When super-immunized mice with living vaccine were challenged with 10
4 cells of virulent strain of
S.enteritidis via intracutaneous route, the bacteria increased rapidly for 24 hours, then decreased slowly in the skin, finally they disappeared. The bacteria were isolated also from local lymph nodes, but scarcely from the liver.
In the case of a small challenge dose (10
2 cells), the bacteria survived in the skin but were not found in the local lymph nodes, blood and liver.
The other mice were immunized with killed vaccine intra-cutaneously. when the challenge site of the skin differed from the immunized area, the challenged bacteria increased in she skin during the first 24 hours. The degree of initial increase of bacteria was similar to that in the case of the normal mice or super-immunized mice.
When the bacteria were injected at the same point of the immunized skin, however, the bacteria decreased for the first 6 hours in the skin and then increased rapidly for a few days. In the later stadium, the difference of injected site had no different effect on the concentration of bacteria in the skin.
It was considered that this decrease in bacterial number related not only to the antibody titer in the serum but also to the localized proliferation of hysteocytes after the vaccination.
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