Mushroom science and biotechnology
Online ISSN : 2433-0965
Print ISSN : 1340-7767
Volume 2 , Issue 4
Showing 1-3 articles out of 3 articles from the selected issue
  • Akiyoshi SAWABE, Masanori MORITA, Kazunari INABA, Seiji OUCHI, Tadashi ...
    Type: Article
    1995 Volume 2 Issue 4 Pages 157-166
    Published: December 30, 1995
    Released: March 14, 2018
    JOURNAL OPEN ACCESS
    odern mass spectrometry has original advantages in that it enables observation of molecular signals even of labile compounds, by means of "soft ionization" techniques such as fast atom bombardment (FAB), and allows characterization of individual molecules present in a mixture with the use of tandem mass spectrometry (MS/MS method) or B/E constant linked scan method. These methods are successfully applied to the determination of natural organic compounds, in particular. In this manuscript, recent works are reviewed focussing on the structural elucidation of lipids including new glycosyl phosphosphingolipids isolated from mushrooms by FAB mass spectrometry.
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  • Naoko AKIYAMA, Makoto YONEYAMA, Tomoko WATANABE, Akira SUZUKI
    Type: Article
    1995 Volume 2 Issue 4 Pages 167-175
    Published: December 30, 1995
    Released: March 14, 2018
    JOURNAL OPEN ACCESS
    Pileus color is not only an important item for quality evaluation of fruit bodies of Lentinula edodes in marketing, but also an obligation item for the registration of the new commercial strain to MAFF, Japan, under the seeds and seedlings law of Japan. In spite of its importance as an evaluation item, this color evaluation is carried out by comparing the test sample with the color standard sample by naked eye of skilled workers. The present experiments have been done to establish a more objective method for the evaluation of pileus color of L. edodes by using a color difference meter. The values of lightness, chroma, and hue-angle of pileus color at center, middle, and outer parts of pileus surface of L. edodes were determined. From the value obtained for Akiyama A-589go of the full-opened developmental stage (100% of development), which was grown under a normal air humidity (ca. 65-90% RH), using a 6-mm diameter measurement field of the meter, the possibility of a normal distribution was not excluded at the 5% level by chi-square test for goodness of fit, while those with a 30 mm-diameter measurement field did not fit a normal distribution at the 0.3% level. Therefore, a 6 mm-diameter measurement field was appllied as the standard size for the measurement field in the following experiments. It was demonstrated that the changes in the colors of the pileus surface of the strains, A-567 and Akiyama A-500go during pileus development reflected the values of lightness and chroma. When the colors of pileus surface among 6 tested strains (A-567, A-580, Akiyama A-503go, Akiyama A-589go, Akiyama A-500go, and Akiyama A-550go) at the 80% development (the standard developmental stage of fruit bodies for qualifing the new commercial strain of L. edodes for the registration to MAFF, Japan) were compared, the differences of values of lightness, chroma, and hue-angle of pilei among the cultures tend to become greater at the outer part. The pileus surface of fruit bodies of A-580 grown under a high humidity condition (ca. 95-100% RH.) gave lower values of lightness and chroma than those from fruit bodies grown under a normal condition. The values of lightness, chroma and hue-angle of pileus colors for each strain in the present experiments measured with the color difference meter well corresponded to the determination of the pileus color by naked eye. These results suggests that the color difference meter could be applied for the objecive evaluation of color of fresh pileus in L. edodes, when the outer part of pileus at a certain developmental stage was measured by using a 6 mm-diameter measurement field.
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  • [in Japanese], [in Japanese]
    Type: Article
    1995 Volume 2 Issue 4 Pages 177-181
    Published: December 30, 1995
    Released: March 14, 2018
    JOURNAL OPEN ACCESS
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