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Volume 65, Issue 8
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Takaichi TSUYUGUCHI1990 Volume 65 Issue 8 Pages 495-505
Published: August 15, 1990
Released on J-STAGE: May 24, 2011
JOURNAL FREE ACCESSImmunoaf f inity-purif ied antigens (MAb-Ags) of Mycobacterium tuberculosis, which had been obtained in a previous study by use of anti-M. tuberculosis monoclonal antibodies and designated MTA 2 a (24 kD), MTA 6 a (19 kD) and MTA 8 a (19 kD), were examined for their antigenicity by determining the responsiveness to these antigens of pulumonary tuberculosis (TB) patients and non-tuberculous controls. Serum anti-MAb-Ags ELISA antibody levels in TB patients were significantly higher than those in non-tuberculosis controls, including patients with atypical mycobacterial infection and Mantoux-testpositive healthy individuals. MAb-Ags stimulated proliferation of peripheral blood lymphocytes (PBL) from TB patients as determined by 3H-thymidine uptake of PBL. MAb-Ags also stimulated PBL from TB patients to increase T 4/T 8 ratio and Ia-positive T cells. After stimulation with MAb-Ags, increse of IL-2 receptor-positive T cells in PBL from TB patients was not significantly higher than that in PBL from healthy persons. Responses of PBL from non-tuberculosis controls to MAb-Ags varied considerably, and the response was either none or weak, in some cases, or as high as that of PBL from TB patients, in the other cases. In general, MAb-Ags stimulated PBL from TB patients and some of Mantoux test-positive persons to almost the same extent as PPDs did.View full abstractDownload PDF (1201K) -
A Gene Encoding 60kD Antigen (AT201) and Immunological Activity of Recombinant Peptides (15 and 60kD)Tomoko TANAKA-HAYASHI, Takaichi TSUYUGUCHI, Kazue AOYAMA, Haruki OKAMU ...1990 Volume 65 Issue 8 Pages 507-517
Published: August 15, 1990
Released on J-STAGE: May 24, 2011
JOURNAL FREE ACCESSTo obtain recombinant peptides related to PPDs, we constructed a genomic library from the DNA of Mycobacterium tuberculosis Aoyama B, a standard strain in Japan to manufacture PPDs, using plasmid vectors pUC18series. Seven clones reacting the anti-PPDs-rabbit-serum on immunoblotting were obtained, and restriction map was analysed.
A nucleotide sequence and a putative open reading frame (ORF) of pAT01, encoding 15kD peptide, as well as the mode of expression was reported previously. In this study, nucleotide sequence of 60kD peptide gene was determined, and the comparative database analysis (GENBANK) revealed a striking level of homology to so called mycobacterial heat shock protein. The expression mode of pAT201encoding 60kD, as well as pAT01enco 15kD peptide, indicated that these peptides were not hybrid proteins with the lacZ gene product, but they were consisted of peptides only mycobacterial source. Therefore, 15kD and60kD directly were subjected to immunological studies.
The peptides were extracted from E. coli, carrying pAT01or pAT201, purified through series of DEAE chromatography and followed by Detoxi-Gel to remove LPS. 15kD peptide behaved almost similar to PPDs both in the DTH skin reaction and the lymphocyte proliferation response on guinea pigs or rats in respect to sensitivity. However, 60kD was unique in that, and it behaved like a general mitogen.
We discussed role of 60kD peptide, comparing with the common antigen, generally found in most species of bacteria as the heat shock protein.View full abstractDownload PDF (1387K) -
Michio TSUKAMURA, Sadaaki YAMORI1990 Volume 65 Issue 8 Pages 519-525
Published: August 15, 1990
Released on J-STAGE: May 24, 2011
JOURNAL FREE ACCESSBacteriostatic and bactericidal activities of rifampicin, ethambutol, enviomycin and streptomycin on Mycobacterium avium-Mycobacterium intracellulare complex (MAI complex) strains were determined. The susceptibility testings were made in Ogawa egg medium, and minimal inhibitory concentrations (MICs) were determined as the lowest concentration of drugs, in which the growth of 20 to 100 colony-forming units was completely inhibited. The MICs correspond to the MICs that can inhibit the growth of 95 to 99% of bacterial population. Bactericidal activity was determined in a modified Dubos liquid medium (1.3g of Dubos TB Broth Base were dissolved in 180ml of distilled water and this solution was supplemented by 20ml of bovine serum). A one ml-sample of bacterial suspensions (10mg wet weight per ml) was added to 9ml of the Dubos liquid medium, and the medium was incubated at 37°C for 0, 1, 3 and 7 days under shaking condition (56 strokes per minute 8cm amplitude). The bactericidal activity was measured as the number of colony-forming units contained in a 0.02ml-sample of the medium.
The bactericidal activities of rifampicin and ethambutol were weak or absent even in strains 13008 and 13016, which were very susceptible to all four drugs. However, the bactericidal activities of streptomycin and enviomycin could be observed in these strains. In contrast, the bactericidal activities of all four drugs were absent or, if any, very weak in strains 13022, 13034 and 11004, which were naturally resistant to these drugs in the susceptibility testings, although the strains were isolated from patients who were untreated previously by any antituberculosis drugs or from fowl (strain 11004). On the other hand, the bactericidal activities of rifampicin, streptomycin and enviomycin were evident in Mycobacterium tuberculosis strain H37Rv. Only ethambutol was not bactericidal on this M. tuberculosis strain.View full abstractDownload PDF (670K) -
Masanobu FUJIOKA1990 Volume 65 Issue 8 Pages 527-537
Published: August 15, 1990
Released on J-STAGE: May 24, 2011
JOURNAL FREE ACCESSIn this study, 151 school children and students with tuberculosis registered in Aichi Prefecture during 1983 to 1987 were investigated.
The results were as follows:
1) The extent of lesions were not so advanced, as shown by the fact that 27.2% were cavitary and 31.8% were bacteriologically confirmed.
2) They were detected mainly by symptomatic visit to physicians (39.1%), periodic mass radiography (31.8%) and contacts survey (20.6%). The proportion of the former symptomatic visit was comparatively lower than in other age groups.
3) Patient's delay in cases detected by symptomatic visit was not so evident, however, there is a certain grade of doctor's delay; for instance, 22.0% of them took 2 months and more to be diagnosed.
4) 77.5% of them were vaccinated with BCG once or more, in most cases more than 5 years before their detection.
5) Sources of infection were specified in 52.3% of the cases. Most of them were found in their household and were bacteriologically confirmed at the time of detection. In some students their classmates were suspected as their possible source of infections.View full abstractDownload PDF (1147K) -
Naoto TOKUNAGA, Yoichiro ICHIKAWA, Mikio KUBOSHIRO, Toshihiro HIGASHI, ...1990 Volume 65 Issue 8 Pages 539-543
Published: August 15, 1990
Released on J-STAGE: May 24, 2011
JOURNAL FREE ACCESSWe reported a case of 64 year-old female patient of pulmonary tucercurosis associated with ARDS during corticosteroid treatment of Rheumatoid Arthritis. On admission her chief complaints were fever, fatigue and dyspnea. A chest roentgenogram showed diffuse alveolar infiltration consistent with pulmonary edema. Arterial blood gas studies showed severe hypoxemia. We clinicaly diagnosed so-called ARDS. Smears of sputum for acid fast bacilli were negative, but transbronchial lung brushing by bronchofiberscope revealed many acid fast bacilli. Intensive therapy with anti-tuberculosis drugs (INH, RFP, SM), high dose corticosteroid (methylpredonisolon) therapy and mechanical ventilation was started. During the following 2 weeks, the PaO2rose gradually and the alveolar infiltration on the chest roentgenogram disappeared. The experience of this case to emphasized the importance of suspecting this condition because pulmonary tuberculosis is a potentially curable cause of ARDS and it should also be emphasized that the good treatment effect could be expected with combined use of high dose corticosteor id and mechanical ventilation.View full abstractDownload PDF (1172K) -
Ichiro TOIDA, Setsuko YAMAMOTO1990 Volume 65 Issue 8 Pages 545-558
Published: August 15, 1990
Released on J-STAGE: May 24, 2011
JOURNAL FREE ACCESSDetermination of anti-mycobacteria antibody titers by ELISA for the early diagnosis of tuberculosis was critically reviewed from methodological point of view.
Especially, the method of data-handling to calculate the antibody titers from optical density values obtained by ELISA and also the method to evaluate the clinical usefulness of this test were reassessed.
Determination of anti-mycobacteria antibody titer by ELISA is highly sensitive and satisfactorily specific, and far more useful than, or at least as useful as, various on-going methods for the early diagnosis of pulmonary as well as extra-pulmonary tuberculosis.
This test should be included in a routine examination system for the diagnosis of tuberculosis in the clinical laboratories.View full abstractDownload PDF (1704K) -
[in Japanese]1990 Volume 65 Issue 8 Pages 561-562
Published: August 15, 1990
Released on J-STAGE: May 24, 2011
JOURNAL FREE ACCESSDownload PDF (256K)
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