Kanzo
Online ISSN : 1881-3593
Print ISSN : 0451-4203
ISSN-L : 0451-4203
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Volume 32, Issue 5
Displaying 1-20 of 20 articles from this issue
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  • Detection of antibodies to hepatitis C virus in patients with non-A, non-B liver disease
    Nobukazu YUKI, Norio HAYASHI, Akinori KASAHARA, Hideki HAGIWARA, Keiji ...
    1991 Volume 32 Issue 5 Pages 465-470
    Published: May 25, 1991
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.32.465
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    Antibodies to hepatitis C virus (HCV) were determined by solid-phase enzyme immunoassay using recombinant C100-3 antigen and a variety of synthetic peptides corresponding to the polypeptide coded for by HCV genome. In acute non-A, non-B hepatitis, anti-peptide antibodies were detected in five (42%) of 12 patients. Anti-C100-3 antibodies were detected in three patients positive for anti-peptide antibodies. In chronic non-A, non-B liver disease, anti-peptide antibodies were detected in anti-C100-3-negative patients (7/15, 47%) as well as in anti-C100-3-positive patients (19/31, 61%). Thirty-seven chronic hepatitis B virus carriers negative for anti-C100-3 antibodies were also tested for anti-peptide antibodies. Of these, two (5%) were positive for anti-peptide antibodies, and the positive rate was lower than in patients with chronic non-A, non-B liver disease. These results indicate that immune responses can be directed toward various regions of HCV polypeptide and HCV infection may be involved even in patients negative for anti-C100-3 antibodies.
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  • Proliferation of duck hepatitis B virus in primary duckling hepatocyte cultures
    Shintaro ABE
    1991 Volume 32 Issue 5 Pages 471-477
    Published: May 25, 1991
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.32.471
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    It has been known that duct hepatitis B virus (DHBV), which is transmitted in vivo or in vitro, proliferated in the primary hepatocyte cultures of ducklings. Following findings were obtained from the present experiment, utilizing primary hepatocyte cultures; first, the hepatocyte cultures were maintained for one month and DHBV continued to multiply steadily through this period both in vivo and in vitro transmission; second, the successful in vitro infection of DHBV was recognized up to 21 days so far examined, where DHBcAg-positive cells were approximately 10% irrespective to the time of transmission. Thus DHBV was able to infect the primary cultured hepatocytes even when they exhibited spindle shape alternation; third, there was no remarkable difference in the multiplication of DHBV on individual infected cell level between in vivo and in vitro transmission; fourth, the administration of dimethyl sulfoxide (DMSO) and N6-2'-0-dibutyryl-adenosine3': 5'-cyclic monophosphate (dbc-AMP) prevented the cultured cells from changing to a spindle shape to some extent, but did not affect the infection and proliferation of DHBV.
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  • Extrahepatic portovenous shunts in asymptomatic primary biliary cirrhosis
    Shuichi SEZAI, Shin SAKURABAYASHI, Yoshihiro YAMAMOTO, Kenta YOSHIURA, ...
    1991 Volume 32 Issue 5 Pages 478-482
    Published: May 25, 1991
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.32.478
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    Extrahepatic portovenous shunts were demonstrated in 4 of the 5 patients with asymptomatic primary biliary eirrhosis (a-PBC) (3 gastro-renal shunts, and one each of spleno-renal and retroperitoneal shunts). We found no statistical differences in the results through comparative studies between a-PBC patients and control subjects in regard to portal venous blood flow (PVf). Portal venous pressure was within normal limits in 4 of the 5 studied patients. A presinusoidal block was histopathologically recognized in 2 of the three without portal hypertension, while a sinusoidal change was extensively noted in the remainder with portal hypertension. In conclusion, the extrahepatic portovenous shunt is formed in the very early stage of PBC along with other histopathological changes.
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  • A clinical study on all-night polysomnography in patients with chronic liver failure
    Masahiko MURASE, Yasutoshi MUTO, Koji KITAGAWA, Koshiro SAITO, Hisatak ...
    1991 Volume 32 Issue 5 Pages 483-491
    Published: May 25, 1991
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.32.483
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    A study was conducted to elucidate the pattern of impaired sleep, such as a night-day reversal of sleep cycle, often observed in chronic liver failure. Twelve recordings of all-night polysomnography were performed in 8 patients, and the following results were obtained:
    1) In 6 patients without encephalopathy at examination, significant reductions in total sleep time (TST), sleep stage 2 and, in particular, REM (rapid eye movement) sleep were observed, while stage W (awaken) was significantly increased when compared with age-matched healthy volunteers. No changes were noted in deep slow wave sleep (SWS), sleep latency and REM latency, and sleep cycle was well preserved.
    2) Abnormalities in laboratory data representing elevated blood ammonia level and reduced plasma BCAA/AAA molar ratio were still observed in those patients without hepatic encephalopathy.
    3) In one patient who was treated with BCAA granules, decreased REM sleep was found to recover toward normal in parallel with improvement of laboratory data.
    These results suggest that abnormal sleep patterns observed in chronic liver failure are reversible by appropriate treatments, and that changes in brain neurotransmitters, including monoamines which are closely related with sleep control, have already occurred at the period free from hepatic encephalopathy.
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  • The effect of partial hepatectomy and/or splenectomy on the appearance of phenotypic expressions of γ-glutamyltranspeptidase and c-myc oncoprotein during hepatocarcinogenesis in rats
    Masatoshi NAKATA, Akiyoshi KASHII, Takao SAKAI, Norio HIROTA
    1991 Volume 32 Issue 5 Pages 492-497
    Published: May 25, 1991
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.32.492
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    We examined the effect of partial hepatectomy (PH) and/or splenectomy (SPX) during diethyl-nitrosamine-induced hepatocarcinogenesis in rats by sequential, quantitative analysis of γ-glutamyl-transpeptidase (GGT)-positive hepatocellular foci. Immunohistochemical expression of c-myc protein which is known to be closely related to cellular proliferation was additionally investigated along with cellular glycogen-storage abnormality. GGT-positive foci increased in number in a group of PH compared with non-PH groups, but they were most pronounced in a group of PH plus SPX at the experimental weeks of 36. c-myc-positive foci, which first appeared in groups of PH and PH plus SPX at 24 weeks, were found to be restricted to glycogen-deficient foci; 81% of glycogen-deficient foci were c-myc positive. The data suggest that PH or PH plus SPX which is performed in the process of hepatocarcinogenesis has a potential to significant enhancement of the (pre-) neoplastic progression.
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  • Polyamine levels and ornithine decarboxylase activity in human hepatocellular carcinoma
    Akihiro TAMORI, Shuhei NISHIGUCHI, Koshun KIM, Hiroko OKA, Shuichi SEK ...
    1991 Volume 32 Issue 5 Pages 498-504
    Published: May 25, 1991
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.32.498
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    Operative specimens from 18 patients with hepatocellular carcinoma (HCC) were separated into cancerous and non-cancerous tissue, and we assayed the tissue for ornithine decarboxylase (ODC) activity and polyamine levels. The mean ODC activity in cancerous tissue was significantly higher than in non-cancerous tissue from the same patient in the group of 12 patients who did not undergo transcatheter arterial embolization (TAE). The concentrations of putrescine (PUT) and spermidine (SPD) in cancerous tissue were significantly elevated, but that of spermine (SPM) was not. ODC activity in cancerous tissue in which cellular atypism was high (n=5), are probably tended to be higher than in the other specimens. In the TAE group, with 6 patients, ODC activity in the non-necrotic cancer tissue was as same as in non-cancerous tissue. The concentrations of PUT, SPD, and SPM in non-necrotic cancerous tissue were the same as those in non-cancerous tissue. These results suggested that polyamine levels were increased by the activation of ODC in cancerous tissue, and TAE reduced ODC activity and polyamine levels in cancerous tissue.
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  • Thermotolerance and heat shock protein 72 (HSP 72) in primary cultured hepatocytes
    Yasuyuki NAGAO, Takeshi OKANOUE, Yoshito ITO, Michio MORIMOTO, Keizo K ...
    1991 Volume 32 Issue 5 Pages 505-511
    Published: May 25, 1991
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.32.505
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    To clarify the changes of stress fibers (SF), HSP 72 and cell viability (CV) by various grades of heat shock (HS) treatment in primary cultured hepatocytes, we applied immunofluorescence for actin filaments and HSP 72, and MTT assay and methylene blue assay to evaluate CV. HS at 43°C and 45°C induced the disappearance of SF before the loss of CV and this phenomenon was reversibly recovered, if the treatment was not lethal. Therefore, the change of SF was able to become a guideline of the reversible cell damage. Both MTT assay and methylene blue assay seemed to have good correlation but the MTT assay showed apparent differences in this study. The primary cultured hepatocytes treated at 43°C for 20min. became gradually thermotolerant and the maximum thermotolerance level was 8 to 12 hours following the conditioning treatment. Our results indicate that HSP 72 accumulated in the nucleoli plays important roles in the thermotolerance.
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  • A case of liver failure due to long-term ingestion of acetaminophen
    Yuriko KOGA, Yutaka NAKASHIMA, Ryukichi KUMASHIRO, Takato UENO, Syotar ...
    1991 Volume 32 Issue 5 Pages 512-515
    Published: May 25, 1991
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.32.512
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    A 33-year-old woman with liver failure associated with the chronic ingestion of excessive dose of acetaminophen (AAF) was presented. For last five years, she had an analgesics containing AAF intermitently because of headache and fret and fume. She continued to take AAF and she frequently took as much as 40 tablets (5300mg AAF) daily. Two days after the last AAF ingestion, she felt abdominal pain and laboratory data revealed severe liver dysfunction. Plasma exchang, glucagoninsulin therapy and administration of glutathione, N-acetylcysteine and cimetidine saved her from liver failure.
    Liver biopsy showed fibrosis and bridging suggesting a chronic liver damage. It was suggested that long-term ingestion of AAF brings the decrease of the amount of glutathion and the induction of cytochrome P-450 oxidizing enzymes, which may lead to an excessive groduction of AAF metabolites as the cause of hepatic failure. A special care should be taken for those with chronic AAF intoxication.
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  • A case of liver cirrhosis with pulmonary arterio-venous shunts manifesting cyanosis after injection sclerotherapy of esophageal varices
    Tsukasa YAMASHITA, Hironori SAKAI, Bunei ANDOU, Shigeru SAKAMOTO, Haji ...
    1991 Volume 32 Issue 5 Pages 516-521
    Published: May 25, 1991
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.32.516
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    In this report, we present a case of liver cirrhosis with arterio-venous shunts in the lung demonstrated by radionuclide method, who manifested cyanosis after injection sclerotherapy of esophageal varices.
    In December. 1985, a 38-year-old male was admitted to a hospital because of liver cirrhosis and esophageal varices with red wale markings, where several times of injection sclerotherapy were undergone until January 1986. In March 1986, he began to complain of exertional dyspnea. In June 1986, he was admitted to our hospital.
    On examination, clubbed fingers were noted and cyanasis was manifested by exercise. Arterial oxygen tension during the breathing of room air was 55 mmHg. Perfusion lung scanning with 99mTc-MAA revealed multiple defects of lung and abnormal hyperradioactivities of brain, thyroid gland and bilateral kidneys. Percent right to left shunt estimated by pulmonary flow through rate of 99mTc-MAA was as high as 73%.
    It was supposed that hypoxemia was worsened because of small embolism of the lung caused by coagulants injected by sclerotherapy.
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  • Aneurysm of the splenic vein complicated with liver cirrhosis
    A case report of long-term observation
    Akinori MATSUMOTO, Motoyuki OOHIRA, Minoru ONO, Masumi KANAI, Hitoyosh ...
    1991 Volume 32 Issue 5 Pages 522-528
    Published: May 25, 1991
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.32.522
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    We reported a rare case of splenic vein aneurysm complicated with liver cirrhosis. A 51-year-old man was admitted to the hospital in August 1985 for the evaluation and treatment of hepatic disease and esophageal varices. Liver biopsy performed under laparoscopic observation led to the diagnosis of liver cirrhosis. Endoscopic injection sclerotherapy was performed for the esophageal varices. Abdominal CT and angiography revealed a splenic vein aneurysm of 20mm in diameter. Hepatic failure progressed, and the development of hepatoma was detected in August 1988. The splenic vein aneurysm gradually increased in size without the complications of thrombosis and rupture. Autopsy done in June 1989 found that the splenic vein aneurysm was expanded to a diameter of 45mm and that its wall was atrophied. Splenic vein aneurysms very rarely occur in the portal system. In addition, the present histopathological findings of atrophic changes found in the aneurysmal wall would provide useful information on the pathogenesis of splenic vein aneurysms.
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  • A case of pediatric primary sclerosing cholangitis
    Hiroki KAKIMOTO, Sumio KAWATA, Kenji TAKAISHI, Toshihiko NAGASE, Nobuy ...
    1991 Volume 32 Issue 5 Pages 529-533
    Published: May 25, 1991
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.32.529
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    A 15-year-old male was diagnosed as primary sclerosing cholangitis (PSC) in an asymptomatic stage. Endoscopic retrograde cholangiography showed narrowing and dilatation of the common hepatic and intrahepatic bile ducts. Histological findings of the liver revealed periductal fibrosis, a characteristic feature of PSC. The liver biopsy spiecemin obtained when the patient was nine years old showed the infiltration of eosinocytes in portal tract without periductal fibrosis, suggesting a relation ta the pathognosis of PSC.
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  • Immunohistological detection of proliferating cells using anti-PCNA monoclonal antibody in paraffin-embedded liver needle-biopsied specimens
    Kazunari TANAKA, Kiwamu OKITA
    1991 Volume 32 Issue 5 Pages 534-535
    Published: May 25, 1991
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.32.534
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  • Clinicopathological study on multicentric carcinogenesis of resected hepatacellular carcinama based on the histologic grade of recurrent nodules obteined by ultrasound-guided fine needle biopsy
    Koji OKUDA, Masatoshi TANAKA, Kazusaburo ANDO, Mikihisa MUTA, Hideki S ...
    1991 Volume 32 Issue 5 Pages 536-537
    Published: May 25, 1991
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.32.536
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    1991 Volume 32 Issue 5 Pages 538
    Published: May 25, 1991
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.32.538
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    1991 Volume 32 Issue 5 Pages 539
    Published: May 25, 1991
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.32.539
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    1991 Volume 32 Issue 5 Pages 540
    Published: May 25, 1991
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.32.540
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    1991 Volume 32 Issue 5 Pages 541
    Published: May 25, 1991
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.32.541
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  • [title in Japanese]
    [in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    1991 Volume 32 Issue 5 Pages 542
    Published: May 25, 1991
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.32.542
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  • [title in Japanese]
    1991 Volume 32 Issue 5 Pages 543-589
    Published: May 25, 1991
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.32.543
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  • Erratum to : Criteria of curability in the hepatectomy for hepatocellular carcinoma
    1991 Volume 32 Issue 5 Pages 590
    Published: 1991
    Released on J-STAGE: July 09, 2009
    DOIhttps://doi.org/10.2957/kanzo.32.5_590
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