The Keio Journal of Medicine
Online ISSN : 1880-1293
Print ISSN : 0022-9717
ISSN-L : 0022-9717
Volume 33, Issue 1
Displaying 1-4 of 4 articles from this issue
  • YUTAKA TAKASHIMA, TAKEHARU FURUKAWA
    1984 Volume 33 Issue 1 Pages 1-16
    Published: 1984
    Released on J-STAGE: March 27, 2009
    JOURNAL FREE ACCESS
    As slight abnormal values of serum GOT and GPT levels are observed very often in health examinations, we studied the effects of overweight or habitual drinking on the two transaminase levels using data from health examinations of male clerical workers aged 40-54.
    The serum GPT level had a linear positive correlation with the obesity index (p<0.01), whereas association of the obesity index and serum GOT level was not clear (p>0.1). The condition in which the serum GPT level exceeds the serum GOT level (GOT/GPT<1) and both of them are less than 100 units, was found to be largely due to overweight or obesity. The condition in which the serum GOT level exceeds the serum GPT level and the former is moderately elevated in the range of 30 to 100 units, was strongly associated with heavy drinking.
    We conclude that effective guidelines, based on slight changes in serum transaminase levels, can be provided to obese individuals or drinkers in order to prevent advanced liver cell injuries.
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  • HITOSHI HANAUE, TSUTOMU KUROSAWA, HIDEKI YASUDA, SATOSHI YOSHIZAKI, SH ...
    1984 Volume 33 Issue 1 Pages 17-27
    Published: 1984
    Released on J-STAGE: March 27, 2009
    JOURNAL FREE ACCESS
    Nonspecific immunotherapy with OK-432, penicillin and heat treated lyophilized powder of Su-strain of streptococcus pyogenes A3, was evaluated in patients with recurrent or unresectable malignant tumors at various digestive organs to assess the relative benefit of the preparation administered by different routes. Comparative studies were made of the survival period, the variation in immunological parameters, and the incidence of adverse reactions in two groups of patients with uniform background factors: 30 receiving the preparation intradermally and 21 receiving intramuscular doses of the preparation. The survival rate was significantly higher (P=0.001) for patients receiving intradermal than those receiving intramuscular doses of the preparation. In the former group, serious adverse reaction was not found but more marked improvement was achieved in various immunological parameters examined. The results of the present study showed that the preparation is of greater value when it is administered intradermally than intramuscularly to treat malignant tumor at various digestive organs.
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  • KATSUMI YOSHIDA, HARUHIKO SAKURAI
    1984 Volume 33 Issue 1 Pages 29-38
    Published: 1984
    Released on J-STAGE: March 27, 2009
    JOURNAL FREE ACCESS
    To clarify the quantitative relationship between lead exposure and anemia, a cohort of 179 newly-employed male workers was observed for 5 years after their placement in lead-exposed jobs. The examined items included specific gravity of blood (SG-B), urinary D-aminolevulinic acid (ALA-U), urinary coproporphyrin (CP-U) and blood lead concentration. The blood lead concentration ranged from less than 10μg/100ml up to 80μg/100ml.
    There were three findings which justified the conclusion that lead exposue may not induce manifest anemia at the level of exposure with blood lead concentration not exceeding 80μg/100 ml. First, SG-B had no association with blood lead concentration, while ALA-U exhibited a dose-related increase. Secondly, SG-B did not have a negative correlation with blood lead level (r=0.116, p<0.01) and stepwise regression analysis also indicated that blood lead had a slightly positive coefficient as an independent variable. Thirdly, when a casecontrol analysis was applied, there were no differences in ALA-U, CP-U and blood lead levels between low SG-B workers and their matched controls.
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  • MITSUNOBU SASAKI
    1984 Volume 33 Issue 1 Pages 39-56
    Published: 1984
    Released on J-STAGE: March 27, 2009
    JOURNAL FREE ACCESS
    In order to identify tumor associated antigen (TAA) on the cell surface of transitional cell carcinoma (TCC) of the bladder, monoclonal antibodies (Mabs) to the established cell line KU-1, derived from transitional cell carcinoma of the bladder, was produced. Cell fusion technique, utilizing spleen cells of immunized Balb/c mouse and SP-2 cells of mouse myeloma cell line, was applied in the present study.
    Identification and quantification of cell surface antigen of KU-1 cells and another established cell line derived from bladder carcinoma KU-7 cells were attempted by determining reactivity of the cell surface antigens with thus produced Mabs utilizing enzyme linked immunosorbent assay (ELISA).
    Immunohistochemical study demonstrated the cell surface antigen on these two cell lines and surgically removed fresh specimens of TCC of the bladder, using indirect immunofluorescence technique.
    Two distinct Mabs (TBSN-1 and TBSN-2) were obtained. TBSN-1 diluted ten fold was observed to be reactive with KU-1 cells but not with KU-7 cells. TBSN-2 diluted twenty fold showed reactivity with KU-7 cells but not with KU-1 cells.
    Complement mediated cytotoxicity of these two Mabs against these two cell lines showed higher cytotoxicity of TBSN-1 against KU-7 cells (97.3±15.1% at 1:1 relative concentration of complement) as compared with its cytotoxicity against KU-1 cells (54.2±5.1%). Correlation between complement dose and cytotoxicity of TBSN-1 was statistically significant. TBSN-2 showed markedly higher cytotoxicity against KU-1 cells (57.8±10.5%) than against KU-7 cells (22.2±3.7%), however there was no correlation between complement dose and cytotoxicity of TBSN-2.
    Cross reactivity of the Mabs showed higher specificity of TBSN-2 to TCC of the bladder.
    The present study disclosed the presence of specific Mab (TBSN-2) to TCC of the bladder. In spite of different histopathological grade of the original tumors, both KU-1 and KU-7 had tumor associated antigen capable of binding with TBSN-2.
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