Kanzo
Online ISSN : 1881-3593
Print ISSN : 0451-4203
ISSN-L : 0451-4203
Volume 32, Issue 6
Displaying 1-14 of 14 articles from this issue
  • Chikateru NOZAKI, Kyosuke MIZUNO, Mariko ESUMI, Toshio SHIKATA
    1991 Volume 32 Issue 6 Pages 591-595
    Published: June 25, 1991
    Released on J-STAGE: July 09, 2009
    JOURNAL FREE ACCESS
    C8-2 cDNA is a clone coding for a part of NS5 protein of HCV. The protein coded by C8-2 cDNA reacts with sera of patients with HC. We devided C8-2 cDNA into two parts which codes 1-37aa and 38-89aa, respectively. These cDNAs were expressed in E. coli, and both of recombinant proteins derived from these cDNAs reacted with sera of patients with HC. Three synthetic peptides (1-37aa, 38-72aa, 60-89aa) derived from C8-2 region reacted with sera of patients with HC. These results show that more than three epitopes exsist in C8-2 region.
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  • Shusaku NAKATA, Toshiji SAIBARA, Saburo ONISHI, Yasutake YAMAMOTO
    1991 Volume 32 Issue 6 Pages 596-603
    Published: June 25, 1991
    Released on J-STAGE: July 09, 2009
    JOURNAL FREE ACCESS
    The mechanism of biliary epithelial cell injury was examined by assessing spleen-derived lymphocyte cytotoxicity to autologous biliary epithelial cells in patients with primary biliary cirrhosis(PBC). Spleen-lymphocytes had cytotoxicity against autologous biliary epithelial cells in all of 3 patients with PBC but in none of 6 patients with non-PBC. The cytotoxicity was found in T cells butnot in non-T cells. CD8 positive T lymphocytes were shown to be responsible for the cytotoxicity by negative depletion and the cytotoxicity was partially suppressed by anti-MHC class I. It was suggested that T cell cytotoxicity functioned through the interaction with MHC class I antigens. The cytotoxicity was dose-dependently suppressed by anti-biliary epithelial cell antibody. Our data support the current hypothesis that the pathogenesis of PBC is due to T cell autoimmunity against biliary epithelium.
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  • Shinji ISOMURA, Masaaki EBARA, Masao OHTO, Fukuo KONDO, Yasutoshi SUZU ...
    1991 Volume 32 Issue 6 Pages 604-612
    Published: June 25, 1991
    Released on J-STAGE: July 09, 2009
    JOURNAL FREE ACCESS
    A study was made of 102 patients with hepatocellular carcinoma (HCC) 2-10cm in diameter following transcatheter arterial embolization (TAE). It was studied on a long term obsavation. Multivariate analysis proved that the size and the type of tumors were significantly correlated with a prognosis in patients with HCC treated by TAE. Survival rates in the TAE group were evaluated in comparison with those in an untreated group. The groups were previously estimated to be equivalent in the combination of the size and the type of tumors. In nodular typed HCC of 3-5cm and 5-10cm diameter and in massive typed HCC of 5-10cm, the TAE group had a significantly better survival rate than the untreated group. However, the median survival of massive typed HCC of 5-10cm diameter was only 6 months.
    This concluded clinically that nodular typed HCC of 3-10cm responded best to TAE.
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  • Toshihito SEKI, Yoshitsugu KUBOTA, Kouji KUNIEDA, Yoshihiko KANOU, Mas ...
    1991 Volume 32 Issue 6 Pages 613-617
    Published: June 25, 1991
    Released on J-STAGE: July 09, 2009
    JOURNAL FREE ACCESS
    Ultrasonically guided percutaneous ethanol injection therapy (PEIT) was performed in 10 patients with hepatocellular carcinoma (HCC, nodular type, 5-9cm in diameter) in whom neither surgery nor transcatheter arterial embolization (TAE) was carried out. The average number of times of treatment was eight. The average total amount of injected ethanol was 96ml. The survival period of each patient to date was between 7 months and 32 months. In the entireseries, only three patients died as a result of advanced HCC.
    Except for one patient who recieved insufficient treatment due to severe abdominal pain, the ayerage decrease rate of the main tumor after 6 months was 36%. Recurrence was detected in 8 patients between 8 months and 28 months after treatment.
    We performed PEIT for recurrent nodules and were able to supress the recurrent nodules from advancing. Although we used a large dose of ethanol, clinically relevant side effects or complications were not noted and liver function did not worsen. We conclude that PEIT may be a potentially effective treatment for large sized HCC in whom neither surgery nor TAE was applied.
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  • Osamu EDAMITSU, Kazumitsu KIYOMATSU, Osamu NAKASHIMA, Shigetaka SUGIHA ...
    1991 Volume 32 Issue 6 Pages 618-624
    Published: June 25, 1991
    Released on J-STAGE: July 09, 2009
    JOURNAL FREE ACCESS
    Pathomorphologic features of 34 cases of hepatocellular carcinoma (HCC) showing hyperechoic pattern among 88 surgically resected HCCs less than 3cm in diameter were studied. The frequency of hyperechoic tumors by tumor size were 11.1% of the tumor less than 1cm in diameter, 44.7% of the tumor ranging 1.1 to 2.0cm in diameter and 39% of the tumor ranging 2.1 to 3.0cm in diameter. The most common features reflecting hyper echogenicity was fatty change (20 cases) and followed by pseudoglandular HCCs (6 cases), peliotic dilatation of blood space (6 cases), sclerotic HCC (1 case), and clear cell change (1 case).
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  • Makoto OHBU, Masahiko OKUDAIRA, Kiyoharu WATANABE, Tomoko TAKAI, Satos ...
    1991 Volume 32 Issue 6 Pages 625-632
    Published: June 25, 1991
    Released on J-STAGE: July 09, 2009
    JOURNAL FREE ACCESS
    Twenty seven post-mortem and eight biopsied specimens of the liver from patients who had undergone allogeneic renal transplantation were studied. We found a peculiar change of interlobular bile duct, which was characterized by markedly swollen, eosinophilic granular cytoplasm of bile duct epithelium. The finding resembled hepatocytes, thus we called it heptocellular metaplasia of interlobular bile duct epithelium. The epithelium showing hepatocellular metaplasia was PAS-positive and PAS-negative after diastase digestion. In immunohistochemical staining, it demonstrated positive reaction for albumin, whilst, keratin and cytokeratin were negative. Hepatocellular metaplasia was also seen in the liver specimens from some patients with chronic renal failure and chronic active hepatitis B. It was noticed that in renal transplant recipients with hepatocellular metaplasia that a good correlation between hepatocellular metaplasia and elevation of serum γ-GTP was recognized. Hepatocellular metaplasia does not always signify adverse reaction to man, however, it seems to be an interesting finding to us when considering relation between morphological alteration and elevation of enzyme associated with biliary tract.
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  • Tetsuro NAGAOKA
    1991 Volume 32 Issue 6 Pages 633-642
    Published: June 25, 1991
    Released on J-STAGE: July 09, 2009
    JOURNAL FREE ACCESS
    The levels of plasma nucleosides were measured with HPLC in the rats during hepatic regeneration following partial hepatectomy and CCl4-induced acute liver injury. After hepatectomy, cytidine (Cyd) and adenosine (Ado) were significantly increased at three and six hours, preceding the peak of transaminases. At 36, 48, and 72 hours after the operation, Cyd and urindine (Urd) were significantly decreased, while the increase in mitotic index was observed. At 24 hours after administration of CCl4, Cyd, Urd, Ado, pseudouridine (Ψrd), and 1-methyladenosine (m1A) were increased, preceding the elevation of transaminases. At 72 hours, Cyd was significantly decreased, while mitotic index was increased. These results suggest the relationship between reduction of plasma nucleoside levels and hepatic regeneration.
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  • Utilization of Laser cytometer
    Tomoko TACHIBANA, Keiichiro YONEYAMA, Yoshio HATTA
    1991 Volume 32 Issue 6 Pages 643-649
    Published: June 25, 1991
    Released on J-STAGE: July 09, 2009
    JOURNAL FREE ACCESS
    The role of Na+-H+ exchanger in the maintenance of intracellular pH (pHi) was examined with 24 hour-cultured couplet cells isolated from rat liver. The pHi was measured with bis (carboxyethyl)carboxyfluorescein (BCECF) and Laser cytometer, and the recovery rate of the pHi was mesured by the period of time for the acidified cellular pH with NH4Cl to return to pH of the cellular original set point after removal of NH4Cl solution. The recovery was found to be dependent on the presence of extracellular Na+ and it was blocked by amiloride, and Na+-H+ exchanger exhibited a set point. The pHi recovery rate (ΔpH/min) varied with the pHi. The phorbol ester, 12-0-tetradecanoyl-phorbol-13-acet-ate (TPA), increased the recovery rate and shifted pH of set point. The observations indicate that the Na+-H+ exchanger regulates pHi in cultured couplet cells of rat hepatocytes, and the exchanger may act through protein kinase C. Laser cytometer is very useful for determination of pumping function of cell membrane by observation of pHi changes.
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  • Hiromichi FUJIOKA, Kouichi HAMAGUCHI, Makoto OGASAWARA, Hiromi AONUMA, ...
    1991 Volume 32 Issue 6 Pages 650-656
    Published: June 25, 1991
    Released on J-STAGE: July 09, 2009
    JOURNAL FREE ACCESS
    A 70-year-old male was admitted because of general fatigue, itching and jaundice on January 22, '89. Laboratory findings showed that total bilirubin was 21.3mg/dl, TTT was 26.9KU and GOT was 99KU. Serum IgM anti-HAV was positive with 7.8 cut off index. Definite diagnosis of acute hepatitis A was made. On 15th hospital day, hemoglobin decreased to 4.3g/dl with reticulocytes of 0.1%.Total bilirubin was elevated to 23.7mg/dl with 16.6mg/dl of direct bilirubin, and associated with elevation of LDH of 1822WU. Analysis of LDH isozyme showed that LDH1 was 35%. Marked decrease of serum haptoglobin was observed. The finding of bone marrow on 23th hospital day revealed that marked hypoplasia of erythroid series. These results were consistent with pure red cell aplasia (PRCA) accompanied by hemolysis. Three units of washed red blood cell was transfused daily for four days. Oral administration of prednisolone was initiated from 60mg per day. On 73th hospital day the hemoglobin was improved within normal range. The peritoneoscopic finding of the liver and the microscopic finding of the liver biopsy specimen showed intrahepatic cholestasis.
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  • Kazuhiko KITA, Masaaki EBARA, Sakae WATANABE, Yuji MATSUSHIRO, Masahar ...
    1991 Volume 32 Issue 6 Pages 657-663
    Published: June 25, 1991
    Released on J-STAGE: July 09, 2009
    JOURNAL FREE ACCESS
    Hepatocellular carcinoma (HCC) is usually hyperintense on T2-weighted magnetic resonance imaging (MRI), and there have been no reports on HCC presenting a hypointense signal on T2-weighted MR images. We, however, encountered 2 cases of HCC with hypointense signals on T2-weighted MR images. Their main tumors measured 29×22mm in Case 1 and 30×25mm in Case 2. These tumor showed high signal intensity on inversion recovery (IR) images, and low signal intensity on T2-weighted spin echo (SE) images in both cases. Aithough the tumors were histologically identified as typical HCC of Edmondson's grade I-II, and they showed no specific features characteristic of such signal hypointensity when stained with HE, the cytoplasm of these tumors was found to have black pigments of copper when stained with rubeanic acid. These rusults suggested that hypointense signals of HCC on T2-weighted images were closely related to copper deposition.
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  • Hiroshi TOKUYAMA, Kenichi AIZAWA, Takayuki YONEZAWA, Yasuhiko MATSUZON ...
    1991 Volume 32 Issue 6 Pages 664-669
    Published: June 25, 1991
    Released on J-STAGE: July 09, 2009
    JOURNAL FREE ACCESS
    A 69-year-old man admitted because of a chief complaint of disturbed consciousness. Slightly decreased liver function, elevated ammonia blood levels, retention of ICG in serum at 15 minutes, and slight atrophy of the liver on CT scans were suggestive of the diagnosis of hepatic encephalopathy. Left renal venography demonstrated splenorenal-gastrorenal shunt. The histologic examination of the liver showed hemosiderosis. However, there was no liver cirrhosis or fibrosis that caused an appreciable portal hypertension. A shunt occlusion test using a baloon catheter was performed and ammonia levels and portal pressure values were measured before and after occlusion. The ammonia tolerance test following occlusion of the shunt showed no increase in ammonia level and a slight elevation of portal pressure. These results revealed that the portal blood entered the systemic circulation through the shunt. Portal-systemic encephalopathy without associated liver cirrhosis and fibrosis that cause prominent portal hypertension, as in this case, is rare. In this case, there was a past history of abdominal surgery and adhesion formed postoperatively appeared to be involved in the development of the shunt.
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  • Hitoyoshi OHTA, Minoru ONO, Motoyuki OHHIRA, Chihiro SEKIYA, Masayoshi ...
    1991 Volume 32 Issue 6 Pages 670-671
    Published: June 25, 1991
    Released on J-STAGE: July 09, 2009
    JOURNAL FREE ACCESS
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  • Norio UENO, Mineo ENOMOTO, Ken KIMURA
    1991 Volume 32 Issue 6 Pages 672-673
    Published: June 25, 1991
    Released on J-STAGE: July 09, 2009
    JOURNAL FREE ACCESS
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  • [in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    1991 Volume 32 Issue 6 Pages 674
    Published: June 25, 1991
    Released on J-STAGE: July 09, 2009
    JOURNAL FREE ACCESS
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