Although previous studies on the incidence of unclassified mycobacteria in Japan revealed the rate of 0.2 to 3.5% in healthy persons, 0.5 to 1.3% in patients in tuberculosis sanatorium and 0.6 to 12.5% in factory workers, the incidence of unclassified mycobacteria in patients with non-tuberculous respiratory diseases has not yet been reported in Japan. The purpose of this paper is to isolate unclassified mycobacteria am o ng patients with nontuberculous respiratory diseases and to differentiate unclassified mycobacteria from mycobacterial strains cultured at the laboratory. Unclassified mycobac t eria was isolated and differentiated from the materials in each of the following three groups: The first group consists of sputa taken from 142 cases with nontuberculous respiratory diseases from February 1963 to February 1965, the second group consists of mycobacterial strains which were obtained by mycobacterial culture of sputa and gastric lavage at the laboratory from September 1964 to February 1965, the third group, mycobacterial strains, which showed unusual colony formation and/or resistance to antituberculous drugs(SM, PAS, INH) though previously untreated, from February 1963 to August 1964. As the results of this study, 20 strains of unclassified mycobacteria we r e obtained, the total rate of isolation of unclassified mycobacteria was 1.1%, and it was 4.9% in the first group and 0.4% in the second groud Therefore a high incidence was revealed in the patients with non-tuberculous respiratory diseases. In the second group, 3 s t r ains (5.3%) of unclassified mycobacteria were differentiated from 56 mycobacterial strains. It suggests that the differentiation between unclassified mycobacteria and Mycobacterium tuberculosis is necessary as a routine procedure in the laboratory. The diagnosis of the patients, at the isolation, was as follows: 13 ca s es of pulmonary tuberculosis, 2 cases of pulmonary emphysema, 1 case of chronic bronchitis, 1 case of pulmonary cancer, 1 case of sarcoidosis, 1 case of systemic lupus erythematosus and 1 case of Goodpasture's syndrome. No ca s es with non-tuberculous respiratory diseases fulfilled the diagnostic criteria of unclassified mycobacteriosis by Hibino. According to Runyon's classi f ication, 18 strains were Nonphotochromogens, 1 strain was Scotochromogens and another 1 strain was Rapid growers. The frequency of isolation of unclassified myc o bacteria was only once in 11 cases, twice in 4 cases, 3 times in 3 cases, 5 times in 1 case and 7 times in 1 case.
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