezed out, amount of water which was left behind on the surface of the model was measured. Value of 0.3ml was obtained as normal residual urine.
Ten ml of nutrient broth was inoculated with 10² cells of organisms and icubated at 37°C. Their generation time was calculated from numbers of the organisms counted 2 and 4 hours later by poured plate method. E. coli NIHJ JC-2 was added to each group of several strains of organisms as indicater representing accuracy of the measurement. Its generation time was 24′30′′±59′′ in average and its 95% confidence interval estimate 24′30′′±37′′. If the generation time of E coli NIHJ JC-2 which the group included did not fall within 24′30′′±37′′, the experiment with the group was made again.
Generation time of 19 strains of E. coli, 9 strains of Klebsiella, 17 strains of Serratia, 16 strains of Pseudomonas aeruginosa and 8 strains of Rettgellera which were isolated from urine of patients with urinary tract infection during the 5-month period from August until December 1974 at the urological ward of Yamaguchi University Hospital was 24.1′±3.5′, 23.0′±2.5′, 28.8′±4.0′, 27.2′ 4.8′ and 37.9′±4.4′, respectively.

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A 26-year-old male was admitted to Itabashi Nihon Univ. Hosp., with the diagnosis of acute myelogenous leukemia. Soon after the admission he was started to have antileukemic chemotherapy with Daunomycin, Cy. arabinoside, 6-MP and Predonine.
On the twentieth hospital day, he was noticed to have high fever, 39°C, and continued to have high fever for nine days. On the first, third and fifth day of fever, venous blood were drawn and cultivated Bacillus cereus. Bacillus cereus was also cultivated from pharyngeal swab.
He became afebrile by chemotherapy of GM, CER and LCM, but died on the forty-eight hospital day with complication of hemorrhagic diathesis.
The isolated Bacillus was compared with Bacillus subtilis ATCC 6633, biologically.

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