巻号一覧
29 巻, 4 号
選択された号の論文の7件中1～7を表示しています
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太田 哲司, 佐藤 昭雄1954 年 29 巻 4 号 p. 119-123
発行日: 1954/04/15
公開日: 2011/05/24
ジャーナル フリーOBSERVATIONS on the blood monocytes at the “Schub” in the pulmonary tuberculosis patients: The total counts of monocytes increased and then the ratio of monocytes and lymphocytes decreased. The nucleus form shifted to the left. The monocytes with lower and higher basophility increased and modera tely basophilic monocytes showed no remarkable change. The vacuoles and granules in cytoplasm increased generally. The square of cytoplasm increased remarkably in every case and that of nucleus too increased slightly. At the “Schub”, regeneration and degeneration of blood monocytes increased.抄録全体を表示PDF形式でダウンロード (1108K) -
第6報VoleTuberculinに就いて室橋 豊穂, 伊東 恒夫1954 年 29 巻 4 号 p. 124-127
発行日: 1954/04/15
公開日: 2011/05/24
ジャーナル フリーVOLE bacillus (murin type of M. tuberculosis) strain D15 was cultured on Sauton's synthetic medium. After each of eight, ten and twelve weeks cultivation, the culture filtrate was concentrated to 1/10 of the original volume by evaporation. The concentrated filtrate was used as the original solution of crude vole tuberculin. Ortho-amino-azo (O-A-Azo) vole tuberculin was also prepared from a part of the original solution following H. Okamoto and S. Koshimura's method. The crude vole tuberculin and the O-A-Azo vole tuberculin were compared with the respective preparations of the culture filtrate of human type M. tuberculosis, Aoyama, B, i. e. the standard old tuberculin and the O-A-Azo old tuberculin.
There was no significant difference among the vole tuberculins prepared from three different culture ages. Their potency was found equivalent each other both on human and on animal tests, all being nearly half of the potency of old tuberculin.
Lower potency of vole tuberculin may due to the smaller amount of active principle of tuberculin produced in Sauton's medium on which the growth of vole bacillus was distinctly dysgonic. It was interesting to note that the sensitization of guinea pigs with heat-killed vole bacillus suspended in paraffin il was stronger than that of Aoyama B.抄録全体を表示PDF形式でダウンロード (890K) -
小倉 克彦, 今津 史郎, 加藤 允彦, 山村 碓一1954 年 29 巻 4 号 p. 128-133
発行日: 1954/04/15
公開日: 2011/05/24
ジャーナル フリーESTERASE activities of various acid-fast bacilli and their extracts were measured by Rona and Lasnitzki's method.
1. The esterase activity of avian tubercle bacilli was heat-stable irrespective of cultural conditions.
2. There were two different types of esterases in acid-fast bacilli, heat-stable esterase (Esterase II) of avian type and heat-labile esterase (Esterase I) of thymotee type.
3. Esterase II was further subdivided into two different esterases, one being the butyl ester hydrolyzing esterase and the other being acetyl ester hydrolyzing esterase. The former was the most heat-stable (stable against the heating at 100°C for 30 minutes) and was inhibited markedly by oleio acid, iodine and atoxyl. The latter was inactivated by heating at 100°C for 20 minutes (stable against the heating at 80°C for 10 minutes) and fairly resistant against the influence of inhibitors.
Subdivision of Esterase I (heat-labile) was not practicable.
4. Purification by adsorption on silicious earth, fractionation with ammonium sulfate and acetone increased the butyl ester hydrolyzing activity of Esterase II about 15 times. The purified esterase was also heat-stable and had the characteristics of protein or polypeptide. By paper chromatography, 13 amino acids were shown in the acid hydrolysate of the purified esterase. The esterase activity was inhibited markedly by iodine, oleic acid and eserine.抄録全体を表示PDF形式でダウンロード (1192K) -
吉田 清一1954 年 29 巻 4 号 p. 134-137
発行日: 1954/04/15
公開日: 2011/05/24
ジャーナル フリー1. Ten antisera of the rabbits inoculated with human tubercle bacilli, which showed hemagglutinin titers ranging from 1: 32 to 1: 512, were tested repeatedly by hernagglutination reaction after the absorption test using tubercle bacilli as absorbant.
The fall in hemagglutination titer was generally confirmed following the absorption test by tubercle bacilli.
2. On the other hand, no significant change in the titer was observed in the same antisera following the absorption test by staphylococci.
Thus, it was strongly suggested that the hemagglutinin was closely connected with tubercle bacilli.3. In addition, some examinations were performed to clear the relationship between the value of γ-globulin fraction in serum and the hemagglutinin titer. It was found that a part of γ-globulin & Faction, not the whole γ-globulin, was closely related to the hemagglutinin itself.
It is supposed that there arc specific antibodies and “nonspecific γ-globulin” in the I-globulin fractions increased in the serum of the rabbit inoculated with tubercle bacilli.抄録全体を表示PDF形式でダウンロード (1108K) -
伊藤 義昭, 吉田 昇1954 年 29 巻 4 号 p. 138-142
発行日: 1954/04/15
公開日: 2011/05/24
ジャーナル フリーA bacteriologic study was made on 113 specimens of surgically removed lesions from 52 pulmonary tuberculosis patients.
These lesions were aseptically incised, and the contents were transferred directly, without NaOH treatment, on Ogawa's egg medium for the cultivation of M. tuberculosis. Five specimens of caseous materials from which the detection of M. tuberculosis.by cultivation might not be successful were also inoculated into guinea pigs. With some samples, cultivation of bacteria other than M. tuberculosis.and of fungi were also attempted in order to detect probable mixed infection. Smears were stained, usually, by the combined staining method of Ziehl and Heidenhain (Uyeda), and the number of acid-fast forms and that of easily-stainable forms (Uyeda) were determined.
Results were summarized as follows:
1. The number of colonies of M. tuberculosis.does not always coincide with the number of acid-fast forms, but agrees approximately with the number of easily stainable forms. We can well understand these findings if we take into consideration the opinion of Uyeda that the easily-stainable form must be the viable form of M. tuberculosis. In some particular cases, however, the easily-stainable forms were demonstrated but the finding of the cultivation was negative.
2. Though the smears were positive for acid-fast forms, the cultures were often negative even with the materials from the cavities or the liquefied lesions, especially small circumscribed lesions of the cases which had been treated with artificial pneumothorax.
3. The clear-cut difference in the morphology and the viability of tubercle bacilli from the patients treated and not treated with chemotherapeutics was not recognized.
4. Pulmonary tuberculous lesions are, in almost all cases, free from mixed infection.5. The previous treatment of pathogenic materials with NaOH for the cultivation of M. tuberculosis.remarkably decreased the number of colonies. Therefore, materials which were aseptically removed, for example, surgically resected pulmonary lesions, should be cultured without the preliminary treatment of NaOH.抄録全体を表示PDF形式でダウンロード (2698K) -
その1結核アレルギーに関する実験的研究山村 雄一, 矢坂 茂, 山口 正民, 遠藤 一男, 岩倉 弘之, 中村 滋, 小川 弥栄1954 年 29 巻 4 号 p. 143-146
発行日: 1954/04/15
公開日: 2011/05/24
ジャーナル フリーAN experimental method for making the tuberculous cavity in the rabbit's lung was devised. The method was as follws:
1. In order to develop the sensitivity to tuberculin, 1 ml of the following mixture was injected subcutaneously into the femoral portion of rabbits weighing approximately 2 kg repeating the injection for 4-5 times at the intervals of 5-7 days.抄録全体を表示PDF形式でダウンロード (2098K) -
吉田 敏郎1954 年 29 巻 4 号 p. 147-150
発行日: 1954/04/15
公開日: 2011/05/24
ジャーナル フリーP32-labeled tubercle bacilli (Aoyama B strain) were obtained from the cultures grown on the Kirchner's protein-free medium containing Na2HP32O4. The labeled bacilli were injected into vena auricularis or vela mesenterica of norni: 1 rabbits and rabbits previously inoculated with M. tuberculosis var. hominis for the observation of the distribution of P" in the tissues of various organs 30 min., 24 hrs., and 96 hrs. respectively after injection. The larger amounts of the labeled bacilli were demonstrated in the lung when the injection was performed into vena auricularis, and in the liver when injected into vena mesenterica, and there was no difference between normal and tuberculous rabbits. On the other hand, fine crystals of ammonium phospho-niolybdate containing P32 which had been injected in the same manner, were demonstrated in the liver, lung and kidney in an almost equal amount regardless to the route of injection, and also there was no difference between normal and tuberculous rabbits. Furthermore, the P32-labeled tubercle bacilli were distributed mainly in the lung of both normal rabbits and rabbits with strongly sensitized lungs and the same results were obtained in the case using a small amount of the labeled bacilli as well as the large amount. Consequently, we assumed that the distribution of the tubercle bacilli and ammonium phospho-molybdate in the organs of animals depends upon the physical and mechanical conditions such as the size or surface activity of the injected material. The “localization of antigen by the antigen-antibody reaction” which has been believed since Roessle, was not remarkable in the tuberculous allergy reaction.
Furthermore, in order to know the ratio of nonlabeled phosphate to P32-phosphate added in the medium for the cultivation of the best radio-active tubercle bacilli, the tubercle bacilli (avian type) were cultured for 7 days on kirchner's protein-free media which contained varying amounts of non-labeled phosphate and 50μc P32-phosphate. The radio-activity of the cells collected was compared. Consequently, the most radioactive P32-labeled bacilli were obtained from the medium in which 1/16 of the usual amount of non-labeled phosphate was used. The bacterial yield from this medium was the same as usual.抄録全体を表示PDF形式でダウンロード (925K)
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