Kansenshogaku Zasshi
Online ISSN : 1884-569X
Print ISSN : 0387-5911
ISSN-L : 0387-5911
Volume 65 , Issue 5
Showing 1-17 articles out of 17 articles from the selected issue
  • Hisashi NAKAHATA, Yuichi HIRAI, Moriyasu TSUJINO, Rumiko SAWADA, Yoshi ...
    1991 Volume 65 Issue 5 Pages 521-526
    Published: May 20, 1991
    Released: September 07, 2011
    JOURNALS FREE ACCESS
    The superoxide anion (O2-) production in polymorphonuclear leukocytes stimulated by phorbol myristate acetate in IDDM and non-insulin dependent diabetes mellitus (NIDDM) was determined by the method of Johnston et al, compared with that of each age matched controls. And the correlation between O2- production and hemoglobin (Hb) Al and Alc value was investigated.
    The O2- production in IDDM was 24.4±7.4 (mean±SD, n mol per 4×105 cells) at 10 min. and 51.4±8.7 at 30 min., in NIDDM each 31.6±9.3, 60.2±14.4, and in controls each 40.5±4.2, 72.4±3.1. O2- production in IDDM was significantly lower than that in NIDDM (p<0.001 at 10 min. and p<0.0130min.) and controls (p<0.001 at 10 and 30 min.).O2- production at 10 and 30 min. possessed a negative correlation with Hbal and Alc value (HbAl: p<0.01 at 10 min. p<0.05 at 30 min., HbAlc: p<0.01 at 10 and 30 min.).
    These findings suggest that impaired O2- production might be one of the factors accounting for depressed bactericidal activity of polymorphonuclear leukocytes in IDDM, and that a protracted hyperglycemia might shed some effect on O2- production.
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  • Kazuyuki IGUCHI, Masato KAWABATA, Yasutomo ARASHIMA, Nobuyuki KUBO, Mi ...
    1991 Volume 65 Issue 5 Pages 527-530
    Published: May 20, 1991
    Released: September 07, 2011
    JOURNALS FREE ACCESS
    The serological survey, analyzing sera which were obtained from Lyme disease suspected subjects was performed using immunoperoxidase (IP) test between July, 1987 and February, 1989. Fifteen seropositive subjects to Borrelia burgdorferi have been found in this survey, and these cases were considered to be true Lyme disease or highly suspected for this illness. Most of these seropositive cases geographically occurred in northern part of Japan, and both sexes and all age subjects were affected. In clinical manifestations, 5 cases developed a typical skin lesion of erythema chronicum migrans (ECM) followed by tick-bite. Neurologic manifestations were found in 6 cases, including one patient who also had ECM after a known tick bite. Of these neurologic manifestations, 4 cases had lymphocytic meningitis, 3 unilateral or bilateral facial palsy, and one encephalomyelitis. When 8 of these 15 sera were analyzed for antibodies to Lyme disease with ELISA, a poor agreement of serodiagnosis between IP test and ELISA has been found in the course of this study.
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  • Yotaku GYOBU, Hirohide KODAMA, Shigeaki SATO
    1991 Volume 65 Issue 5 Pages 531-536
    Published: May 20, 1991
    Released: September 07, 2011
    JOURNALS FREE ACCESS
    Enteropathogenicity and plasmid DNA of clinical and environmental isolates of non-O1 V. cholerae were examined. Results were as follows: 1). The frequencies of enteropathogenic strains judged by the results from both ligated rabbit ileal loop (RIL) and suckling mouse tests were 36/38 (95%) for isolates from oversea travellers, 15/15 (100%) for isolates from food poisoning, 33/44 (75%) for isolates from fish and sea water, and 1/10 (10%) for isolates from river water. 2). Plasmid DNA was detected in eight of the 40 isolates examined, but the presence of plasmid did not correlate with enteropathogenicity.
    These results indicate that approximately three fourths of the strains isolated from fish and sea water are enteropathogenic, and that the genes controling the enteropathogenicity of this organism probably exist in chromosomal DNA.
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  • Noriaki HIRUTA, Mariko HIMORI, Masako HABUTSU, Noboru OKAMURA, Masayuk ...
    1991 Volume 65 Issue 5 Pages 537-539
    Published: May 20, 1991
    Released: September 07, 2011
    JOURNALS FREE ACCESS
    A lactose slow-fermenting, non motile Escherichia coli strain was isolated from a diarrheal patient returning from Indonesia.
    Examination of virulence of the strain showed that it penetrated into tissue culture cells, and were positive in Sereny test and enzyme-linked immunosorbent assay for detection of enteroinvasive E. coli.
    A large plasmid responsible for virulence was also detected. Thus the strain was confirmed to be a typical enteroinvasive E. coli.
    Analysis of O serotype using the antisera against E. coli O1-O170 revealed that the strain had O121 antigen, whose antiserum is not included in the commercial serotyping kit for the diagnosis of diarrheagenic E. coli.
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  • Masako TOBITA, Osamu NAKAGOMI, Shiro UESUGI
    1991 Volume 65 Issue 5 Pages 540-545
    Published: May 20, 1991
    Released: September 07, 2011
    JOURNALS FREE ACCESS
    As a result of recent development in medical practice including use of new antimicrobial agents, coagulase-negative Staphylococci (CNS) that were once considered nonpathogenic contaminants have captured attention as causes of disease. The 43 clinical isolates of Staphylococcus epidermidis sensu stricto and 7 isolates of S. epidermidis from the medical staffs were characterized with regard to (1) their biochemical profiles, (2) slime productivity, (3)β-lactamase productivity and (4) plasmid patterns. Most of the isolates have an identical biochemical profile code. The slime production was observed in the 9 strains isolated from pleural effusions or the tubes used for pleural drainage. These strains had a similar antimicrobial susceptibility profile that are commonly seen in multi-resistant strains of methicillin-resistant Staphylococcus aureus (MRSA). In addition, most of these strains produced β-lactamase. For the plasmid profiling, we selected 12 isolates that possessed 5 distinct antimicrobial susceptibility profiles. Upon agarose gel electrophoresis, 8 isolates were shown to possess a 1.9 kb plasmid. These 8 isolates had resistance against tobramycin (TOB) and erythromcin (EM). Emergence of S. epidermidis as causes of disease will increasingly necessitate the detailed microbiological characterization of the clinical isolates.
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  • Hiroshi SAKATA, Shizuo MARUYAMA
    1991 Volume 65 Issue 5 Pages 546-551
    Published: May 20, 1991
    Released: September 07, 2011
    JOURNALS FREE ACCESS
    Influences of cefixime and cefaclor, oral antibiotics, were studied in 50 children (age range, 11 months to 13 years) with pharyngitis. Daily doses of cefixime were 6 to 10 mg/kg in 25 children and those of cefaclor were 40 to 50 mg/kg in 25 children. Pharyngeal swabs were taken before and after 3 to 7 days of the antibiotic treatment. Pathogenic organisms, such as Streptococcus pyogenes, Haemophilus influenzae and Streptococcus pneumoniae, were isolated in 14 children before and in 1 child after cefixime use. But these bacteria were isolated in 15 children before and in 9 children, and 3 strains of H. influenzae and 3 strains of S. pneumoniae were newly isolated after cefaclor use. An isolation rate of Gram-negative bacilli, such as Acinetobacter spp., were increased after use of cefixime. In both antibiotics, isolation rates of yeasts were slightly increased. An isolation rate of alpha-hemolytic streptococci was increased from 16% to 48% by cefixime treatment and from 24% to 52% by cefaclor treatment. The MIC50 of cefixime and cefaclor against alpha-hemolytic streptococci isolated after treatment was higher than that isolated before. Effects of cefixime and cefaclor was less extensive than that of ampicillin in previous reports.
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  • Kenji TAKAGI, Yasutaka YAMASHITA, Hiroo INOUYE, Mitsuaki OHSETO, Hirok ...
    1991 Volume 65 Issue 5 Pages 552-558
    Published: May 20, 1991
    Released: September 07, 2011
    JOURNALS FREE ACCESS
    We were prepared three monoclonal antibodies in which the monoclone 12D was type specific for Adenovirus 40 (Ad40), 1F was type specific for Ad41 and 15D was group specific for Ads. For identification of enteric adenoviruses (EAd) in stool specimens, enzyme-linked immunosorbent assay (ELISA) test using monoclonal antibodies was developed. Results of identification by the ELISA tests using monoclonal antibodies to EAd on 15 fecal samples in which Ad particles were found by electron microscopy showed complete coincidence to those of Sma 1 restriction endonuclease cleavage. From these results, the ELISA tests employing EAds type specific monoclonal antibodies proved to be specific and this was a rapid technique for laboratory diagnosis of EAd in fecal specimens of viral gastroenteritis.
    Fifty-eight fecal samples with Ad particles positive by EM were serotyped by the ELISA using monoclonal antibodies. Eleven fecal samples were identified as Ad40, 25 as Ad41, 1 as double infection with Ad40 and Ad41, and 4 as non-EAd.
    These results indicated that Ad41 was more dominant than Ad40 during April, 1986 to January, 1989 in Matsuyama city.
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  • Yoshiro MORIKAWA, Shigeki TABA, Hisao NAKANISHI, Tadashi TERAMOTO, Min ...
    1991 Volume 65 Issue 5 Pages 559-563
    Published: May 20, 1991
    Released: September 07, 2011
    JOURNALS FREE ACCESS
    In the fall of 1988, an outbreak of streptococcal infections was observed at 2 pediatric clinics in Sanda City, Hyogo prefecture. The 2 clinics were independent of each other; one (clinic A) was located in the down town area, in the older part of the city, where there is little population turn over, while the other (clinic B) was in a newly developed-fast growing residential district.
    The strains and the distribution of T-serotypes isolated at each location are as follows: clinic A; 58 strains (34.5% serotype T-4, 31.0% serotype T-12, 10.3% serotype T-1, and 23.3% serotype T-28), clinic B; 43 strains (48.8% serotype T-28, 23.3% serotype T-12, 11.6% serotype T-4 and 7.0% serotype T-1). According to the data from Kobe City infectious disease surveilance center, there were a total of 102 group A hemolytic streptococci strains isolated in 1988, the T-serotypes distribution of which was as follows: 47.0% serotype T-4, 15.7% serotype the T-12, 10.8% serotype T-1 and 9.8% serotype T-28. Serotype T-4 was dominant here, as it was in location A of Sanda City. The epidemic proportion of serotype T-28 found at location B is considered to be due to the fact that location B has very little social interaction with location A and other areas.
    No difference was observed among the different serotypes on the drug susceptibility test: all strains showed a sensitivity to ampicillin and penicillin G, but were resistant to tetracycline and chloramphenicol.
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  • Hiroshi HUKUHARA, Yoshiteru SHIGENO, Atsushi SAITO
    1991 Volume 65 Issue 5 Pages 564-570
    Published: May 20, 1991
    Released: September 07, 2011
    JOURNALS FREE ACCESS
    We measured serum levels of IgG subclasses in 100 healthy adult humans and 64 patients with respiratory infections by utilizing the enzyme linked immunosorbent assay (ELIZA). The patients were composed of 18 patients with acute bacterial pneumonia and bacterial infection of 9 patients with pulmonary emphysema, 27 patients with chronic bronchitis and 10 patients with bronchiectasis.
    In healthy adults, serum levels of IgG1 subclass decreased in proportion of age increase. Serum levels of IgG2 rose after 30 years of age. Serum levels of IgG3 and IgG4 showed no remarkable changes by age.
    In patients with respiratory infectious disease, serum levels of IgGl, IgG2 and IgG4 decreased significantly but IgG3 increased significantly.
    We also measured serum levels of IgG subclasses in 10 patients with acute bacterial pneumonia and bacterial infection of 5 patients with pulmonary emphysema, 12 patients with chronic bronchitis and 4 patients with bronchiectasis at both infected and convalescent phases. The serum levels of IgG2 in patients with pneumonia and pulmonary emphysema at convalescent phase were significantly lower than those in the patients of infected phase. Other subclasses showed no significant change.
    We summerized that IgG2 was consumed at the infected phase by protecting against bacterial infections. IgG2 probably has an important role of protecting against bacterial respiratory infections among all IgG subclasses.
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  • Makoto SAITO
    1991 Volume 65 Issue 5 Pages 571-576
    Published: May 20, 1991
    Released: September 07, 2011
    JOURNALS FREE ACCESS
    The excretion in the feces of Clostridium perfringens producing enterotoxin by 48 patients in 3 cases of food poisoning was studied during the illness and 1 and 3 months after the onset. Patients in the first outbreak excreted such strains mostly in the form of spores. In the second outbreak, spores and viable count cells were excreted in nearly equal numbers, and about 50% of the spores were enterotoxigenic. In the third outbreak, the strains were detected both as spores and as viable count cells during the illness. In all three groups, most fecal samples contained at least 103 spores per gram. About 1 month after the onset of the illness, (long after recovery from symptoms), 12 colonies producing enterotoxin were detected from five of the 22 samples of feces obtained from subjects in the three groups. There were few C. perfringens organisms, mostly spores, in the feces. Enterotoxin was not detected in the feces. About 3 months after onset, colonies producing enterotoxin could not be detected from feces of 11 of the subjects in the first group. We conclude that persons affected by C. perfringens food poisoning might contaminate food for at least 1 month after the onset.
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  • Michiyasu YAGURA, Hiroshi KAMITSUKASA, Hideharu HARADA, Akira OHBAYASH ...
    1991 Volume 65 Issue 5 Pages 577-585
    Published: May 20, 1991
    Released: September 07, 2011
    JOURNALS FREE ACCESS
    Chinese Hamster Ovary Cell derived HB vaccine (CDV) and plasma.derived HB vaccine (PDV) were seperately given to two groups of 131 and 112 medical stuff subjected, and both the anti-HBs responses were observed for 24 months from the time of first injection. The anti-HBs positive rate at the 7th month was 97%(mean geometric anti-HBs consentration 588 IU/L) in CDV group and 78%(83 IU/L) in PDV group, and the positive rate and mean titer of anti-HBs were always higher for 24 months in CDV group. The differences were remarkable between both the subjects aged over 40 years. The decreasing curves of the anti-HBs titer in the two groups were almost parallel; accordingly, the protective efficacy was estimated to be far longer in CDV. Also, the rate of non-responder was less lower (2.8%) in CDV group than that (15.2%) in PDV group. Above results show that CDV has a higher immunogenicity than PDV.
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  • Hisashi FUNADA, Shosaku FUJII, Toshihiko MACHI, Tamotsu MATSUDA, Akita ...
    1991 Volume 65 Issue 5 Pages 586-590
    Published: May 20, 1991
    Released: September 07, 2011
    JOURNALS FREE ACCESS
  • Shoichiro NAGASAKA, Yatsuka IMAGAWA, Michisato MURATA
    1991 Volume 65 Issue 5 Pages 591-596
    Published: May 20, 1991
    Released: September 07, 2011
    JOURNALS FREE ACCESS
    It has been known that tsutsugamushi disease, so-called “Shichito-fever”, is widely spread among the Izu Islands, Tokyo Metropolis. The cases were reported in Oshima Island, Niijima Island, Shikine Island, Kozu Island, Miyake Island, Mikura Island, and Hachijo Island previously, although no case has been reported in Toshima Island. In this paper, we report the first two cases of tsutsugamushi disease found in Toshima Island in December 1988 and December 1989.
    The first case was a 73 year-old male and the second case was a 83 year-old female, respectively. Fever, erythema and eschar were observed in both cases, while lymphadenopathy and hepatosplenomegaly were not detected. After tetracycline was administered, the fever immediately went down and erythema gradually disappeared in both cases. Specific immunofluorescence tests demonstrated that IgG antibody titers rose against Karp, Gilliam and Kato strains, and that IgM antibody titers rose only against Gilliam strain in both cases. Therefore, Gilliam-like strain of Rickettsia tsutsugamushi may play an important role in Toshima Island.
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  • Haruo MURAOKA, Michio SATA, Teruko HINO, Tatsuya IDE, Seiji NOGUCHI, S ...
    1991 Volume 65 Issue 5 Pages 597-603
    Published: May 20, 1991
    Released: September 07, 2011
    JOURNALS FREE ACCESS
    We had 11 patients with rubella infection accompanied by hepatic dysfunction from 1979 through 1989. The elevations of sGOT were moderate, but sLDH levels were high. Extra hepatic manifestations were suspected by analysis of LDH isozymes. In 9 out of 11 patients, the sGPT levels returned to normal within 8 weeks. But prolonged courses of abnormal transaminase levels were observed in 2 patients. One was accompanied by underlying chronic hepatitis type non-A, non-B, and the other was supposed to have a prolonged rubella infection. Liver biopsy specimen taken from one patient in the acute phase demonstrated a non-specific reactive hepatitis. These observations suggest that rubella infection should be taken into consideration in making the diagnosis of sporadic hepatitis type non-A, non-B.
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  • Hisashi FUNADA, Yasuhiro MOCHIZUKI, Toshihiko MACHI, Shigeki OHTAKE, T ...
    1991 Volume 65 Issue 5 Pages 604-607
    Published: May 20, 1991
    Released: September 07, 2011
    JOURNALS FREE ACCESS
  • Hisashi FUNADA, Kei-ichi MIZUHASHI, Toshihiko MACHI, Shigeki OHTAKE, T ...
    1991 Volume 65 Issue 5 Pages 608-611
    Published: May 20, 1991
    Released: September 07, 2011
    JOURNALS FREE ACCESS
  • Masami SHIMADA, Yuji SAEKI, Shiho MATSUMOTO, Yohnosuke UEMURA, Tomio K ...
    1991 Volume 65 Issue 5 Pages 612-615
    Published: May 20, 1991
    Released: September 07, 2011
    JOURNALS FREE ACCESS
    A 26-year-old housewife was admitted to our hospital with a history of high fever after previous cesarean delivery. She had premature rupture of the membrane on the 41st week of pregnancy and the amniotic fluid was found to be cloudy on the fourth day after rupture. Therefore, cesarean delivery was performed. On the first day of operation, her body temperature increased up to 38°C in spite of the treatment with Latamoxef (LMOX), 3 g/day. A sample of intrauterine material yielded M. hominis in pure culture. After administration of Minocycline (MINO) with antimycoplasmal activity, the clinical symptoms improved by the 11th day of operation.
    Sera obtained after the infection showed antibodies to M. hominis in ELISA study.
    These results suggested that the cause of this postpartum fever was M. hominis infection.
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