During the past fourteen years, there have been 225 cases of tuberculous meningitis in the medical service and 201 cases in the pediatric service of the Shitaya Hospitallin Tokyo.
In my experiment I used Tubercle bacillus and the so-called non-pathogenic acid-fast bacilli, and studied the essential cause for the variation of these bacilli from rod shaped bacilli to spherical or granular form in the fresh egg containing culture media. I have verified that the chief factors that cause the variation of acid fast bacilli are the protiens contained in the white of fresh eggs, that is, the thermolabile euglobulin, psudoglobulin; and albumin, which has not been subjected to heat; or heated below 60°C-70°C.