巻号一覧
52 巻, 1 号
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豊原 希一1977 年 52 巻 1 号 p. 1-6
発行日: 1977/01/15
公開日: 2011/05/24
ジャーナル フリーGrowth of tubercle bacilli in normal macrophages was inhibited by adding the immune lymphocytes or the culture supernatant in vitro. The inhibition was increased by adding PPD intoculture.
Culture supernatant of the immune lymphocytes increased acid-phosphatase activity of normalmacrophages, but the effect of adding PPD into culture was not significant to acid-phosphataseactivity of macrophages.抄録全体を表示PDF形式でダウンロード (853K) -
Abdel Kader, Abdel Aziz, M.A. Shawky, Abdel Hakim, M. Amr, I. Soultan1977 年 52 巻 1 号 p. 7-10
発行日: 1977/01/15
公開日: 2011/05/24
ジャーナル フリーIn active tuberculosis there is a significant decrease in plasma phospholipids in the 3 stages of tuberculosis, compared to normal, due to liver affection while there is a significant increase in plasma triglycerides in the 3 stages of active tuberculosis due to decrease in the serum lipase activity. Total serum lipids increased significantly in stage I of the active disease but without a significant difference shown in stage II & III of theactive disease. Total plasma cholesterol showed a significant decrease in stage III of the active disease without. a significant difference appearing in stage I & II.
Free plasma cholesterol showed a significant decrease in stage III of the disease without a significantdifference observed in stage I & II.
Cholesterol ester showed a significant increase in stage I of the active disease, to decrease significantly instage III without a significant change in stage II. Phospholipids, however, were significantly decreased duringthe different stages of the disease.
Serum lipoprotein pattern showed a highly significant increase in the non-mobile fraction in all stages oftuberculosis, most probably due to the increase of triglycerides. The β-lipoprotein fraction showed a highlysignificant decrease in stage III of the active disease, with insignificant changes in stage I & II.
The jlipoprotein fraction decreased significantly in stage I & II with insignificant change in stage III.
Serum lipase activity decreased significantly in active tuberculosis and significantly increased after treatment but not up to the level in normal persons.抄録全体を表示PDF形式でダウンロード (436K) -
常盤 寛, 武谷 健二1977 年 52 巻 1 号 p. 11-15
発行日: 1977/01/15
公開日: 2011/05/24
ジャーナル フリーMycobacteriocin produced by human type tubercle bacilli could be demonstrated easily on eggmedium containing 0.05% tween 80 by means of stab culture or streak plate methods, and elevenmycobacteriocin-types have been recognized on the basis of the inhibition pattern by using 9indicator strains of rapidly growing mycobacteria.
At present, types 1 (4.9%), 2 (13%), 4 (38.5%), 9 (2%) and 11 (14.8%) showed a stablemycobacteriocin pattern, but types 3 (7.8%) and 7 (6.7%) were apt to show type replacement totypes 2 and 4, respectively, and strains belonging to types 5, 6 and 8 were few, less than 1.5%to the total. In order to perform a reproducible mycobacteriocin typing, our previous typingscheme was revised to be consisted of five groups (A-E). Among 438 strains, mycobacteriocingroup C containing types 4 and 7 was 46.1% of strains tested, group B containing types 2, 3 and6 was 22.6%, group E containing type 11 was 15.1%, group A containing types 1 and 8 was5.5%, group D containing types 9 and 10 was 4.1%, and untypable strains were no more than 6.6% among all tested strains.
Human type tubercle bacilli classified as type 4, which consisted of more than 45% of strainstested, could be divided into three sub-types from sensitivity to M. gordonae 1324 and M. terrae 1450.
Mycobacteriocin producing strains belonging to type D: 9 showed an extremly wide antibacterialactivity to strains of more than 90% of rapidly growing mycobacteria (42 strains), to about 40% of slow growing mycobacteria (29 strains), and even to 10 strains of Staphylococcus aureus.抄録全体を表示PDF形式でダウンロード (4310K) -
束村 道雄1977 年 52 巻 1 号 p. 17-20
発行日: 1977/01/15
公開日: 2011/05/24
ジャーナル フリーThe conditions necessary for studies of the cross-resistance of tubercle bacilli (Mycobacterium tuberculosis) are discussed in the present paper.
1) Strains should be derived from single colonies. This is the most important, basic conditionto obtain clear-cut results. Use of mixtures of sensitive and resistant organisms or of resistantorganisms of different phenotypes may lead to misunderstanding of the nature of cross-resistance.
2) The degree of resistance (phenotype) should be estimated by a most sensitive method, forexample “actual count method” (Tsukamura, M.: Med. & Biol., 49: 87, 1958; Jap. J. Tuberc., 12: 46, 1964; Canetti, G. et al.: Bull. Int. Un. Tuberc., 37: 185, 1966). The method for estimatingthe phenotype may be changed according to the purpose of studies. In order to deal with a largedifference of the resistance, a non-quantitative method may be used, whereas, to deal with a littledifference, the “actual count method” must be used. The “actual count method” is a method in which the degree of resistance is estimated by the highest concentration of a drug in which 20 to 50 (if this is unavailable, 10 to 100) viable organisms are able to grow.
3) Sensitive organisms are defined as a population which is not exposed to any drug previouslyor a population which shows the same phenotype as the above population, and resistant organismsare defined as resistant mutants which retain heritably resistant properties. Unheritable resistantproperties are due to the phenomenon of “pseudoresistance”; the use of heavy inoculation makesvisible residual growth of sensitive organisms.
4) Cross-resistance studies should be done on different phenotypes. The number of resistantphenotypes of M. tuberculosis (H37Rv) remains usually a few.
5) From descriptive convenience, the cross-resistance was divided into the following categories: (1) complete cross-resistance (Resistant organisms selected by a drug A are resistant to drugs Aand B, and those selected by a drug B are also resistant to both drugs); (2) one-way crossresistance (Resistant organisms selected by a drug A are resistant to drugs A and B, but thoseselected by a drug B are resistant to only the drug B); (3) “major” cross-resistance (Resistantorganisms selected by a drug A are resistant to another drug B, and the increase of resistance toa drug B is more than ten-times when compared with the degree of resistance of the parentstrain); (4) “minor” cross-resistance (Resistant organisms selected by a drug A are resistant to adrug B, and the increase of resistance to the drug B is less than five-times when comparedwith the degree of resistance of the parent strain).抄録全体を表示PDF形式でダウンロード (636K) -
第3報実験的マウス結核症に対するTUM-NおよびINHの効果清水 辰典, 横山 道夫, 吉田 宇角, 大沼 廣明, 成田 昭彦, 奥山 富三1977 年 52 巻 1 号 p. 21-27
発行日: 1977/01/15
公開日: 2011/05/24
ジャーナル フリーThere have been several reports on the antituberculous activity of Tuberactinomycin-N (TUMN). The present report discusses the therapeutic effect of TUM-N on lung lesion of experimentalmouse tuberculosis.To DD strain mice weighing 20 grams, Ravenel strain of Mycobacterium bovis was intravenously inoculated and from one week after the challenge 0.1ml of 8mg/m/ INH solution and 20mg/m/ TUM-N solution was subcutaneously administered every day. Experiments were made for 5 weeks by killing 5 mice every week, respectively, for INH and TUM-N groups.
The homogenized spleens were cultured on 1% Ogawa's media for 8 weeks, and thereafterthe comparison was made among INH, TUM-N and the control groups by counting the bacterialcolonies. The growth of bacteria in the groups treated with INH and TUM-N was apparentlysmaller than that of the control group (Table 1).
The lung lesions were histopathologically examined after macroscopic observation. The INHand TUM-N groups showed much weaker tubercle formation, exudative inflammation and caseouspneumonia than the control group (Table 2 and Photo. 1-12).
The histopathological examination of the liver revealed weaker lesions in the treated groups (Table 3).
The antituberculous activity of TUM-N and INH was confirmed by the quantitative culturingof the spleen and the histopathological examination of the lung, but TUM-N had weaker antituberculous activity than INH. The present experiment indicated no adverse effect of TUM-N onthe kidney.抄録全体を表示PDF形式でダウンロード (5849K)
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