Thymosinβ4 (TB4), a 43 amino acid (a.a.) peptide, is regarded as the main globular-actin (G-actin) sequestering peptide which binds to G-actin and prevents polymerization into actin filament. Recent studies have revealed that TB4 is a multifunctional peptide involved in cell migration, angiogenesis, wound healing, inflammation, morphogenesis and tumor metastasis. However, there are few reports associated with dental research, except for our previous reports suggesting the important role of TB4 in tooth germ development. In this study, we investigated the effects of TB4 on wound healing after tooth extraction, using a 20 a.a. synthetic peptide which includes the nine a.a. G-actin binding motif and the seven a.a essential site for angiogenic activity. After extraction of a rat mandibular first molar tooth, the synthetic peptide was injected intraperitoneously into each rat at the time of extraction and every day thereafter for four days. Controls for the treatment received identical amounts of phosphate buffered saline in the same manner. Histological sections were processed from decalcified mandibles of Day2, Day3 and Day4 cases. Histochemical and immunohistochemical stainings were also performed as needed. Total RNAs from granulation tissues in the sockets were isolated, and semi-quantitative RT-PCR for matrix metalloproteinase-2 (
MMP-2) and vascular endothelial growth factor (
VEFG) genes was performed.
MMP-2 was only amplifed in TB4-treated samples. Though
VEGF was detected in both TB4-treated and control samples, the expression level in TB4-treated cases was lower than with controls. Histological examination showed that the synthetic peptide accelerates the process of wound healing after tooth extraction such as absorption of blood clots, formation of granulation tissue, reepithelialization and new bone formation. These results demonstrate the usefulness of TB4 in dental clinical application.
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