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Volume 55, Issue 9
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[in Japanese]1980 Volume 55 Issue 9 Pages 393-399
Published: September 15, 1980
Released on J-STAGE: May 24, 2011
JOURNAL FREE ACCESSDownload PDF (5929K) -
I. In Vitro Analysis of Suppression of BCG Cell Wall Induced Delayed Type HypersensitivityKazuyuki KATO1980 Volume 55 Issue 9 Pages 401-406
Published: September 15, 1980
Released on J-STAGE: May 24, 2011
JOURNAL FREE ACCESSPrevious injection of live BCG in mice produced a suppression of delayed hypersensitivity induced by oil-treated BCG cell walls (CW). This phenomenon was analyzed by the macrophage migration inhibition (MI) test in which peritoneal exudate cells (PEC) from live BCG-injected mice were mixed with PEC from BCG CW-immunized mice, and observed the former's suppression on the MI activity of the latter. The author examined the cell population involved in this suppression and found that the adherent cells of PEC did possess a suppressive effect which was retained even after treatment with either anti-mouse Ig serum or anti-brain associated θ antigen serum. This suggests that the suppressor cell may belong to macrophage compartments.Furthermore, whether or not the suppression of delayed-type hypersensitivity by the pretreatment with live BCG is antigen-specific was examined in mice immunized with heat-killed Listeria monocyto genes. As a result, the live BCG-pretreatment produced a significant suppression of footpad response induced with Listeria antigen, suggesting that this suppression is not-antigen-specific.View full abstractDownload PDF (764K) -
Michio TSUKAMURA, Shoji MIZUNO, Hiroshi MURATA1980 Volume 55 Issue 9 Pages 407-410
Published: September 15, 1980
Released on J-STAGE: May 24, 2011
JOURNAL FREE ACCESSMycobacterium nonchromogenicum Tsukamura (1965), M. terrae Wayne (1966), M. novum Tsuka mura (1966), and M. triviale Kubica et al. (1970) are slowly growing, nonphotochromogenic myco bacteria belonging to Runyon's Group III and are isolated from sputum specimens. These are closely related to each other. M. novum is considered as a synonym of M. terrae. It was reported that M. nonchromogenicum and M. terrae are differentiable from each other by nicotinamidase and pyrazinamidase activities and some other properties, but it is also certain that these are very closely related. The purpose of the present study is to search useful characters which are able to differentiate between these two organisms. The results of the present study have shown that the following characters are useful: nicotinamidase, pyrazinamidase, nitrate reduction, heat-stable acid phosphatase, growth at 42°C, and thin-layer chromatography after uptake of 35S-methionine.
M. nonchromogenicum usually grows at 42°C, shows positive heat-stable acid phosphatase activity, and shows a radioactive spot at Rf value 0.94 to 0.98 in thin-layer chromatography. In contrast to the above, M. terrae does not grow at 42°C, does not show heat-stable acid phosphatase activity, and does not show any spot at the Rf value 0.94 to 0.98 in thin-layer chromatography. Usefulness of the heat stable acid phosphatase activity for differentiating these two organisms was shown by Saito et al. This was confirmed by the present study. The other two useful characters, the growth at 42 and the presence or absence of the radioactive spot at the Rf value 0.94 to 0.98, were added in the present study. The substance which shows the spot at the Rf value 0.94 to 0.98 is extracted from bacterial cells by diethyl ether-ethanolView full abstractDownload PDF (543K) -
Eiko KONDO, Koomi KANAI1980 Volume 55 Issue 9 Pages 411-414
Published: September 15, 1980
Released on J-STAGE: May 24, 2011
JOURNAL FREE ACCESSExperimentally infected tuberculous mice were treated by intensive chemotherapy with three drug combinations (SM×INH×RFP, EB×INH×RFP or SM×INH×PZA) for 5 months so that viable counts from the spleen and lung were decreased down below the undetectable level.
After termination of chemotherapy, the mice of each group were divided into two groups, which subsequently received or did not receive intravenous administration of, β-1, 3 glucan, 0.5mg once a week, for 4 weeks and again 4 weeks after one month interval.
During this period and the succeeding 5 months, the mice were subjected to occasional sacrifice at random for cultivation of the two organs to detect the reincrease of latent tubercle bacilli therein. The results indicated that the regimens with SM×INEH×RFP or with EB×INH×RFP was highly efficient in eradicating infecting tubercle bacilli in mice, if not perfectly, and the use of β-1, 3 glucan as an immunoadjuvant was effective in preventing the reincrease of latent bacilli.View full abstractDownload PDF (441K) -
Kazuhiko KAMEDA, Nobu KUCHII, Fujiko HORII, Utako NAKAHARA, Junko OTSU ...1980 Volume 55 Issue 9 Pages 415-421
Published: September 15, 1980
Released on J-STAGE: May 24, 2011
JOURNAL FREE ACCESSThe follow up study of family contacts of tuberculosis patients have been continued for four years after the detection of index cases in order to know to what extent the family contacts examination was performed at the health centers in Osaka prefecture and how many cases requiring treatment were discovered among total household members.
This report is the over all results of four years, follow up. The survey on the fourth year was re peated in the same way as in the past three years.
Out of 815 patients newly registered at the 12 health centers of Osaka prefecture during the period from January to June, 1976, 357 were omitted from the registry by the end of October, 1978, and 1250 family members of the remaining 458 patients were subjected to study.
Out of 1230, 430 (34.4%) were examined by the contacts examination (Table 1). The atten dance rate of contacts examination showed marked difference according to health centers (Table 2).
One new case was found by the examination. The detection rate of contacts examination was 0.2%. Another two cases were found by symptomatic visit to clinic. Total number of new cases requiring treatment among family members during the fourth year after the discovery of index cases was 0.2% (Table 2). Among family members, 274 (21.9%) have not been examined at all during four years after the discovery of index cases and only 47 (3.8%) have been examined every year (Table2, 3, 4).
During the past four years, altogether 48 cases were found (Table 5). The detection rate was higher in the first and second years, especially among contacts of bacillary cases and it markedly fell down in the third and fourth years (Table 6, 7, Fig. 1).
It can be concluded that the contacts examination should be thoroughly carried out in the first two years after the discovery of index cases, especially for contacts of bacillary cases.View full abstractDownload PDF (841K) -
Izuo TSUYUGUCHI1980 Volume 55 Issue 9 Pages 423-427
Published: September 15, 1980
Released on J-STAGE: May 24, 2011
JOURNAL FREE ACCESSIt was found that there were two different subsets of thymus derived T lymphocytes when lympho cytes from patients with tuberculosis were stimulated in vitro with tuberculin purified protein derivative, PPD. The one was IgG Fc-receptor bearing T cells (TG) and the other was rosette forming T cells with autologous erythrocytes. Both of which are supposed to play an important role not only in the induction of tuberculin hypersensitivity but also in immunity to tuberculosis.
1) Increase in IgG Fc-receptor bearing T cells (TG) in PBL of tuberculous patients by the in vitro stimulation with PPD
A mixed rosette technique with sheep erythrocytes and chicken erythrocytes coated with heat aggregated human IgG was employed to identify human peripheral blood T lymphocytes bearing IgG Fc-receptor (TG). When peripheral blood lymphocytes (PBL) from patients with advanced, refrac tory tuberculosis were stimulated in vitro with PPD, increase in the number of TG cells was observed, whereas no such TG cells were developed in PBL from newly diagnosed tuberculous patients after the stimulation with PPD. TG cells isolated from the tuberculin skin test positive individual by the method of velocity sedimentation suppressed PPD-induced proliferative response of autologous PBL, as well as the pokeweed mitogen-induced IgG synthesis by B cells.
2) PPD-stimulated development in tuberculosis of T cells bearing receptors for autologous erythrocytes Tuberculous human lymphocytes activated in vitro with tuberculin PPD were examined for rosette formation with autologous erythrocytes. The autorosette forming cell (auto-RFC) levels were strongly enhanced when pleural fluid lymphocytes and, to a lesser extent, PBL of the patients with tuberculosis were stimulated in vitro with PPD, whereas no increase in auto-RFC was observed in PBL from tuberculin skin test negative healthy individuals. These auto-RFC developed were shown to rosette with sheep erythrocytes and, therefore, belong to a T cell subset. It was also shown that adherent cells were required for the development of auto-RFC by the stimulation with PPD. Deple tion of PPD-stimulated auto-RFC by the velocity sedimentation technique led to a significant de crease in PPD reactivity assessed by the proliferation or auto-RFC formation.led to a significant de crease in PPD reactivity assessed by the proliferation or auto-RFC formation.View full abstractDownload PDF (562K) -
On Aspect of Basic StudiesShiro SOIVIEYA1980 Volume 55 Issue 9 Pages 429-434
Published: September 15, 1980
Released on J-STAGE: May 24, 2011
JOURNAL FREE ACCESSExperimental studies on mycobacterial infection using inbred, specific pathogen free, germ-free and athymic “nude” mice performed by us during the past three decades at Department of Microbio logy and Gnotobiology Research Laboratory of our Institute of Public Health, were outlined.
Brief discussions were made on the susceptibility of various inbred mouse strains to tuberculous infection, the restoration of the anti-microbial resistance and delayed-type hypersensitivity of germ-free mice by the association of intestinal flora, recovery of anti-tuberculous immunity and granuloma forma tion in nude mice infected with mycobacteria by the transplantation with T-cell-containing lymphoid cells, and effects of H-2 gene histocompatibility between macrophages and T-lymphocytes on cellular immunity by transfer experiment in nude mice.
It was emphasized that the immunogenetical studies of tuberculosis using congenic mouse strains for H-2 gen complex; inbred mouse strains carrying defined haplotypes of H-2 gene complex from divers origins, should further be promoted.View full abstractDownload PDF (795K)
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