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Volume 41, Issue 9
Displaying 1-4 of 4 articles from this issue
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Michio TSUKAMURA, Sumio TSUKAMURA, Shoji MIZUNO, Haruo TOYAMA1966 Volume 41 Issue 9 Pages 395-399
Published: 1966
Released on J-STAGE: May 24, 2011
JOURNAL FREE ACCESSOne hundred characters have been considered to be useful for classification and identification of mycobacteria. These characters showed either positive or negative reactions in t est mycobacteria, which had been maintained in this laboratory, except for four characters giving positive reaction in all strains, growth at 28°C and at 37°C, utilization of glutamate as nitrogen source, and utilization of glycerol as carbon source. These characters are as listed below.View full abstractDownload PDF (1147K) -
Michio TSUKAMURA, Sumio TSUKAMURA, Shoji MIZUNO, Haruo TOYAMA1966 Volume 41 Issue 9 Pages 401-406
Published: 1966
Released on J-STAGE: May 24, 2011
JOURNAL FREE ACCESSCharacters of slowly growing scotochromogenic mycobacteria isolated from patient materials in this country were studied. The results are shown in tables. These scotochromogens were distinguished from named species of mycobacteria at least in more than four characters and were considered to be a species. These mycobacteria were named Mycobacterium aquae.
The name of M. aquae had been used by Galli-Valerio and Bernard in 1927 for a scotochromogen, and Bönicke proposed this name for scotochromogens, based on the similarity of amidase pattern. Basis of creating a new species was provided in our study by adding a number of characters giving “positive matches”. The outline of the new species and the differentiation from other species were clarified.
The term scotochromoge n used here indicates, as described in the preceding paper, an organism developing markedly yellow or orange colonies on Sauton agar from its initial growth.View full abstractDownload PDF (1061K) -
Toyoho MUROHASHI, Konosuke YOSHIDA1966 Volume 41 Issue 9 Pages 407-410
Published: 1966
Released on J-STAGE: May 24, 2011
JOURNAL FREE ACCESSDifferentiation of Nocardia from Mycobacteria is generally not difficult according to the cultural properties. Sometimes, however, it is not easy to differentiate them, especially in the microscopic examination of sputum specimens, owing to the acidfast stain of the fragments of some of the Nocardia.
As report e d previously, analytical studies carried out on the decolorization procedures of the acidfast staining led us to the finding that within 20 minutes rinsing in a decolorizer composed of O.1-0.5% HNO3 or HC1 in 70% (v/v) Ethanol resulted in the complete loss of the stain of saprophytic Mycobacteria. In the present studies, two kinds of acid-ethanol, namely, 0.1% HNO3-70% Ethanol and 4% HNO3-Ethanol, were used for the decolorization.
The results revealed that the former was very effective in decolorizing Nocardia almost completely by less than 30 seconds' rinsing, by which time Mycobacteria retained nearly 50%of stain. On the contrary, by the use of the latter, the commonly used decolorizer, such a marked difference was not observed between Nocardia and Mycobacteria.
Based on the results obtained, a very simple method was devised to differentiate Nocardia from Mycobacteria by the acidfast stain of smears employing 0.1% HNO3-70% Ethanol as a decolorizer for 10 to 30 seconds.View full abstractDownload PDF (2342K) -
1966 Volume 41 Issue 9 Pages 411-446
Published: 1966
Released on J-STAGE: May 24, 2011
JOURNAL FREE ACCESSDownload PDF (7247K)
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