The Keio Journal of Medicine
Online ISSN : 1880-1293
Print ISSN : 0022-9717
ISSN-L : 0022-9717
Volume 25, Issue 4
Displaying 1-5 of 5 articles from this issue
  • YASUO IKEDA
    1976 Volume 25 Issue 4 Pages 139-150
    Published: 1976
    Released on J-STAGE: March 27, 2009
    JOURNAL FREE ACCESS
    The conditions for assembly-disassembly of platelet microtubules were evaluated using a highly purified tubulin preparation obtained from human platelets. The extent of polymerization could be measured by recording the increase in optical absorbance at 500 nm. Electron microscopy verified the tubular nature of the polymerized tubulin. Maximal polymerization was directly proportional to the protein concentration and the temperature of the system. Rate and magnitude of polymerization at 37°C was significantly increased by addition of GTP or ATP. Cyclic nucleotides did not influence upon this process. Storage at 4°C rapidly decreased the ability of tubulin to polymerize. The method was developed to evaluate a dynamic equilibrium in vivo between microtubules and subunit protein (tubulin) of which they are formed. Chilled platelets showed significantly decreased amount of microtubules concomitantly associated with increased tubulin. It is suggested that this method could be widely utilized to evaluate such a shift of equilibrium in platelets, which may affect several important platelet functions.
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  • YOSHIO KOBAYASHI
    1976 Volume 25 Issue 4 Pages 151-162
    Published: 1976
    Released on J-STAGE: March 27, 2009
    JOURNAL FREE ACCESS
    The combined effect of amikacin and colistin on Pseudomonas aeruginosa was studied by the agar plate two-fold dilution method. The author further studied this effect by using the hole plate method.
    Inhibition by amikacin at 3.13 μg/ml of gentamicin resistant P. aeru-ginosa was much more remarkable when amikacin was applied alone than in cases where it was used in combination with colistin. The concomitant use of colistin at concentractions of one-eighth to one-half the MIC of colistin increased the concentration required to inhibit the growth of the strains to twice the MIC of amikacin.
    Bacteriostatic antagonism was thus detected between amikacin and colistin against gentamicin resistant P. aeruginosa. The author assumed that this phenomenon was mainly due to a lowered activity of amikacin rather than of colistin. The above mentioned phenomenon was then confirmed by the hole plate method. Such bacteriostatic antagonism was also detected between amikacin and colistin as well as between gentamicin and colistin when used against gentamicin sensitive P. aeruginosa.
    The author suggests, based on his in vitro observations, that the combined use of these antibiotics should not be taken as clinical practice.
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  • MASAAKI NAKAZONO, SHOZO IWATA, KEIZO SUZUKI
    1976 Volume 25 Issue 4 Pages 163-167
    Published: 1976
    Released on J-STAGE: March 27, 2009
    JOURNAL FREE ACCESS
    Minute leiomyomatous nodules are found rather commonly in benign hyperplasia of the prostate. But true leiomyomas of the prostate, which can meet the definitions proposed by Kaufman and Bernike, are rare. In Japan 5 cases are found in the literatures.
    An additional case, a male 52-years-old complaining retention of urine was reported. Preoperative diagnosis was benign hyperplasia of the prostate and was exposed by the usual suprapubic approach. The tumor was too large to resect en block, but the operation finished uneventfully. The surgical specimen weighed 420g that was the largest one reported in Japan. The postoperative course was an acceptable one. The final pathological diagnosis was leio-myoma of the prostate. The details are described herein.
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  • TOSHIHIKO HASEGAWA
    1976 Volume 25 Issue 4 Pages 169-192
    Published: 1976
    Released on J-STAGE: March 27, 2009
    JOURNAL FREE ACCESS
    Clinical, cytologic, histologic and electron-microscopic studies on cryo-surgery for the treatment of chronic cervicitis were carried out in 119 patients who were suffering from heavy discharge and/or contact bleeding due to wide erosion. Healing was documented in 92% without serious side effects. Repeat cryosurgery was required to control bleeding in 2 cases and a case of recurrent adnexitis was observed. Atypias were present in 19% of cytological specimen obtained during follow-up. Cytomorphological anal-ysis revealed atypical cells showed identical features observed in tissue re-pair cells. Healing process took place initially on both ecto- and endocervix with covering the denuded area with mono-layered cells. It was suggested that main process of epidermization was reserve cell hyperplasia-squamous metaplasia-squamous epithelium. Electron-microscopic investigation re-vealed that severe damages were present immediately after freezing in cells of squamous epithelium which looked almost unchanged by light microscopic observation. Mono-layered cells showed some features of glandular cells and were not clearly identified as so called reserve cells. Superficial part of immature squamous metaplasia consisted of cells with rough endoplasmic reticulum with widely dilated lumen, free ribosome and microvilli with sur-face coating substance. Basal cells showed features of squamous epithelium.
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  • KENZABURO TSUCHIYA, MINORU SUGITA, C.B. PARK
    1976 Volume 25 Issue 4 Pages 193-211
    Published: 1976
    Released on J-STAGE: March 27, 2009
    JOURNAL FREE ACCESS
    The purpose of this study was to examine whether the health of Tokyo inhabitants was affected by lead in the ambient air, on the bases of place of residence, age and sex. The study came about as a direct result of public concern over a press report that people living near busy traffic intersections were being poisoned by lead in the air. In 1970 and 1971, the laboratories of the Tokyo Metropolitan Institute of Health and the Occupational Health Service Center performed laboratory tests on inhabitants who were concerned about air pollution and presented themselves to their local health departments. The results were then passed on to the Keio University School of Medicine for evaluation.
    The average lead levels in blood and urine were slightly higher in those person living in houses which faced a main street (13.3 μg/100ml blood) than in those who living on side streets (12.8 μg/100ml). However, there was no association found to exist according to the distance of the house from the main street. Blood lead levels were higher in males (15.0 μg/100ml) than in females (11.2 μg/100 ml). Lead levels in urine were also higher in males than in females: 13.1 μg/100 ml as opposed to 11.7 μg/100ml. There was no particular association between age and lead levels in blood or urine.
    The average lead levels in blood and urine differed by district but showed no systematic pattern or relationship to the population density. This seems to be due to technical differences between the two laboratories responsible for the testing as well as to the difficulty in determining such small amounts of lead in blood and urine.
    In conclusion, according to the available techniques, there was no evidence of an increased level of lead in blood or urine as a result of lead in the ambient air of Tokyo. The results obtained in this study agree with a previous report by the present authors' and with a report by Tepper and Levin2 from the U.S.A.
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