The Keio Journal of Medicine
Online ISSN : 1880-1293
Print ISSN : 0022-9717
ISSN-L : 0022-9717
Volume 29, Issue 4
Displaying 1-5 of 5 articles from this issue
  • TSUNEHISA MAKINO, MITSUTOSHI IWASHITA, AKIHIKO NAKAYAMA, TSUNEO YOKOKU ...
    1980 Volume 29 Issue 4 Pages 141-151
    Published: 1980
    Released on J-STAGE: March 27, 2009
    JOURNAL FREE ACCESS
    The hypothalamo-pituitary function during pregnancy was investigated by analysing blood levels of Luteinizing Hormone Releasing Factor (LH-RF) and LH-beta subunit. A specific and sensitive radioimmunoassay which can detect as small as 300 femtogram of LH-RF per tube was developed. This re-vealed that plasma LH-RF concentrations were 1.0 ± 0.14pg/ml (mean ± S.E.) at follicular phase (n=17) and 1.1 ± 0.19pg/ml at luteal phase (n=8), whereas LH-RH was detectable in only 3 out of 20 samples from pregnant women and mean values were significantly decreased to 0.4 ± 0.02pg/ml. Simi-larly serum hLH beta levels were 438.56 ± 49.30 ng/ml at follicular phase (n=33), 632.71 ± 75.09ng/ml at mid-cycle (n=21) and 258.55 ± 46.84ng/ml at luteal phase (n=20), respectively. The levels of this hormone were also markedly decreased to 11.81 ± 0.95ng/ml during pregnancy (n=129).
    The data indicate that the hypothalamo-pituitary function, in terms of the hormones measured in this study, is lowered during pregnancy, presumably by the influences of placental hormones.
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  • AKIHIKO NAKAYAMA, TSUNEHISA MAKINO, TAKASHI NAGAI, KAZUMI NAKAZAWA, HI ...
    1980 Volume 29 Issue 4 Pages 153-161
    Published: 1980
    Released on J-STAGE: March 27, 2009
    JOURNAL FREE ACCESS
    To clarify the hypothalamo-posterior pituitary function during pregnancy, puerperium and menstrual cycle, investigations were performed to determine the levels of serum neurophysins by specific radioimmunoassays. Sera from 75 women during normal pregnancy, 17 during postpartum and 49 during the normal menstrual cycle were assayed for Estrogen Stimulated Neuro-physin (ESN) and Nicotine Stimulated Neurophysin (NSN). ESN, associated with oxytocin increased during pregnancy and the values decreased to non-pregnant levels after parturition. During menstrual cycle ESN showed three peaks. In contrast, NSN, associated with vasopressin was little changed throughout the menstrual cycle, pregnancy and the puerperium. In this study two neurophysins changed independently, suggesting the hypothesis of “one hormone-one neurophysin.” The data also would indicate the availability of measurement of these two substances on the study of the function of human neurosecretory system.
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  • I. CLASSIFICATION OF ACUTE MYELOGENOUS LEUKEMIA
    JUNSEI WADA
    1980 Volume 29 Issue 4 Pages 163-174
    Published: 1980
    Released on J-STAGE: March 27, 2009
    JOURNAL FREE ACCESS
    Twenty three cases of acute myelogenous leukemia (AML) were in-vestigated by ultrastructural peroxidase cytochemistry. The 23 cases were classified into two groups, Group A and B. In Group A (7 cases), the pre-dominant leukemic cells were without granules, while in Group B (16 cases), the predominant cells were with granules. Leukemic cells without granules showed peroxidase reaction product in the nuclear envelope and rough endo-plasmic reticulum (RER). These cells were identified as leukemic myeloblasts. Leukemic cells with granules were recognized as leukemic promyelocytes by the presence of peroxidase reaction product in the nuclear envelope, RER, Golgi apparatus and all cytoplasmic granules. There was no significant difference in the granule size of leukemic promyelocytes between Group A and B (150-400 nm: the smallest diameter in a thin section). The leukemic promyelocyte of Group A, however, exhibited a higher nucleo-cytoplasmic ratio and a fewer number of granules than those of Group B, indicating that the leukemic pro-myelocyte of Group A was at an earlier stage of granule formation.
    These findings suggest that the predominant cells in Group A are of an immature type and those in Group B of a mature type. Clinically, the patients in Group A had a higher incidence of complete remission and a longer median survival time than those belonging to Group B.
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  • II. BLAST CRISIS OF CHRONIC MYELOGENOUS LEUKEMIA
    JUNSEI WADA
    1980 Volume 29 Issue 4 Pages 175-183
    Published: 1980
    Released on J-STAGE: March 27, 2009
    JOURNAL FREE ACCESS
    Eight cases of Philadelphia chromosome positive chronic myelogenous leukemia (CML) in blast crisis have been studied by ultrastructural per-oxidase cytochemistry. In 7 cases, peroxidase reaction product was seen in the nuclear envelope and rough endoplasmic reticulum (RER) of the blasts. These cells had no cytoplasmic granules. In 6 cases, a few cells also showed peroxidase reaction product in the nuclear envelope, RER, and all cytoplasmic granules.
    In contrast, peroxidase reaction product was not shown in the nuclear envelope and RER in all blasts in one case. The blasts did not have granules. Although these cells could not be identified, the high level of terminal deoxy-nucleotidyl transferase (TdT) activity suggested this case had undergone ‘lymphoid’ crisis.
    Ultrastructural peroxidase cytochemistry is, therefore, useful for the identification of blast cells in CML-blast crisis.
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  • TOSHIHIKO ARAI, SADAO KOMATSU, YOKO KOMATSU, AKIO KOBAYASHI
    1980 Volume 29 Issue 4 Pages 185-196
    Published: 1980
    Released on J-STAGE: March 27, 2009
    JOURNAL FREE ACCESS
    Changes of resistant Escherichia coli strains and their conjugative R plasmids followed by the changes of chemotherapy occurred in Japan were described. The chemotherapy in Japan has changed from parallel usages of various drugs to the extensive and high dose usages of β-lactam antibiotics after 1975. As expected, β-lactam antibiotic resistant strains increased great-ly. But conjugative R plasmids have had rather qualitative changes, that is, conjugative R plasmids seemed to receive rearrangements of their genes and become fewer drug resistant forms or non (less) -conjugative forms from multiple drug resistant conjugative forms.
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