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53 巻, 12 号
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池本 秀雄, 森 健1979 年 53 巻 12 号 p. 685-686
発行日: 1979/12/20
公開日: 2011/09/07
ジャーナル フリーPDF形式でダウンロード (334K) -
榊原 久雄, 田辺 巌, 江本 雅三1979 年 53 巻 12 号 p. 687-693
発行日: 1979/12/20
公開日: 2011/09/07
ジャーナル フリーA strain of V. cholerae isolated from a cholera patient found in Kobe was proved to have the following charactristics:
1) The strain grew very slowly or was distinctly undergrown on TCBS agar which can well support the growth of V. cholerae organisms generally. It took 36-48 hrs before the formation of visible colonies (1-2 mm in diameter). At earlier stages of growth (about 18 hrs after the beginning of incubation at 37°C), the colonies had greenish tone, instead of yellowish tone as generally shown by the V. cholerae organisms. This was due to the lack of ability of decomposing sucroce. However, after 24 hrs of incubation, the colonies became yellowish. The same statement was made as to the results using peptone water. In general properties, this strain resembled those successively cultivated in peptone water.
2) Since there are V. cholerae strains which can grow very slowly on TCBS agar, it is absolutely necessary to use additionally other kinds of selective media, such as alkaline agar or Endo's agar, for the purpose of primary isolation of V. cholerae from natural sources.
3) Other characteristics such colony morphology, biochemical activities, sugar decomposition capacities and agglutinability against antiserum, etc. were essentially the same as those of typical V. cholerae. However, no Kappa type phage was isolated. Based on these data, we regarded this strain as classical El Tor vibrio of the continental type.抄録全体を表示PDF形式でダウンロード (3512K) -
寺島 英一, 土屋 俊夫, 奥山 清子1979 年 53 巻 12 号 p. 694-697
発行日: 1979/12/20
公開日: 2011/09/07
ジャーナル フリーRecently we found that distilled water in sickroom of pulmonary tuberculosis was heavily contaminated with acid-fast bacteria and much acid-fast bacteria were isolated from gargled water of many patients, So we investigated the distribution of acid-fast bacteria in hospital, and found that much acidfast bacteria exist in flower-vase-water, distilled water and water in incubater. These acid-fast bacteria isolated from water were identified all as M. aquae. M. aquae truly isolated from respiratory tract were investigated, and its pathogenicity also was discussed.抄録全体を表示PDF形式でダウンロード (541K) -
中村 和幸, 西沢 修一1979 年 53 巻 12 号 p. 698-703
発行日: 1979/12/20
公開日: 2011/09/07
ジャーナル フリーWith a view to make early laboratory diagnosis of the influenza, HA production test of influenza virus and isolation of virus from patient's samples were carried out by use of the MDCK cell suspension, with the following results obtained.
The best HA production was obtained for both influenza virus A and B in 2×105 cells/ml of cell suspension. Fluid maintenance medium was Eagle's MEM cotaining twice as much glucose, four times as much vitamins, 20μg/ml of trypsin and 10% tryptose phosphate broth. Thus, using this method, virus isolation was made from the samples of some patients during influenza epidemics, and as a result, 16 strains of virus were isolated from 70 samples of first passage with results favorably comparable with such a method as using MDCK cell monolayers or fertile hen's eggs.抄録全体を表示PDF形式でダウンロード (883K) -
永井 隆雄1979 年 53 巻 12 号 p. 704-712
発行日: 1979/12/20
公開日: 2011/09/07
ジャーナル フリーUrinary tract infections (UTI) have been investigated in 1082 patients (422 males and 660 females) of all ages with bacterial counts above 100, 000 organisms per ml in urine. The patients were seen at University of Kyoto Hospital between March, 1975 and February, 1978.
The organisms isolated from urine containing more than 100, 000 bacteria per ml were surveyed after quantitative bacterial culture from mid-stream urine. The most frequently isolated bacteria in the patients overall were Escherischia coli (28%), Proteus spp. (15%), Pseudomonas aeruginosa (13%), and Klebsiella spp (12%). In male patients, Proteus spp. (20%) were most frequent, followed by E. coli (17%), Ps. aeruginosa (15%), Klebsiella spp. (13%), and enterococcus (13%).
In female patients, E. coli (40%) was most frequent, followed by Proteus spp. (12%), Klebsiella spp. (11%), enterococcus (11%), and Ps. aeruginosa (10%). E. coli was the predominant isolate in females, both adults and children, being present in approximately 40% to 60% of positive cultures.
In male patients, no isolate was predominant; the percentage of E. coli isolates was lower, and those of Proteus spp., Klebsiella spp., Ps. aeruginosa, and enterococcus werehigher than in females.
In UTI in patient without underlying diseases, E. coli was the predominant isolate, being present in 40% of the positive cultures, while in patients with malignancy, the most frequent isolate was Ps. aeruginosa (20%), followed by E. coli (18%), enterococcus (18%) and Proteus spp (13%).
And in UTI in patients with nephrolithiasis, Proteus spp. (25%) was the most frequent isolate, followed by E. coli (20%), Staphylococcus spp. (10%), Klebsiella spp. (10%), Enterobacter spp. (10%), and Ps. aeruginosa (9%).抄録全体を表示PDF形式でダウンロード (999K) -
福田 武夫, 山本 正悟1979 年 53 巻 12 号 p. 713-716
発行日: 1979/12/20
公開日: 2011/09/07
ジャーナル フリーAntigenic relations of SF strain (i. e. rickettsia-like isolate from
Stellantchasmus falcatus) to Rickettsia sennetsu and Rickettsia tsutsugamushi were investigated by direct fluorescent antibody technique and fin estructures of the organism grown ill RK-13 cell were observed by electron microscopy.
1) Results of direct fluorescent antibody technique
In direct immunofluorescence, undiluted labeled antibodies to each of the three agents, which were refined from sera of guinea-pigs injected intracerebrally with those agents respectively and were labeled with fluorescein isothiocyanate, stained only homologous agent.
From this result it should be concluded tha t these agent had reciprocally different specific antigens and no cross-reacting ones.
2) Results of electron microscopic observations
SF strain was found in cytoplasm cf host cell and seen as short-rod-shaped (about 0.3 to 0.7 micron wide and up to 1.0 micron long) and pleomorphic organism. The organism was enclosed by trilaminar cell wall and trilaminar plasma membrane, and the internal structure was made upof electron dence granular ribosomes and irregular pale patches containing DNA strands. The organisms, singly or in aggregate, were contained in vacuoles of host cell cytoplasm.
The presence in vacuole of host cell and the fine structure of SF strain resemble those of Coxiella burnetii, Neorickettsia group (Neorickettsia helminthoeca and Elokomon fluke fever agent) and R. sennetsu, but SF strain is different in kind of vector from Coxiella burnetii and in antigenic structure from R. sennetsu. Although the vector pf SF strain differs from that of Neorickettsia group and antigenic relations between them are not investigated, SF strain may be a unique member of Neorickettsia, because i+ is transmitted by endo-parasite and pathogenic for mouse.抄録全体を表示PDF形式でダウンロード (4039K)
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