Serratia marcescens strains isolated from various clinical materials and several places in Nagasaki University hospital during the period from April, 1970 to March, 1976 were classified by bacteriocin (marcescin) production typing by means of the agar cross streaking method (no induction and 8 indicators used). The epidemiological analyses of these results were carried out.
1) Five hundred and fifty four isolates (333 from urine, 144 from sputum, 51 from pus & exsudate, 8 from bile and 18 from others) in the above period were classified into 26 types by bacteriocin typing and those strains had a relatively charcteristic distribution in bacteriocin types according to each clinical material.
2) The epidemiological investigations were performed using the isolates obtained from the patients and places in the hospital during the period of 15 months from January, 1975 to March, 1976. Each ward had a distinctive distribution in bacteriocin types of the isolates.
The bacterial flora in 100 places of each ward was examined; 5 strains of Serratia marcescens from the gynecologycal ward (4 from a washroom, 1 from a treatment room) and 5 from the urological ward (2 from a washroom, 2 from a floor, 1 from a bed) were obtained. The bacteriocin types of themwere included in main types of the isolates from the patients admitted to both wards except 1 strain from the treatment room of the gynecological ward.
3) Six patients were studied for comparison of the bacteriocin typing of Serratia marcescens isolated from several materials taken from the same patints. Three out of these patients demonstrated the identical bacteriocin type even in different materials individually, but the others showed the different bacteriocin type of Serratia marcescens.
Furthermore, the state of keeping, isolation and communicalbe form of Serratia marcescens among the hospitalized patients were discussed by bacteriocin typing.
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