Kanzo
Online ISSN : 1881-3593
Print ISSN : 0451-4203
ISSN-L : 0451-4203
Volume 24, Issue 6
Displaying 1-20 of 20 articles from this issue
  • Nobuhito KITAZUME
    1983 Volume 24 Issue 6 Pages 581-590
    Published: June 25, 1983
    Released on J-STAGE: July 09, 2009
    JOURNAL FREE ACCESS
    The author attempted to study the normal architecture of capsular lymph vessels of the liver by means of injection method.
    238 autopsied livers were submitted for this study. India-ink or barium gelatin solution was injected directly into fine lymph vessels in the hepatic capsule. The results obtained were as follows: (1) The capsular (subserosal) lymphatics of the human liver was divided into three categories, i.e., hepatic lymph capillaries, hepatic lymph vessels, and hepatic lymph truncus. The latter two had the valve structure. (2) The hepatic lymph capillaries formed very fine mesh-works in the subserosal connective tissue and about 100 lymph capillary loops covered the surface side of an individual liver lobule. These hapatic lymph capillaries were converging into the collecting tube of hepatic lymph capillaries and draining into the hepatic lymph vessel. (3) The hepatic lymph vessels, in which many collecting tubes of hepatic lymph capillaries drained, run serpentiformly in the capsular connective tissue. Abundunt anastomoses were revealed between the each other. (4) The hepatic lymph truncus corresponded to the duct converging many hepatic lymph vessels and leading hepatic lymph to the extrahepatic lymph route. (5) Eight groups of the hepatic lymph truncus on the anterior upper surface of the liver and six groups on the inferior (visceral) surface were determined. Outflow routes of each hepatic lymph truncus group were described in detail.
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  • Satoshi HASUMURA, Seishi NAGAMORI, Haruo KAMEDA, Hitoshi ENDOU
    1983 Volume 24 Issue 6 Pages 591-597
    Published: June 25, 1983
    Released on J-STAGE: July 09, 2009
    JOURNAL FREE ACCESS
    Micromethods for preparing microsomal fraction and for quantitative estimation of cytochrome P450 (P450) were developed by using experimental animals in order to clarify a possibility for measurering multiple functional properties in ultra small liver biopsy specimen (ca 5mg) which remain after histilogical examination.
    We were able to observe endoplasmic reticulums and ribosomes in the microsomal fraction using an electron microscope. And we were able to measure less than 100 femto moles of P450 with our method. The standard line of P450 was straight under 400 femto moles, whose correlation coefficient (r) was 0.975.
    In some cases of liver diseases, contents of P450 were deviated from 0.1 to 0.3nmoles/mg microsomal protein. Especially, the specimen of liver cirrhosis contained (0.09nmoles/mg microsomal protein) the least amount of P450 and the specimen of chronic hepatitis contained (0.25nmoles/mg microsomal protein) the most in these cases.
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  • A pathological study by electron microscope
    Mitsuo KANAOKA
    1983 Volume 24 Issue 6 Pages 598-607
    Published: June 25, 1983
    Released on J-STAGE: July 09, 2009
    JOURNAL FREE ACCESS
    In this study, interlobular bile ducts and bile ductules observed in 18 specimens from 17 cases with acute viral hepatitis were investigated by electron microscope.
    In most bile ducts seen intact under light microscope "dark cell" and/or "light cell" metamorphosis of bile duct epithelium were observed. Defective basement membrane were observed in the bile ducts where the "dark cell" had spilled out from the basal side and severe cell infiltration occurred only in the specimens of hepatitis A. The organelles exhibited no remarkable changes and the relationship between pinocytotic vesicles and cell infiltration into or around the bile ducts was not apparent. A specific finding with hepatitis A was that macrophages infiltlrated the bile ducts. Virus-like particles were observed in the lysosome of the macrophage which infiltrated the bile duct of a patient with hepatitis A.
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  • A clinicopathological study by light microscope
    Mitsuo KANAOKA
    1983 Volume 24 Issue 6 Pages 608-614
    Published: June 25, 1983
    Released on J-STAGE: July 09, 2009
    JOURNAL FREE ACCESS
    In this study, interlobular bile ducts observed in 55 biopsy specimens taken from 48 patients with acute viral hepatitis on 47.6±17.9 days after onset were investigated.
    The incidence of bile duct lesions was, in order of occurrence, Cell Vacuolation, Nuclear Pleomorphism, Cell Swelling, Karyorrhexis, Nuclear Hyperchromatism, Inflammatory Cells, and Stratification. Bile duct lesions were observed in 18% of all biopsy specimens, and the incidence rate was hepatitis A (26%), non-A, non-B (19%), and B (10%). There was no apparent correlation between bile duct lesion types and the types of acute viral hepatitis. In the specimens taken within 26 days after the disappearance of jaundice, the incidence of bile thrombi was higher (p<0.05) in hepatitis A than in hepatitis B. The relationship between bile duct lesions and bile thrombi was not apparent, and the significance of bile duct lesions has not yet been clarified.
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  • Yoshikazu YONEI, Hiromasa ISHII, Masatoshi KUSUHARA, Go WAKABAYASHI, T ...
    1983 Volume 24 Issue 6 Pages 615-619
    Published: June 25, 1983
    Released on J-STAGE: July 09, 2009
    JOURNAL FREE ACCESS
    The markers for hepatitis B virus including HBsAg and anti-HBs were determined in 484 sera obtained from 300 Japanese and 184 Bolivians living in the San Juan district in the Republic of Bolivia. In adults (older than 16 years old) HBsAg was detected in 7.1% of Japanese, but was not detected in Bolivians. As for anti-HBs, 45% of Japanese were positive, whereas 27% of Bolivian were positive. Among children (undre 16 years old), prevalence of HBsAg was 10% in Japanese and none in Bolivians, whereas anti-HBs were detected in 66% of Japanese and in 3.8% of Bolivians.
    Moreover, twenty six sera obtained from HBsAg positive subjects were tested for HBe antigenantibody system by radioimmunoassay. In adults, 9.1% were positive for HBeAg, whereas 53% of children was HBeAg positive.
    Twenty five HBsAg-positive subjects were also tested for subtypes of HBsAg by hemagglutination inhibition method, revealing that 80% of HBsAg was subtype 'adw', and 20% of it was subtype 'adr'.
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  • Kochi KANAI, Katsutoshi TAMAKOSHI, Makoto KAKO, Akira TAKEHIRO, Mitsuh ...
    1983 Volume 24 Issue 6 Pages 620-626
    Published: June 25, 1983
    Released on J-STAGE: July 09, 2009
    JOURNAL FREE ACCESS
    The efficacy of anti-HB immune globulin and HB vaccine for the prevention of vertical transmission of HB virus to babies born to HB carrier mothers has been investigated.
    Thirty eitht babies bom to HBe antigen-positive mothers received 150mg of HBIG·F (ab')2 intravenously within two hours after delivery and serum anti-HBs antibody titer (PHA) was kept higher than 222 by subcutaneous injection of HBIG thereafter. The interval between each HBIG injection was three months in most cases. The administration of HB vaccine was started at three months after delivery. Three injections of HB vaccine were given to the babies at one month interval. It has been disclosed that all babies who were injected HBIG remained HBsAg-negative except for two cases who became HBs-Ag-positive shortly after birth.
    About fifty percent of children became actively immunized after three dosis of HB vaccine and at present, 78.3% of the babies have obtained active immunization with mean injection of 3.4 dosis of HB vaccine.
    No side effect has been observed by the administration of either HBIG or HB vaccine.
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  • Including the comparison of the two kinds of perfusion studies
    Takeshi OKANOUE, Ongyoku OU, Jun YOSHIDA, Masahara OHTA, Takeshi OGASA ...
    1983 Volume 24 Issue 6 Pages 627-632
    Published: June 25, 1983
    Released on J-STAGE: July 09, 2009
    JOURNAL FREE ACCESS
    In order to examine the cytoskeleton in normal rat hepatocyte and the effect of acute ethanol administration on the cytoskeleton of rat hepatocyte rat livers were perfused with 0.5% Triton X-100 or 0.15% Triton X-100 for 120min. In ethanol group rat 5g/kg b.w. ethanol were given intragastrically. Microtubles and intermediate filaments were easily observed by this two kinds of perfusion studies; however, it was difficult to observe microfilament in this two kinds of perfusion studies. No significant morphological changes were noted in the microtubles and the intermediate filaments in the rat hepatocytes received acute ethanol administration compared with the control rats.
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  • Masaru MIYAZAKI, Shigeru FUJIMOTO, Takenori SHIMURA, Osamu TAKAHASHI, ...
    1983 Volume 24 Issue 6 Pages 633-640
    Published: June 25, 1983
    Released on J-STAGE: July 09, 2009
    JOURNAL FREE ACCESS
    This study presents a model for the in vivo perfusion of the isolated rat liver with either cytotoxic agents or hyperthermic saline administered either through the hepatic artery or the portal vein.
    Rats were able to survive following the perfusion with a dose of up to 0.25g/kg of 5-FU and 2.0-2.5mg/kg of Mit-C by both routes.
    Regional hyperthermic hepatic perfusion produced temperature- and time-dependent hepatotoxic effects. A marked elevation in SGOT and SGPT levels was observed 24 hours after perfusion, but was reversible.
    3H-TdR incorportion into hepatic DNA 24 hours after partial hepatectomy was severely inhibited by prior hepatic perfusion with either cytotoxic drugs or regional hyperthermia. This in vivo isolated hepatic perfusion technique could be applicable for assessing the effect of thermochemotherapy on tumor growth in the liver.
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  • A study of tumor thrombus of the portal vein
    Atsuo JIMI
    1983 Volume 24 Issue 6 Pages 641-647
    Published: June 25, 1983
    Released on J-STAGE: July 09, 2009
    JOURNAL FREE ACCESS
    Tumor thrombus (TT) was studied histologically in 95 livers bearing hepatocellular carcinoma (HCC). TT was seen in 75 out of the 95 cases (78.9%). Four growth patterns, arbitrarily described as "proliferative type" (35 cases), "necrotic type" (14 cases), "mixed proliferative and necrotic type" (21 cases) and "organized type" (5 cases), were distinguished. Besides tumor extension into the intrahepatic portal vein branches, tumor grows into the periportal capillaries and periductal capillary plexuses as well.
    Branches of the hepatic artery are frequently engulfed by the tumor, and it is common to find aneurysmal change and its rupture which cause abnormal shunt formation among the hepatic artery, the portal vein and tumor vessel. Organization of TT appears in the subendothelial space with an increase of collagen fibers. Narrowing and/or obstruction of the portal vein by TT and abnormal shunt formation between the hepatic artery and the portal vein may accelerate a portal hypertension. Thus, risk of rupture of esophageal varices may increase in HCC patients.
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  • Kenji HIRAI, Masanobu KUMAGAI, Tamotsu SUKO, Taisuke SAKEMI, Yoshikazu ...
    1983 Volume 24 Issue 6 Pages 648-653
    Published: June 25, 1983
    Released on J-STAGE: July 09, 2009
    JOURNAL FREE ACCESS
    The changes of angiographic findings such as tumor size, diameter of the proper hepatic artery (PHA), displacement of the artery, A-V shunt, intrahepatic metastatic lesions, hypervascularity and tumor stain were assessed before and after one-shot injection of anticancer agents via the hepatic artery in 55 cases of unresectable hepatocellular carcinoma (HCC).
    The results were shown as follows:
    1) A high correlation was noted between the caliber of the PHA and the change in tumor size.
    2) Typical angiographic findings of HCC such as hypervascularity, tumor stain, displacement, A-V shunt and metastatic lesions were also changed with tumor size.
    3) Angiographic changes did not show to be correlated with tumor effects in aggravated cases after chemotherapy. The caliber of PHA decreased or unchanged in 30%, and the degree of hypervascularity or tumor stain unchanged in 90% cases in spite of tumor enlargement.
    These findings described above suggest that in some cases, histological changes after chemotherapy can be assessed on angiography.
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  • Hisao HAYASHI, Kazuhiko HUKUI, Hiroshi MURAKAMI, Junsuke KURIKI, Shini ...
    1983 Volume 24 Issue 6 Pages 654-658
    Published: June 25, 1983
    Released on J-STAGE: July 09, 2009
    JOURNAL FREE ACCESS
    A 16 year-old boy was found to have elevated levels of serum transaminases in the screening test of blood donor. He was asymptomatic and family history was negative for liver or neuropsychiatric disorder.
    Further examination disclosed a characteristic feature of copper metabolism to Wilson's disease: hypocupremia, hypoceruloplasminemia and increased urinary excretion of copper. But Kayser Fleischer ring and extrapyramidal symptoms were not recognized. The histology of liver consisted with chronic active hepatitis with fatty metamorphosis and glycogen nuclei. Copper concentration was high as 1025μg per gram dry liver weight. A final diagnosis of asymptomatic Wilson's disease was made and D-penicillamine therapy was instituted uneventfully.
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  • Tomoki ARITAKA, Michio SATA, Naoto MARUYAMA, Hiroshi SETOYAMA, Fumiyuk ...
    1983 Volume 24 Issue 6 Pages 659-663
    Published: June 25, 1983
    Released on J-STAGE: July 09, 2009
    JOURNAL FREE ACCESS
    An unusual case of acute hepatitis A, associated with muscular involvement, was reported.
    A 34-year old male was admitted with a high fever, myalgia of extremities and jaundice.
    Laboratory studies disclosed markedly elevated serum transaminase, lactic dehydrogenase and creatinine phosphokinase activites. Serum IgM anti HAV was positive. Serum creatinine phosphokinase level turned to normal values along with the normalization of serum transamirase and lactic dehydrogenase levels. Liver biopsy performed at 14th clinical day showed minimal changes, althouth serum transaminase activity was markedly elevated.
    Circulatory immune complex (cIC) was measured by Raji cell assay method. IgA and IgM class cIC were detected at acute stage except for IgG class cIC. In this case unlike usual cases with acute hepatitis A, both of IgA and IgM class cIC were detected even at the convalescent stage. These findings suggested the possible role of IgA or IgM class cIC in the pathogenesis of this muscular involvement.
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  • Shinzo KATO, Hiromasa ISHII, Toshikazu TAKAGI, Yoshiaki ASABA, Makoto ...
    1983 Volume 24 Issue 6 Pages 664-668
    Published: June 25, 1983
    Released on J-STAGE: July 09, 2009
    JOURNAL FREE ACCESS
    A 43 year-old male, who has no complaints, was admitted for the evaluation of the persistent elevation of serum Glutamic Oxaloacetic Transaminase (GOT) activity. He showed slight hepatomegaly, but no other abnormal findings in physical examination. The abnormal laboratory finding was found only in serum GOT level, and other tests including ECG, EMG, ICG-15 min, liver scintigram and histology of biopsied liver were normal.
    By using electrophoresis and immuno-fixation technique, we found that serum GOT was bound to immunoglobulin Ig-G (λ) and forrned enzyme-immunogolbulin complex. Elevation of serum GOT seemed to be derived from the delayed disappearance rate of GOT from the blood, and not from the increase in release of GOT from the tissues.
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  • Atsuyoshi ONITSUKA, Takashi HIRAI, Shigefumi KOIKE, Ryosho TOMITA, Aki ...
    1983 Volume 24 Issue 6 Pages 669-674
    Published: June 25, 1983
    Released on J-STAGE: July 09, 2009
    JOURNAL FREE ACCESS
    A 61-year-old man who had hepatoma in the right lobe with liver cirrhosis underwent right hepatic lobectomy on June of 1980. Pathohistologically, the surgical specimen showed combined liver and bile duct carcinoma. Eighteen months later, he underwent partial hepatectomy of left lateral segment for the recurrence of hepatoma. This tumor was regarded as a multicentric carcinogenesis on account of pathohistology which showed clear cell type hepatoma. Further nine months later, he underwent third hepatectomy consisting of two partial resection of left lateral and medial segments for the recurrence in each segment. These tumors were regarded as a recurrence of hepatoma resected at the first operation because of pathohistology which showed combined liver and bile duct carcinoma. He is alive and well two years and five months after the first right hepatic lobectomy.
    A good result by hepatectomy for the recurrent hepatoma which was considered before to be noncurative is expected.
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  • [in Japanese], [in Japanese]
    1983 Volume 24 Issue 6 Pages 675
    Published: June 25, 1983
    Released on J-STAGE: July 09, 2009
    JOURNAL FREE ACCESS
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  • [in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    1983 Volume 24 Issue 6 Pages 676
    Published: June 25, 1983
    Released on J-STAGE: July 09, 2009
    JOURNAL FREE ACCESS
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  • [in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    1983 Volume 24 Issue 6 Pages 677
    Published: June 25, 1983
    Released on J-STAGE: July 09, 2009
    JOURNAL FREE ACCESS
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  • [in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    1983 Volume 24 Issue 6 Pages 678
    Published: June 25, 1983
    Released on J-STAGE: July 09, 2009
    JOURNAL FREE ACCESS
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  • [in Japanese], [in Japanese], [in Japanese]
    1983 Volume 24 Issue 6 Pages 679
    Published: June 25, 1983
    Released on J-STAGE: July 09, 2009
    JOURNAL FREE ACCESS
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  • 1983 Volume 24 Issue 6 Pages 680-698
    Published: June 25, 1983
    Released on J-STAGE: July 09, 2009
    JOURNAL FREE ACCESS
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