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Volume 55 (2001)
- Issue 6 Pages 331-
- Issue 5 Pages 283-
- Issue 4 Pages 243-
- Issue 3 Pages 189-
- Issue 2 Pages 123-
- Issue 1 Pages 1-
Volume 55, Issue 2
Displaying 1-6 of 6 articles from this issue
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Original Articles
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Teruyuki Hirakawa, Jun-ichi Ishimaru, Yujiro Handa, Ken-ichi Kurita, N ...2001 Volume 55 Issue 2 Pages 123-136
Published: April 25, 2001
Released on J-STAGE: August 03, 2007
JOURNAL FREE ACCESSThe masseter muscle of the Australian Merino sheep consists of three layers, superficial (MS), middle (MM) and deep (MD). We studied the EMG activities of jaw elevator muscles including the three layers of the masseter muscle during mastication. The EMGs were recorded with a bipolar electrodes of stainless wire which was coated with enamel, and from ten muscles as follows, temporal muscle (T), MS, MM, MD and medial pterygoid muscle superficial fiber (MP) on both sides. Masticatory movements were induced by feeding. Three types of feed were given ; grasses as a fibrous type, oats as an elastic type and sheep nuts as a solid type. In T, MD and MM, the discharge volumes of the EMGs on the working side were larger than those on the balancing side, but in MS and MP, the durations on the working side were smaller than those on the balancing side. The starting times of activities of these muscles in mastication were in sequence, MD, MM and MS on the working side, MD, MS and MM on the balancing side. The starting time of MD on the balancing side in mastication arose earliest. Therefore, the activity patterns of these muscles reveal which is the working side and which is not. It is recognized that the activities of the three layers of masseter muscle differed from one another in mastication.View full abstractDownload PDF (874K) -
Tsuneo Yamamoto2001 Volume 55 Issue 2 Pages 137-145
Published: April 25, 2001
Released on J-STAGE: August 03, 2007
JOURNAL FREE ACCESSThe functional implication of complicated anatomical and neurochemical events of the spinal cord has been progressibly clarified. Although there are many evidences implicated as the primary sensory transmitter of acute pain in spinal cord, the reasons why the accelerates is not clear. We attempted to clarify the changes in local spinal cord glucose utilization (LSCGU), and protein kinase C (PKC) activation / translocation by MO (mustard oil) injection in relation to pain behaviour.
Fourteen male Wistar rats were subjected to MO testing. The flinching of the injured paw was counted every 5min for the duration of 60min period. For the determination of LSCGU, 14C-2-deoxyglucose (14C-2DG) method was performed in the spinal cord at the same level as the 2nd and 5th cervical vertebrae for 60 min after MO injection. Simultaneously, using same frozen tissue, the binding sites of 3H-phorbol-dibutylate (PDBu) for PKC activity were determined by means of quantitative in vitro autoradiography.
Following results were obtained :
1. The number of flinching after MO injection increased significantly in the MO group more than in the control group during 15min to 60min period.
2. 14C-2DG was increased significantly at laminae I-II and V-VI in the ipsilateral spinal cord at the same level as the 5th cervical vertebrae in the MO group than in the contralateral spinal cord in the MO group and bilateral spinal cord in the control group.
3. Bindings for 3H-PDBu at laminae I-II in the spinal cord were the densest, and binding at laminae I-II and V-VI in the ipsilateral spinal cord at the same level as the 5th cervical vertebrae in MO group than in the contralateral spinal cord in MO group and bilateral spinal cord in control group.
The present study revealed the hyperalgesia in inflammatory pain by mustard Oil injection caused the spinal sensitization by the increase in LSCGU and the activation of PKC.View full abstractDownload PDF (992K) -
Masafumi Yoshinari2001 Volume 55 Issue 2 Pages 146-154
Published: April 25, 2001
Released on J-STAGE: August 03, 2007
JOURNAL FREE ACCESSThe mechanism of the hyperalgesia may be mediated by the modulation of the transmission to the WDR neurone from C-fiber in posterior horn of spinal cord. The glutamate in celebrospiral fluid (CSF) are possibly concerned in pain modulation. However, role of these synaptic regulation exerted by concurrent glutamate release and pain response is not fully understood. The purpose of the present study was to investigate whether the inhalation of nitrous oxide modulated pain-related response in relation to glutamate in (CSF) following formalin induced nociception in rats.
Three days after intrathecal implantation of PE-10 catheter along with loop-type microdialysis probe in male wister rats, 50 μl of 5 % formalin was subcutaneously injected into left hind paw. Thereafter, simultaneous determinations of microdialysis at 10 min. -intervals for glutamate by HPLC-THI and observation of flinches were performed for 60 min after inhalation of four type nitrous oxide concentration (control group : 70 % nitrogen with 30 % oxygen, 30 % N2O group : 30 % nitrous oxide with 70 % oxygen, 50 % N2O group : 50 % nitrous oxide with 50 % oxygen, 70 % N2O group : 70 % nitrous oxide with 30 %).
Biphasic increases of flinches after injection of formalin were observed ; phase 1 (13 flinches/min. at 10 min.) and phase 2 (11 flinches/min. at 40 min.). 30 % and 50 % nitrous oxide attenuated flinches during phase 2 period. 70 % nitrous oxide attenuated flinches during both phase 1 and phase 2 periods. CSF glutamate in control group was transiently increased by 145±8 % and 126±7 % during 10 min and 20 min, respectively. This increase of glutamate was supressed by 30 %, 50 % and 70 % nitrous oxide.
The present results clearly demonstrated that nitrous oxide inhibits the both phases of flinchings associated with supressed glutamate release. The analsic effects of nitrous oxide are dose dependence, and these effects may regulate neurotransmitter release evoked by C fiber activation.View full abstractDownload PDF (819K) -
Mariko Naito, Toshiko Tanaka2001 Volume 55 Issue 2 Pages 155-164
Published: April 25, 2001
Released on J-STAGE: August 03, 2007
JOURNAL FREE ACCESSA combination of 5-fluorouracil (5-FU) and cisplatin (cis-Diamminedichloroplatinum (II) ; DDP) was administered to the human pharyngeal carcinoma KB cell line in vitro in order to examine the effects of 5-FU pretreatment on DDP cytotoxicity and binding of platinum (Pt) to DNA (Pt-DNA) or its removal from DNA. DDP cytotoxicity was determined by cell survival in the presence (0.5, 1.0 μg/ml) or absence of 5-FU pretreatment. Cultures were exposed to 5-FU for 24 h in Eagle's minimum essential medium (MEM) with 10 % fetal bovine serum, and treated with DDP for 2 h after a 24 h drug-free interval in arginine-deficient MEM (ADM) containing 2.5 % dialyzed fetal bovine serum (DFBS), and then incubated for the required time interval in ADM with DFBS. After 0, 6, 12, 24 h postincubation, DNA was isolated from the cells, and Pt-DNA was measured by atomic absorption spectrophotometry.
By comparing 50 % inhibitory concentration in survival, the pretreatment with 0.5 and 1.0 μg 5-FU/ml increased DDP cytotoxicity 1.4- and 1.7-fold in KB cells, respectively.
The amount of Pt-DNA in KB cells, which was measured immediately after treatment with DDP, increased with doses up to 2.0 μg/ml of 5-FU, and the cells treated with 10.0 μg/ml of DDP had approximately 1.7-fold higher Pt-DNA than the cells treated with 5.0 μg/ml of DDP at each 5-FU dose tested.
In the time-course study, the cells exposed to 0.5 μg 5-FU/ml lost 19.5 and 42.7 % of their total Pt-DNA at 12 and 24 h after 5.0 μg DDP/ml treatment, respectively, compared with 46.4 and 47.6 % for the cells without 5-FU exposure. The cells exposed to 1.0 μg 5-FU/ml lost 24.4 and 31.7 % of their total Pt-DNA at the same respective times. The cells exposed to 0.5 μg 5-FU/ml also lost 16.4 and 31.1 % of their total Pt-DNA at 12 and 24 h after 10.0 μg DDP/ml treatment in comparison with 34.7 and 57.9 % for the cells without 5-FU exposure, and the cells exposed to 1.0 μg 5-FU/ml did 16.4 and 25.6 % of their total Pt-DNA at those times.
These results indicate that 5-FU pretreatment is associated with both increasing effects on Pt binding to DNA and retarding effects on the removal of Pt-DNA in a KB cell line, thereby potentiating the cytotoxicity of DDP.View full abstractDownload PDF (681K) -
Akemi Uchida2001 Volume 55 Issue 2 Pages 165-178
Published: April 25, 2001
Released on J-STAGE: August 03, 2007
JOURNAL FREE ACCESSThe purpose of this study is to clarify the relationship between the findings of contrast-enhanced CT scans and the histopathological findings of regional lymph nodes obtained from a complete extirpation of oral squamous cell carcinoma. Twenty-seven pairs of contrast-enhanced CT scans and histopathological specimens of 27 oral squamous cell carcinoma used in this study were obtained at Kyushu Dental College Hospital between 1987 and 1999. The primary site of the 27 tumor consists of the tongue (13 cases), the mandibular gingiva (6 cases), the maxillary gingiva (4 cases), the floor of the oral cavity (3 cases), and the buccal mucosa (1 case).
The results obtained were as follows :
1. Of the 27 cases, 11 (41 %) were proven to be positive lymph nodes metastatic cases histopathologically. Metastatic lymph nodes were predominantly detected in both the submandibular regions and the superior internal jugular chains (95 %).
2. The length and the ratio of the short and long axes of the metastatic lymph nodes were larger than those of the non-metastatic lymph nodes.
3. Many of the metastatic lymph nodes revealed a low density in their central area and were also accompanied by a thin ring enhancement. Non-metastatic lymph nodes, affected by inflammation, often revealed a heterogeneous density.
4. The ring enhancement was predominantly seen in the metastatic lymph nodes (45 %), which were filled with a well-differentiated tumor cell. In the metastatic lymph nodes, which were filled with moderately-differentiated tumor cells, were seen heterogeneous density, but a ring enhancement was not seen.
5. In the metastatic lymph nodes, it was difficult to decide the metastatis by contrast CT scan, if the tumor extension degree was not predominant.
6. The ring enhancement was caused by the dilatation of the capsular vessels, but not by the extra capsular spread of the tumor cells.View full abstractDownload PDF (1383K)
Case Report
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Mika Toyoda, Hiroshi Baba, Hisato Ishii, Toshikazu Takao, Tetsuo Okayo ...2001 Volume 55 Issue 2 Pages 179-186
Published: April 25, 2001
Released on J-STAGE: August 03, 2007
JOURNAL FREE ACCESSWe encountered a very difficult and highly suggestive edentulous case not experienced to date, the existing upper denture rotated horizontally from the median vertical axis of face to the part of angulus oris on a large scale when occluding slightly at the right molar region, and the lower denture depressed greatly to be upset when pressing downwards by a finger on the left lower molar region because of remarkable absorption of the underlyning bearing bone tissues.
In the process of newly constructing denturers, the suggetions obtained were as follows :
1) The median line of the upper denture should be decided through the median palatine suture and the incisal papilla even if their positions were dislocated greatly, without referring the median line on the cast judged by appearance.
2) In the restoration for complicated edentulous cases, it is essential to establish occlusion of the dentures regarding the most stabilized area when pressing downwards by fingers the occlusal surface of the existing lower denture or the lower occlusion rim as important.
3) Not only molars of the normal ridge side, but also ones of the side where are not able to secure support from the basal seat of the dentures should provide a slight occlusal contact for constructing the patient's adaptive denture.
4) Esthetic contentment also might be an efficient aid to the patients who are endeavoring after functional contentment, even if they are the aged and males.
As described above, we felt strongly through this specific case the necessity of muscle trimming and careful observation of casts, and of confirming the key zone for unilateral balanced occlusion, although they are always important on the construction of any complete dentures.View full abstractDownload PDF (1570K)
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