The Keio Journal of Medicine
Online ISSN : 1880-1293
Print ISSN : 0022-9717
ISSN-L : 0022-9717
Volume 32, Issue 3-4
Displaying 1-5 of 5 articles from this issue
  • KEIJI SHIODA, FUJIO ISEKI, JIRO KUBOI, TAISUKE TOMATSU, TSUYOSHI TAKED ...
    1983 Volume 32 Issue 3-4 Pages 59-71
    Published: 1983
    Released on J-STAGE: March 27, 2009
    JOURNAL FREE ACCESS
    In order to learn how pathological changes and symptoms of gonarthritis progress over a long period of time, we selected those cases having a history for over 10 years, in which the patient had not received surgical operation, as the subjects of our investigation.
    The subject cases including 96 joints were investigated to find out the correlations between the progress of pathological change and such factors as sex, age, obesity, occupation, way of living, type of toilet, hospital visit, or FTA (femoro-tibial angle) etc. The results were as follows:
    (1) Changes at the medial condyle get most easily aggravated, which are followed by those occurred at the patellofemoral joint and the aggravation is the least in changes at the lateral condyle.
    (2) Obesity as opposed to normal makes the grade of gonarthritis worse.
    (3) There are no correlations between aggravation of gonarthritis and age, sex, way of living or occupation.
    (4) In advanced cases, patients use western style toilets.
    (5) In advanced cases, patients visit hospital more frequently.
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  • AKIRA ONO, HIDETSUGU SAITO, TORU HIGASHINAKAGAWA, TATSUYA NAKAMURA, SH ...
    1983 Volume 32 Issue 3-4 Pages 73-80
    Published: 1983
    Released on J-STAGE: March 27, 2009
    JOURNAL FREE ACCESS
    We have investigated three human fetal thymuses by various induction procedures in a search for retrovirus. Thymus cells were cultured for 7 days together with mitomycin C-treated B cells and examined for reverse transcriptase activity. The following results were obtained. 1) RNA-directed DNA polymerase (RDDP) activity was detected in the culture medium on day 3 and persisted until day 7. This enzyme, after partial purification on phosphocellulose column, showed template-primer specificity of a typical reverse transcriptase. 2) In unstimulated thymus cells, no reverse transcriptase activity was detected. 3) Stimulation with lymphocyte mitogens such as phytohaemagglutinin (PHA) and concanavalin A (Con A) exerted no effect on inducing the reverse transcriptase activity. 4) No reverse transcriptase activity was obtained in the culture medium of B cells pretreated with mitomycin C. Taken together these results indicate that in human fetal thymus cells reverse transcriptase activity is induced by human B cell stimulation, pointing to an intriguing possibility of protovirus activation.
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  • CYTOTOXIC ACTIVITY TO HUMAN OVARIAN CANCER CELL LINES AND REACTIVITY TO PHYTOHEMAGGLUTININ SHOWN BY LYMPHOCYTES
    YOSHIO FUKUDA
    1983 Volume 32 Issue 3-4 Pages 81-98
    Published: 1983
    Released on J-STAGE: March 27, 2009
    JOURNAL FREE ACCESS
    Peripheral blood lymphocytes collected from 23 healthy female volunteers, and 76 patients with gynecologic tumors including 33 ovarian cancers and 23 uterine cancers, 99 in total, was used to perform the allogeneic cytotoxicity test (to be referred to as “the test”) and to evaluate for the blastogenic response to phytohemagglutinin (to be referred to as “the response”). The test was performed according to the method by Takasugi and Klein. Target cells used were two cell lines derived from human ovarian cancer, namely, HOC-21 from serous cystadenocarcinoma and YS-K from yolk sac tumor. The response was assayed on lymphocytes separated from the peripheral blood and on a very small amount of heparinized whole blood.
    In the test, the higher the concentration of lymphocyte, the more cytotoxically it functioned. The cytotoxicity of lymphocytes from the patients with ovarian tumors, particularly ovarian cancers, was found to be higher than that from the patients with uterine tumors. Also, high cytotoxic activities were seen in the patients with dermoid cysts of ovary and choriocarcinomas. However, it was noted that the cytotoxicity was not always high in the patients whose histologic type was the same as that of the target cell. Cytotoxicity showed a slight decrease along with a progress of disease, but it was rather higher in recurrent cases.
    By whole blood assay, the response was lower in the patients with malignant tumors than in the patients with benign tumors, and it was significantly low in advanced and recurrent cases. However, it was as normal as in the healthy subjects by lymphocyte assay.
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  • SHIGERU KURAMOCHI
    1983 Volume 32 Issue 3-4 Pages 99-111
    Published: 1983
    Released on J-STAGE: March 27, 2009
    JOURNAL FREE ACCESS
    Five autopsy cases showing more than 20 hepatocellular mitotic figures per 1cm2 of liver parenchyma had also widespread skeletal muscle necrosis and renal failure. Myoglobin casts in renal tubules were remarkable. In three of these cases liver cell damage was obvious, but in two others it was negligible. Mechanisms of muscle necrosis were probably different from case to case. For a control study, 32 autopsy cases were selected on the basis of underlying diseases such as acute or chronic liver disease, acute or chronic renal failure, polymyositis with or without muscle necrosis at autopsy, etc., and the frequency of hepatocellular mitosis was measured. All the control cases had less than 5 mitoses per 1cm2 of liver parenchyma. In cases showing muscle necrosis in the control study, it was considerably less severe than that in the cases showing numerous mitoses. The present study confirms that the appearance of a considerable number of hepatocellular mitoses is an unusual finding even in conditions in which it might be expected, and is independent of liver cell damage. In some cases it might be related to widespread muscle necrosis. It is suggested that pathologists should examine muscles histologically whenever they observe numerous liver cell mitoses in autopsy cases. The nature and significance of an excess of hepatocellular mitoses at autopsy, whether due to the actual induction of mitotic activity, or representing the accumulation of mitotic figures as the result of the inhibition of cell division after death are discussed in relation to muscular necrosis.
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  • BAO-LIANG LIN, TSUNEHISA MAKINO, HIROAKI SUEKANE, MASAYOSHI ARISAWA, M ...
    1983 Volume 32 Issue 3-4 Pages 113-121
    Published: 1983
    Released on J-STAGE: March 27, 2009
    JOURNAL FREE ACCESS
    To investigate both the release and synthesis of gonadotropins in the anterior pituitary gland, twice administrations of synthetic LH-RH at 60 minute interval on 19 normal cycling and 5 postmenopausal women and 5 male adults were performed. Serum LH and FSH of blood samples collected at 0', 30', 60', 90' and 120' were measured by radioimmunoassays and the pituitary functions for gonadotropin release (Δ1) and synthesis (Δ2) were analysed.
    In preovulatory phase, postmenopausal women and adult males, of of both LH and FSH were dominant than Δ2 while Δ1 were smaller than Δ2 in the groups of follicular phase and luteal phase. These results indicated that LH-RH two step test was a more useful method as compared to single LH-RH test to investigate both release and synthesis of pituitary gonadotropic hormones.
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