In the presence of various aminoacids as nitrogen source in Sauton culture, the cell growth rate of tubercle bacillus (avian Jucho strain) and its aminoacids utilization and their biosynthesis were investigated by paper chromatography.
1. In these experiments, no difference between streptomycin resistant strain (R-strain) and its sensitive one (S-strain), were found except in asparagine Sauton culture. In asparagine Sauton culture, S-strain exceeds R-strain in growth rate, and it may be said that the asparaginase activity in R-strain is a little higher than in S-strain.
2. The tubercle bacillus markedly utilizes L-asparagine, L-alanine, L-histidine, and L-arginine, and moderately L-aspartic acid, D-alanine, L- and D-glutamic acid, and glycine. DL-serine and DL-phenylalanine were little effective as its nitrogen source. L-lysine and DL-methionine were not utilized at all.
3. Concerning streoisomer of these aminoacids, L-form was more effective than D-form.
4. The aminoacids biosynthesis in their culture filtrates such as aspartic acid, glutamic acid, alanine, serine, and valine was observed by paperchromatography.
5. The pH of their culture filtrate inclines alkaline with their culture ages. When L-arginine and L-histidine were used as nitrogen source, the pH of their culture filtrate was lower then in other cases.
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