A large role in the defence mechanism against bacterial infection is attributed to the phagocytic reaction of white blood cells.
We reported that anti-OEP-IgG was effective against the experimental Pseudomonas aerugionosa infection in mice, even when in the immunosuppressive stage. At the same time, large monocytes in spleen were observed ingesting many bacteria.
Unfortunately, however, almost all of the antibody produced againt Gram-negative bacteria is in the IgM fraction of blood serum, so, it is very difficult to use for therapy in humans.
Recently, we observed occasionally that anti-OEP-IgG was contained in the commercial gammaglobulin products, although its titer was not so high.
The following experiments wer attemped to reveal (1) whether it was clinically feasible to use this anti-OEP-IgG in such a low titer, (2) the relationship between the marcophage, the anti-OEP-globulins and the bacteria in the activity observed. (3) whether the macrophage's phagocytic reaction to P. aeruginosa was affected more strongly by IgG or by IgM. (4) the difference of the macrophage's phagocytic reaction against P. aeruginosa versus the OEP-coated Latex Particles; this was donein order to reveal the mechanisms of P. aeruginosa's anti-phagocytic nature if there was a synergistic effect when using egntamicin together with the immunotherapy.
The antibody significantly enhanced the macrophage's phagocytosis against P. aeruginosa, also the IgG fraction had stron ger activitythan the IgM fraction. By addition of gentamicin, the phagocytic reactions were significantly strengthend statistically. There was no signifficant difference in the phagocytic activity against either P. aeruginosa or the Latex particle. The adhesive reaction between the antibodies and the bacteria was remarkable but not between the antibody and the macrophage. Also, the adhesive reaction between the atibody and the bacteria was enhanced by gentamicin. The activity of the IgG was stronger thanthat of the IgM, too. All the experiments were performed within the clinically accepted doses for anti-OEP-IgG and gentamicin.

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In order to determine the therapeutic effectiveness of γ-globulin products for intravenous use against Paeruginosa infection, we tried the following experiment. OEP-, elastase-, protease-HA titer were measured respectively for these intravenouse γ-globulin products (2 Companies, 3 Products, 53 Lots). OEP-HA titer is higher in B Co.'s products than A Co.'s.
In proportion to OEP-HA titer, the enhancement of the macrophage's opsonic activity against P. aeruginosa was found to be propotional to OEP-HA titer by radioimmunoassay with labaled the globulins.
The therapeutic effectiveness of each γ-globulin product against mice experimentally infected with P. aeruginosa (NC-5 strain) was also propotionate to its OEP-HA titer. The combined therapy of γ-globulin products, Gentamicin and Apacillin enhanced the curative effectiveness against P. aeruginosa infection in mice.
Non-specific effects against P. aeruginosa were not observed in these γ-globulin products. Protective activity against P. aeruginosa infection was directly correlated to the OEP-HA titer.

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A 80 year-old man was diagnosed as having gastric cancer by an upper G. I. series. During the course of the therapy with blood transfusion and predonisolone, acute pneumonia developed. A gram stain on direct smears from the sputum revealed numerous gram -negative diplococci in the polymorphonuclear cells and culture of the specimen yielded abundant colonies of Brahna-mella catarrhalis.
Carbenicillin and cephalothin were effective against that pneumonia, but soon after slight improvement, E. coli pneumonia developed and the patient died
Neeled biopsy specimen of the lung revealed the histological picutre of bonchopneumonia

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