In the central nervous system of various mammals, the presence and the ultrastructures of nucleolus-like inclusion bodies (inclusion bodies) have been illustrated of electron microscopic studies. In our previous studies, paraffin sections of ddY mouse brains stained according to the Holmes modified method clearly demonstrated inclusion bodies in the locus coeruleus under a light microscope. The present study investigated whether inclusion bodies in 11 strains of inbred mice were observable in the same pattern as ddY mice. Locations of inclusion bodies in whole ddY mouse brains were established. All ddY male and inbred strain mice were perfused under ether anesthesia via the left ventricle with a solution of 2% paraf ormaldehyde and 2.5% glutaraldehyde, and whole brains were then excised to prepare paraffin sections. Frontal serial sections of mouse brains were stained using the Holmes modified method. Detailed observation was made under a lightmicroscope. Inclusion bodies identified in most nerve cells in the locus coeruleus of ddY male mice (n=20) were counted, revealing a mean of 1552±1 36 bodies unilatelally without any differences between both sides. Inclusion bodies in the locus coeruleus of the 11 inbred strains were also counted, and most neurons of the locus coeruleus displayed one or two inclusion bodies. This result basically resembled that of ddY mice. Inclusion bodies in whole brains of ddY mice were also found in the amygdaloid, medial septal, and accumbens nuclei, the preoptic region, and the hypothalamus, the solitary tract nucleus, the substantia intermedia lateralis of the spinal cord, and the locus coeruleus (A6), the raphe nuclei (B1-9), and the organum vasculosum laminae terminalis, the organum subfornicale, the area postrema.
In summary, inclusion bodies were supposed as the construction which principally appeared at the neural cytoplasm in the limbic, autonomic, monoamine neuron systems, and the circumventricular organ.

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Concentrations of 21 elements in the kidney and liver of 71 health Japanese males were determined by instrumental neutron activation analysis. Elemental concentrations values were analyzed on the dry and wet weight basis. The following results were obtained: 1) A large number of elements were detected in both organs. Many of our data did not confirm the previously reported several respects. Distribution of the values in the present study was not widely scattered because the organs were selected from healthy individuals and the collection sites of the tissues were consistent. 2) Cadmium values of the Japanese were higher than those previously reported, indicating that cadmium is still accumulating in Japanese. 3) Changes of concentrations of the adulterant elements, such as As, Cd, Cs, La, Mo, Sb and Sc, might depend on the effects of external environmental factors. The distributions of these elements showed log-normal. In contrast, concentrations of essential elements, such as Br, Ca, Cl, Fe, K, Mn, Na, Rb, Se and Zn, were stably constant. Concentrations of these elements showed a normal distribution. Thus, it is suggested that external environmental factors did not affect concentrations of these elements. 4) Data in the liver suggested significant correlations between age and amount of elements more frequently than those in the kidney.

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